PU.1/Spi-1 Antibody (PU1/2146)

Name: PU.1/Spi-1 Antibody (PU1/2146)
Brand: Novus Biologicals
SKU: NBP2-75738
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-75738

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # PU1/2146

View all PU.1/Spi-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 16-170) of human PU.1/Spi-1 protein (Exact sequence is proprietary) (Uniprot: P17947 )

Localization

Nuclear

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-75766)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for PU.1/Spi-1 Antibody (PU1/2146)

Western Blot: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738]

Western Blot: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Analysis of THP-1 cell lysate using PU.1 Mouse Monoclonal Antibody (PU1/2146).

Immunocytochemistry/ Immunofluorescence: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738]

Immunocytochemistry/Immunofluorescence: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Immunofluorescence Analysis of PFA-fixed Ramos cells labeling PU.1 with PU.1/Spi-1 Antibody (PU1/2146) followed by Goat anti-Mouse IgG-CF488 (Green).
Membrane is labeled with Phalloidin (Red).

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738]

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Spleen stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).

Flow Cytometry: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738]

Flow Cytometry: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Flow Cytometric Analysis of PFA-fixed Ramos cells. PU.1/Spi-1 Antibody (PU1/2146) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738]

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738]

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738]

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Lymph Node stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).

Protein Array: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738]

Protein Array: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PU.1/Spi-1 Antibody (PU1/2146)Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

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Applications for PU.1/Spi-1 Antibody (PU1/2146)

Application

Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml

Application Notes

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: PU.1/Spi-1

PU.1/Spi1 encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Hematopoietic transcription factor PU.1

Alternate Names

OF, SFPI1, Spi-1, SPI-A, SPI1

Gene Symbol

SPI1

UniProt

P17947 (Human)

Additional PU.1/Spi-1 Products

Product Documents for PU.1/Spi-1 Antibody (PU1/2146)

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Certificate of Analysis

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Product Specific Notices for PU.1/Spi-1 Antibody (PU1/2146)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.