PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-73729

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Rat, Canine, Monkey, Primate

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1D10

Format

Azide and BSA Free
View all PTP1B Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PTPN1(NP_002818) produced in HEK293 cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

49.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free

Western Blot: PTP1B/PTPN1 Antibody (OTI1D10)Azide and BSA Free [NBP2-73729]

Western Blot: PTP1B/PTPN1 Antibody (OTI1D10)Azide and BSA Free [NBP2-73729]

Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN1.
Immunocytochemistry/ Immunofluorescence: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]

Immunocytochemistry/ Immunofluorescence: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]

Immunocytochemistry/Immunofluorescence: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPN1.
Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729] - Staining of paraffin-embedded Human lymphoma tissue using anti-PTPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.
Flow Cytometry: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]

Flow Cytometry: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]

Flow Cytometry: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729] - HEK293T cells transfected with either RC204902 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PTPN1 antibody and then analyzed.
Western Blot: PTP1B/PTPN1 Antibody (OTI1D10)Azide and BSA Free [NBP2-73729]

Western Blot: PTP1B/PTPN1 Antibody (OTI1D10)Azide and BSA Free [NBP2-73729]

Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729] - Analysis of extracts (35ug) from 9 different cell lines by using anti-PTPN1 monoclonal antibody.
Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729] - Staining of paraffin-embedded Human colon tissue within the normal limits using anti-PTPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.
Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729] - Staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PTPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.
Knockout Validated: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]

Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729]

Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and PTPN1-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-PTPN1 monoclonal antibody. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody as a loading control.(1:500)F

Applications for PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: PTP1B

The non-transmembrane protein tyrosine phosphatase, PTP1B, comprises 435 amino acids, of which the C-terminal 114 residues have been implicated in controlling both localization and function of this enzyme. Increased phosphorylation of PTP1B is seen to accompany the transition from G2 to M phase of the cell cycle. Immunofluorescence experiments indicate that PTP-1B is localized predominantly in the endoplasmic reticulum (ER). Subcellular fractionation is consistent with this localization and establishes that PTP-1B is tightly associated with microsomal membranes, with its phosphatase domain oriented towards the cytoplasm. The finding of a tyrosine phosphatase on the ER suggests new possibilities for cellular events controlled by tyrosine phosphorylation (2). It has also been shown that PTP1B is a novel substrate for Akt and that phosphorylation of PTP1B by Akt at Ser(50) may negatively modulate its phosphatase activity creating a positive feedback mechanism for insulin signaling (3).

Long Name

Protein Tyrosine Phosphatase 1B

Alternate Names

PTPN1

Gene Symbol

PTPN1

Additional PTP1B Products

Product Documents for PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Phosphatases and Regulators

Customer Reviews for PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free and earn rewards!

Have you used PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies