PU.1/Spi-1 Antibody (rPU1/2146)

Name: PU.1/Spi-1 Antibody (rPU1/2146)
Brand: Novus Biologicals
SKU: NBP3-48984
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-48984

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG₁ kappa Clone # rPU1/2146 expressed in CHO

View all PU.1/Spi-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 16-170) of human PU.1 protein (exact sequence is proprietary) (Uniprot: P17947)

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Scientific Data Images for PU.1/Spi-1 Antibody (rPU1/2146)

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (rPU1/2146) [NBP3-48984] -

Formalin-fixed, paraffin-embedded human lymph node stained with PU.1/Spi-1 Antibody (rPU1/2146).

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (rPU1/2146) [NBP3-48984] -

Formalin-fixed, paraffin-embedded human lymph node stained with PU.1/Spi-1 Antibody (rPU1/2146).

Applications for PU.1/Spi-1 Antibody (rPU1/2146)

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: PU.1/Spi-1

PU.1/Spi1 encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Hematopoietic transcription factor PU.1

Alternate Names

OF, SFPI1, Spi-1, SPI-A, SPI1

Gene Symbol

SPI1

Additional PU.1/Spi-1 Products

Product Documents for PU.1/Spi-1 Antibody (rPU1/2146)

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PU.1/Spi-1 Antibody (rPU1/2146)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.