![Western Blot: PU.1/Spi-1 Antibody [NBP1-00135]](https://resources.rndsystems.com/images/products/PU.1-Spi-1-Antibody-Western-Blot-NBP1-00135-img0005.jpg "Western Blot: PU.1/Spi-1 Antibody [NBP1-00135]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence C-DLYQRQTHEYY corresponding to internal region according to NP_001074016.1, NP_003111.2.
Epitope
internal region
Specificity
This antibody is expected to recognise both reported isoforms (NP_001074016.1; NP_003111.2).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for PU.1/Spi-1 Antibody
Western Blot: PU.1/Spi-1 Antibody [NBP1-00135]
Western Blot: PU.1/Spi-1 Antibody [NBP1-00135] - (0.03ug/ml) Analysis of DAUDI lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP1-00135]](https://resources.rndsystems.com/images/products/PU.1-Spi-1-Antibody-Immunohistochemistry-Paraffin-NBP1-00135-img0007.jpg "Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP1-00135]")
Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP1-00135]
Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP1-00135] - (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.![Flow Cytometry: PU.1/Spi-1 Antibody [NBP1-00135]](https://resources.rndsystems.com/images/products/PU.1-Spi-1-Antibody-Flow-Cytometry-NBP1-00135-img0006.jpg "Flow Cytometry: PU.1/Spi-1 Antibody [NBP1-00135]")
Flow Cytometry: PU.1/Spi-1 Antibody [NBP1-00135]
Flow Cytometry: PU.1/Spi-1 Antibody [NBP1-00135] - Analysis of PU.1/Spi1 in p3xFLAG-PU.1 transfected 293T cells (1 million cells) using anti-PU.1/Spi1 antibody. Image from verified customer review.Applications for PU.1/Spi-1 Antibody
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Peptide ELISA
Detection limit 1:32000
Western Blot
0.03 ug/ml
Application Notes
WB: Approx. 40 kDa band observed in lysates of DAUDI and K562 is reported in scientific literature (PMID: 10867017).
Reviewed Applications
Read 2 reviews rated 4 using NBP1-00135 in the following applications:
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PU.1/Spi-1
Long Name
Hematopoietic transcription factor PU.1
Alternate Names
OF, SFPI1, Spi-1, SPI-A, SPI1
Gene Symbol
SPI1
UniProt
Additional PU.1/Spi-1 Products
Product Documents for PU.1/Spi-1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PU.1/Spi-1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for PU.1/Spi-1 Antibody
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Customer Reviews for PU.1/Spi-1 Antibody (2)
4 out of 5
2 Customer Ratings
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Customer Images
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Application: Flow CytometrySample Tested: Control 293T cell and cells transfected with p3XFLAG-PU.1Species: HumanVerified Customer | Posted 10/16/2014FACS for PU.1 in p3XFLAG-PU.1 transfected 293T cells
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Application: Western BlotSample Tested: 293T lysateSpecies: HumanVerified Customer | Posted 10/16/2014PU.1 Wb in 293T cells
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
