Chemical Name: 9-Cyclohexyl-N-[4-(4-morpholinyl)phenyl]-2-(1-naphthalenyloxy)-9H-purin-6-amine
Biological ActivityPurmorphamine is a smoothened (Smo) receptor agonist (EC50 ~ 1 μM) and activates the Hedgehog pathway. Purmorphamine induces osteogenesis in mouse mesenchymal progenitor cells (C3H10T1/2). When combined with BMP-4, Purmorphamine can transdifferentiate pre-adipocytes (3T3-L1) and myoblasts (C2C12) into osteoblasts. Purmorphamine induces differentiation of multipotent mesenchymal progenitor cells into osteoblasts, and of spinal motor neurons from pluripotent human stem cells, and protects dopaminergic neurons in a mouse model of Parkinson's disease.
Applications of Purmorphamine in Neurons Derived from hiPSCs Dopaminergic neurons derived from human induced pluripotent stem cells (hiPSC) using SB 431542 (Catalog # 1614, Tocris), CHIR 99021 (Catalog # 4423, Tocris), DAPT (Catalog # 2634, Tocris) and Purmorphamine. Cells cultured in Neuronal Media supplemented with TGF-3 (Catalog # 8420-B3, R&D Systems), cAMP, GDNF (Catalog # 212-GD, R&D Systems), BDNF (Catalog # 248-BDB, R&D Systems), N21-MAX (Catalog # AR008, R&D Systems). Differentiation shown at days 55 (left) and 62 (right). Images courtesy of Kevin Flynn, Bio-Techne.
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Purmorphamine induces osteogenesis by activation of the hedgehog signaling pathway.
Wu et al.
Purmorphamine activates the Hedgehog pathway by targeting Smoothened.
Sinha et al.
A small molecule with osteogenesis-inducing activity in multipotent mesenchymal progenitor cells.
Wu et al.
Citations for Purmorphamine
The citations listed below are publications that use Tocris products. Selected citations for Purmorphamine include:
22 Citations: Showing 1 - 10
Generation and characterization of NGLY1 patient-derived midbrain organoids.
Authors: Wei Et al.
Front Cell Dev Biol 2023;11:1039182
Dynamic 3D Combinatorial Generation of hPSC-Derived Neuromesodermal Organoids With Diverse Regional and Cellular Identities.
Authors: Mustafa Et al.
Curr Protoc 2022;2:e568
Directed differentiation of human pluripotent stem cells into diverse organ-specific mesenchyme of the digestive and respiratory systems.
Authors: Lu Et al.
Nat Protoc 2022;17:2699-2719
Primary cilia and SHH signaling impairments in human and mouse models of Parkinson's disease.
Authors: Beate Et al.
Nat Commun 2022;13:4819
Genome-wide identification of the genetic basis of amyotrophic lateral sclerosis.
Authors: Cheng Et al.
ARP-T1-associated Bazex-Dupré-Christol syndrome is an inherited basal cell cancer with ciliary defects characteristic of ciliopathies.
Authors: Fanny Et al.
Commun Biol 2021;4:544
Growth factor mimetics for skin regeneration: In vitro profiling of primary human fibroblasts and keratinocytes.
Authors: Willeke F Et al.
Arch Pharm (Weinheim) 2021;354:e2100082
Human Pluripotent Stem Cells for High-Throughput Drug Screening and Characterization of Small Molecules.
Authors: Ruili Et al.
Methods Mol Biol 2021;2454:811-827
Robust single-cell discovery of RNA targets of RNA-binding proteins and ribosomes.
Authors: Jason G Et al.
Nat Methods 2021;18:507-519
Variable Outcomes in Neural Differentiation of Human PSCs Arise from Intrinsic Differences in Developmental Signaling Pathways.
Authors: Frederick J Et al.
Cell Rep 2020;31:107732
Transcriptomic and epigenomic dynamics associated with development of human iPSC-derived GABAergic interneurons.
Authors: Ying Et al.
Hum Mol Genet 2020;29:2579-2595
IPSC-Derived Neuronal Cultures Carrying the Alzheimer's Disease Associated TREM2 R47H Variant Enables the Construction of an Aβ-Induced Gene Regulatory Network.
Authors: Kristel Et al.
Int J Mol Sci 2020;21
Single-Cell Sequencing of iPSC-DA Neurons Reconstructs Disease Progression and Identifies HDAC4 as a Regulator of Parkinson Cell Phenotypes.
Authors: Lang Et al.
Cell Stem Cell 2019;24:93
Generating Human iPSC-Derived Astrocytes with Chemically Defined Medium for In Vitro Disease Modeling.
Authors: Georg C Et al.
Methods Mol Biol 2019;1994:31-39
FGF Modulates the Axial Identity of Trunk hPSC-Derived Neural Crest but Not the Cranial-Trunk Decision.
Authors: Hackland Et al.
Stem Cell Reports 2019;12:920
IFT80 is required for stem cell proliferation, differentiation, and odontoblast polarization during tooth development.
Authors: Yuan Et al.
Cell Death Dis 2019;10:63
A stably self-renewing adult blood-derived induced neural stem cell exhibiting patternability and epigenetic rejuvenation.
Authors: Sheng Et al.
Nat Commun 2018;9:4047
Th17 lymphocytes induce neuronal cell death in a human iPSC-based model of Parkinson's disease.
Authors: Sommer Et al.
Cell Stem Cell. 2018;23:123
Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone Responses.
Authors: Rajamani Et al.
Cell Stem Cell 2018;22:698
Zooming in on Cryopreservation of hiPSCs and Neural Derivatives: A Dual-Center Study Using Adherent Vitrification.
Authors: Kaindl Et al.
Stem Cells Transl Med 2018;
Ciliary IFT80 balances canonical versus non-canonical hedgehog signalling for osteoblast differentiation.
Authors: Yuan Et al.
J Immunol 2016;7:11024
Characterization of axon formation in the embryonic stem cell-derived motoneuron.
Authors: Shinn-Zong Et al.
Cell Transplant 2010;20:493-502
No product specific FAQs exist for this product, however you mayView all Small Molecule FAQs
VersaClone cDNA Plasmids
Reviews for Purmorphamine
There are currently no reviews for this product. Be the first to review Purmorphamine and earn rewards!
Have you used Purmorphamine?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image