Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PTRH2 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide from near the center of human PTRH2. The immunogen is located within amino acids 80 - 130 of PTRH2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PTRH2 Antibody - BSA Free

Western Blot: PTRH2 AntibodyBSA Free [NB100-55963]

Western Blot: PTRH2 AntibodyBSA Free [NB100-55963]

Western Blot: PTRH2 Antibody [NB100-55963] - Western blot analysis of Bit1 in Daudi cell lysate with Bit1 antibody using NB100-55963 at (A) 1, (B) 2, and (C) 4 ug/ml.
PTRH2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PTRH2 Antibody - BSA Free [NB100-55963] -

Immunocytochemistry/ Immunofluorescence: PTRH2 Antibody - BSA Free [NB100-55963] - Immunofluorescence of PTRH2 in Human Small Intestine cells with PTRH2 antibody at 20 ug/mL.
PTRH2 Antibody - BSA Free

Immunohistochemistry: PTRH2 Antibody - BSA Free [NB100-55963] -

Immunohistochemistry: PTRH2 Antibody - BSA Free [NB100-55963] - Immunohistochemistry of PTRH2 in human small intestine tissue with PTRH2 antibody at 10 ug/mL.

Applications for PTRH2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

10 ug/mL

Western Blot

1-4 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PTRH2

Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process. Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE. Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha 5/ beta 1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha 5/ beta 1 integrin.

Alternate Names

Bcl-2 inhibitor of transcription 1, BIT1CGI-147, EC 3.1.1.29, mitochondrial, peptidyl-tRNA hydrolase 2, PTH2FLJ32471

Entrez Gene IDs

51651 (Human)

Gene Symbol

PTRH2

Additional PTRH2 Products

Product Documents for PTRH2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PTRH2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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