PU.1/Spi-1 Antibody - BSA Free

Name: PU.1/Spi-1 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP2-33427
Price: 559 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-33427

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

View all PU.1/Spi-1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: EDLVPYDTDLYQRQTHEYYPYLSSDGESHSDHYWDFHPHHVHSEFESFAENNFTELQSVQPPQLQQLYRHMELEQMHVLDTPMVPPHPSLGHQVSYLPRMCLQYPSLSPAQPSSDEEEG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PU.1/Spi-1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PU.1/Spi-1 Antibody [NBP2-33427]

Immunocytochemistry/Immunofluorescence: PU.1/Spi-1 Antibody [NBP2-33427] - Staining of human cell line REH shows localization to nucleoplasm. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP2-33427]

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP2-33427] - Staining in human bone marrow and pancreas tissues using anti-SPI1 antibody. Corresponding SPI1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP2-33427]

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP2-33427] - Staining of human bone marrow shows high expression.

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP2-33427]

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP2-33427] - Staining of human pancreas shows low expression as expected.

Chromatin Immunoprecipitation-exo-Seq: PU.1/Spi-1 Antibody - BSA Free [NBP2-33427]

ChIP-Exo-Seq composite graph for Anti-SPI1 (NBP2-33427) tested in GM12878 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

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Applications for PU.1/Spi-1 Antibody - BSA Free

Application

Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PU.1/Spi-1

PU.1/Spi1 encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Hematopoietic transcription factor PU.1

Alternate Names

OF, SFPI1, Spi-1, SPI-A, SPI1

Gene Symbol

SPI1

UniProt

P17947 (Human)

Additional PU.1/Spi-1 Products

Product Documents for PU.1/Spi-1 Antibody - BSA Free

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Product Specific Notices for PU.1/Spi-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.