PSMA7 Antibody (1A10-3G12) - Azide and BSA Free

Novus Biologicals | Catalog # H00005688-M01

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1A10-3G12

Format

Azide and BSA Free
View all PSMA7 Primary Antibodies »

Product Specifications

Immunogen

PSMA7 (AAH04427, 1 a.a. ~ 248 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGVRGRDIVVLGVEKKSVAKLQDERTVRKICALDDNVCMAFAGLTADARIVINRARVECQSHRLTVEDPVTVEYITRYIASLKQRYTQSNGRRPFGISALIVGFDFDGTPRLYQTDPSGTYHAWKANAIGRGAKSVREFLEKNYTDEAIETDDLTIKLVIKALLEVVQSGGKNIELAVMRRDQSLKILNPEEIEKYVAEIEKEKEENEKKKQKKAS

Specificity

PSMA7 - proteasome (prosome, macropain) subunit, alpha type, 7

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PSMA7 Antibody (1A10-3G12) - Azide and BSA Free

Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01]

Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01]

Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of PSMA7 expression in transfected 293T cell line by PSMA7 monoclonal antibody (M01), clone 1A10-3G12.Lane 1: PSMA7 transfected lysate (Predicted MW: 27.9 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PSMA7 Antibody (1A10-3G12) [H00005688-M01]

Immunocytochemistry/ Immunofluorescence: PSMA7 Antibody (1A10-3G12) [H00005688-M01]

Immunocytochemistry/Immunofluorescence: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of monoclonal antibody to PSMA7 on HeLa cell. Antibody concentration 1 ~ 10 ug/ml.
Immunohistochemistry-Paraffin: PSMA7 Antibody (1A10-3G12) [H00005688-M01]

Immunohistochemistry-Paraffin: PSMA7 Antibody (1A10-3G12) [H00005688-M01]

Immunohistochemistry-Paraffin: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human colon tissue. Antibody concentration 5 ug/ml.
Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01]

Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01]

Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - PSMA7 monoclonal antibody (M01), clone 1A10-3G12 Analysis of PSMA7 expression in HeLa.
Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01]

Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01]

Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - PSMA7 monoclonal antibody (M01), clone 1A10-3G12. Analysis of PSMA7 expression in human omentum, serous carcinoma.
Immunohistochemistry-Paraffin: PSMA7 Antibody (1A10-3G12) [H00005688-M01]

Immunohistochemistry-Paraffin: PSMA7 Antibody (1A10-3G12) [H00005688-M01]

Immunohistochemistry-Paraffin: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. Antibody concentration 5 ug/ml.
Immunoprecipitation: PSMA7 Antibody (1A10-3G12) [H00005688-M01]

Immunoprecipitation: PSMA7 Antibody (1A10-3G12) [H00005688-M01]

Immunoprecipitation: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of PSMA7 transfected lysate using anti-PSMA7 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PSMA7 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Detection limit for recombinant GST tagged PSMA7 is approximately 1ng/ml as a capture antibody.

Applications for PSMA7 Antibody (1A10-3G12) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recominant protein, tissue lysate and cell lysate for WB. It has also been used for IF, IHC-P and ELISA.

Reviewed Applications

Read 1 review rated 5 using H00005688-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PSMA7

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. This particular subunit has been shown to interact specifically with the hepatitis B virus X protein, a protein critical to viral replication. In addition, this subunit is involved in regulating hepatitis virus C internal ribosome entry site (IRES) activity, an activity essential for viral replication. This core alpha subunit is also involved in regulating the hypoxia-inducible factor-1alpha, a transcription factor important for cellular responses to oxygen tension. Multiple isoforms of this subunit arising from alternative splicing may exist but alternative transcripts for only two isoforms have been defined. A pseudogene has been identified on chromosome 9.

Long Name

Proteasome subunit alpha type-7

Alternate Names

HSPC

Entrez Gene IDs

5688 (Human)

Gene Symbol

PSMA7

OMIM

606607 (Human)

UniProt

AAH04427 (Human)

Additional PSMA7 Products

Product Documents for PSMA7 Antibody (1A10-3G12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PSMA7 Antibody (1A10-3G12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PSMA7 Antibody (1A10-3G12) - Azide and BSA Free

Customer Reviews for PSMA7 Antibody (1A10-3G12) - Azide and BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: HeLa cell lysates
    Species: Human
    Verified Customer | Posted 01/24/2012
    PSMA7 Antibody (1A10-3G12) - Azide and BSA Free H00005688-M01

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Protocols

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