Purmorphamine

Tocris Bioscience | Catalog # 4551

Smo receptor agonist
Tocris Bioscience

Key Product Details

Description

Smo receptor agonist
View all Smoothened Receptor Agonists »

Product Description

Purmorphamine is a smoothened (Smo) receptor agonist (EC50 ~ 1 μM) and activates the Hedgehog pathway. Purmorphamine induces osteogenesis in mouse mesenchymal progenitor cells (C3H10T1/2). When combined with BMP-4, Purmorphamine can transdifferentiate pre-adipocytes (3T3-L1) and myoblasts (C2C12) into osteoblasts. Purmorphamine induces differentiation of multipotent mesenchymal progenitor cells into osteoblasts, and of spinal motor neurons from pluripotent human stem cells, and protects dopaminergic neurons in a mouse model of Parkinson's disease.

Scientific Data Images for Purmorphamine

applications of purmorphamine in neurons derived from hiPSCs

Applications of Purmorphamine in Neurons Derived from hiPSCs

Dopaminergic neurons derived from human induced pluripotent stem cells (hiPSC) using SB 431542 (Catalog # 1614, Tocris), CHIR 99021 (Catalog # 4423, Tocris), DAPT (Catalog # 2634, Tocris) and Purmorphamine. Cells cultured in Neuronal Media supplemented with TGF-3 (Catalog # 8420-B3, R&D Systems), cAMP, GDNF (Catalog # 212-GD, R&D Systems), BDNF (Catalog # 248-BDB, R&D Systems), N21-MAX (Catalog # AR008, R&D Systems). Differentiation shown at days 55 (left) and 62 (right). Images courtesy of Kevin Flynn, Bio-Techne.

Product Specifications for Purmorphamine

Molecular Weight

520.62

Formula

C31H32N6O2

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

9-Cyclohexyl-N-[4-(4-morpholinyl)phenyl]-2-(1-naphthalenyloxy)-9H-purin-6-amine

CAS Number

483367-10-8

PubChem ID

5284329

InChI Key

FYBHCRQFSFYWPY-UHFFFAOYSA-N

SMILES

C1(OC4=CC=CC5=C4C=CC=C5)=NC(NC3=CC=C(N6CCOCC6)C=C3)=C2C(N(C7CCCCC7)C=N2)=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 52.06 100

Preparing Stock Solutions for Purmorphamine

The following data is based on the product molecular weight 520.62.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.92 mL 9.60 mL 19.21 mL
5 mM 0.38 mL 1.92 mL 3.84 mL
10 mM 0.19 mL 0.96 mL 1.92 mL
50 mM 0.04 mL 0.19 mL 0.38 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 131 publications citing the usage of this product.

Product Documents for Purmorphamine

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Purmorphamine

For research use only

Customer Reviews for Purmorphamine (3)

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  • Usage Information
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: iPSC
    Verified Customer | Posted 07/04/2020
    From Day 2 to day 9 to direct the cells toward ventral diencephalon with regular media change every 2 days.
    iPSC-derived hypothalamic neuron differentiation activation by Smoothened agonist SAG 1 μM and purmorphamine 1 μM.
    Purmorphamine 4551
  • For differentiation of DaNs
    Name: Phi Anh Phan
    Species: Human
    Cell Line/Tissue: iPSC
    Verified Customer | Posted 02/04/2020
    Used for the differentiation of human iPSCs into dopaminergic neurons (Kriks et al.,2011).
    Purmorphamine 4551
  • Great for differentiation of huiPSCs into DaNs!
    Name: Phi Anh Phan
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: iPSC
    Verified Customer | Posted 01/31/2020
    Purmorphamine was used to differentiate human iPSCs into dopaminergic neurons with above 60% TH positive cells.
    Purmorphamine 4551

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