PTP1B/PTPN1 Antibody (OTI1D10)

Novus Biologicals | Catalog # NBP2-01056

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Rat, Canine, Monkey, Primate

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1D10
View all PTP1B Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PTPN1(NP_002818) produced in HEK293 cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

49.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PTP1B/PTPN1 Antibody (OTI1D10)

Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]

Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]

Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and PTPN1-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-PTPN1 monoclonal antibody. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody as a loading control (1:500). Image using the Azide and BSA Free format of this antibody.
Immunocytochemistry/ Immunofluorescence: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]

Immunocytochemistry/ Immunofluorescence: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]

Immunocytochemistry/Immunofluorescence: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPN1.
Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056] - Staining of paraffin-embedded Human lymphoma tissue using anti-PTPN1 mouse monoclonal antibody.
Flow Cytometry: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]

Flow Cytometry: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]

Flow Cytometry: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PTPN1 antibody, and then analyzed by flow cytometry.
Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]

Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]

Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN1.
Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]

Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]

Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056] - Analysis of extracts (35ug) from 9 different cell lines by using anti-PTPN1 monoclonal antibody.
Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056] - Staining of paraffin-embedded Human colon tissue using anti-PTPN1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056] - Staining of paraffin-embedded Human lymph node tissue using anti-PTPN1 mouse monoclonal antibody.

Applications for PTP1B/PTPN1 Antibody (OTI1D10)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PTP1B

The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. [provided by RefSeq].

Long Name

Protein Tyrosine Phosphatase 1B

Alternate Names

PTPN1

Entrez Gene IDs

5770 (Human)

Gene Symbol

PTPN1

Additional PTP1B Products

Product Documents for PTP1B/PTPN1 Antibody (OTI1D10)

Certificate of Analysis

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Product Specific Notices for PTP1B/PTPN1 Antibody (OTI1D10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Phosphatases and Regulators

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Protocols

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