PUMA Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76639

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PUMA Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human PUMA isoform 1. The immunogen is located within the first 50 amino acids of PUMA. Amino Acid Squence: ARARQEGSSPEPVEG

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 16399862).

Specificity

Human PUMA has 4 isoforms, including isoform 1 (193aa, 21kD), isoform 2 (131aa, 14kD), isoform 3 (101aa, 10kD) and isofrom 4 (261aa, 27kD). This antibody detects human isoform 1, and is predicted to detect mouse and rat PUMA (193aa, 21kD for both of them).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PUMA Antibody - BSA Free

Western Blot: PUMA AntibodyBSA Free [NBP1-76639]

Western Blot: PUMA AntibodyBSA Free [NBP1-76639]

PUMA-Antibody-Western-Blot-NBP1-76639-img0011.jpg
Western Blot: PUMA AntibodyBSA Free [NBP1-76639]

Western Blot: PUMA AntibodyBSA Free [NBP1-76639]

Western Blot: PUMA Antibody - BSA Free [NBP1-76639] - PUMA Antibody [NBP1-76639] - K562 cell lysate with PUMA antibody at 2 ug/ml.
Western Blot: PUMA AntibodyBSA Free [NBP1-76639]

Western Blot: PUMA AntibodyBSA Free [NBP1-76639]

Western Blot: PUMA Antibody - BSA Free [NBP1-76639] - PUMA Antibody [NBP1-76639] - Independent Antibody Validation (IAV) via Protein Expression ProfileLoading: 20 ug of lysates per lane.Antibodies: NBP1-76654 (3 ug/mL), NBP1-76639 (2 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.
PUMA Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PUMA Antibody - BSA Free [NBP1-76639] -

Immunocytochemistry/ Immunofluorescence: PUMA Antibody - BSA Free [NBP1-76639] - Validation of PUMA in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling PUMA with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
PUMA Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PUMA Antibody - BSA Free [NBP1-76639] -

Immunocytochemistry/ Immunofluorescence: PUMA Antibody - BSA Free [NBP1-76639] - Validation of PUMA in K562. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling PUMA with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing cytosol staining on K562 cells
PUMA Antibody - BSA Free

Immunohistochemistry: PUMA Antibody - BSA Free [NBP1-76639] -

Immunohistochemistry: PUMA Antibody - BSA Free [NBP1-76639] - Validation of PUMA in Human Breast Carcinoma. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PUMA antibody at 10 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
PUMA Antibody - BSA Free

Immunohistochemistry: PUMA Antibody - BSA Free [NBP1-76639] -

Immunohistochemistry: PUMA Antibody - BSA Free [NBP1-76639] - Validation of PUMA in Human Breast Tissue. Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-PUMA antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Applications for PUMA Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/mL

Immunohistochemistry

2.5-10 ug/mL

Western Blot

2-3 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PUMA

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Alternate Names

BCL2 binding component 3, bcl-2-binding component 3, JFY1, JFY-1, p53 up-regulated modulator of apoptosis, PUMAFLJ42994

Gene Symbol

BBC3

UniProt

NP_055232 (Human)

Additional PUMA Products

Product Documents for PUMA Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PUMA Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PUMA Antibody - BSA Free

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Protocols

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