SB 431542
Tocris Bioscience | Catalog # 1614
Key Product Details
Description
Product Description
SB 431542 is a potent and selective inhibitor of the transforming growth factor-β (TGF-β) type I receptor/ALK5 (IC50 = 94 nM), and its relatives ALK4 and ALK7. Suppresses TGF-β-induced proliferation of human osteosarcoma cells. Replaces SOX2 in reprogramming of fibroblasts into iPSCs. Stimulates proliferation, differentiation and sheet formation of ESC-derived endothelial cells. Inhibits TGF-β-induced EMT, migration, invasion and VEGF secretion in several human cancer cell lines. Also used in a protocol to generate brain organoids from human iPSCs. Can be used in a small molecule cocktail to generate 3D culture of lung alveolar cells, or vascular organoids. Also used in a protocol to generate cortical neurons from hiPSCs or dopaminergic neurons from hPSCs (see our protocol below).
SB 431542 synthesized to cGMP guidelines also available.
For more information about how SB 431542 may be used, see our protocols: 3D Culture of Lung Alveolar Cells, Differentiation of Hematopoietic Progenitors from hPSCs, Generating Midbrain Dopaminergic Neurons from hPSCs, Generating Vascular Organoids, Accelerated Induction of Cortical Neurons from hiPSCs.
Scientific Data Images for SB 431542
Application of SB 431542 in dopaminergic neurons derived from hiPSCs.
Dopaminergic neurons derived from human induced pluripotent stem cells (hiPSC) using SB 431542, CHIR 99021 (Catalog # 4423, Tocris), DAPT (Catalog # 2634, Tocris) and Purmorphamine (Catalog # 4551, Tocris). Cells cultured in Neuronal Media supplemented with TGF-3 (Catalog # 8420-B3, R&D Systems), cAMP, GDNF (Catalog # 212-GD, R&D Systems), BDNF (Catalog # 248-BDB, R&D Systems), N21-MAX (Catalog # AR008, R&D Systems). Differentiation shown at days 55 (left) and 62 (right). Images courtesy of Kevin Flynn, Bio-Techne.
Product Specifications for SB 431542
Molecular Weight
Formula
Storage
Purity
Chemical Name
CAS Number
PubChem ID
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 38.44 | 100 with gentle warming | |
| Ethanol | 3.84 | 10 |
Preparing Stock Solutions for SB 431542
The following data is based on the product molecular weight 384.39.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.60 mL | 13.01 mL | 26.02 mL |
| 5 mM | 0.52 mL | 2.60 mL | 5.20 mL |
| 10 mM | 0.26 mL | 1.30 mL | 2.60 mL |
| 50 mM | 0.05 mL | 0.26 mL | 0.52 mL |
Calculators
Background References
References are publications that support the biological activity of the product. See our Citations tab to view 2341 publications citing the usage of this product.
- Schafer An in vivo neuroimmune organoid model to study human microglia phenotypes. Cell 2023 PMID: 37172564
- Halder A specific inhibitor of TGF-beta receptor kinase, SB-431542, as a potent antitumor agent for human cancers. Neoplasia 2005 PMID: 15967103
- Watabe TGF-beta receptor kinase inhibitor enhances growth and integrity of embryonic stem cell-derived endothelial cells. J.Cell Biol. 2003 PMID: 14676305
- Matsuyama SB-431542 and Gle. inhibit transforming growth factor-β-induced proliferation of human osteosarcoma cells. Cancer Res. 2003 PMID: 14633705
- Laping Inhibition of transforming growth factor (TGF)-β1-induced extracellular matrix with a novel inhibitor of the TGF-β type I receptor kinase activity: SB-431542. Mol.Pharmacol. 2002 PMID: 12065755
- Inman SB-431542 is a potent and specific inhibitor of transforming growth factor-β superfamily type I AVNreceptor-like kinase (ALK) receptors ALK4, ALK5, and ALK7. Mol.Pharmacol. 2002 PMID: 12065756
Product Documents for SB 431542
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SB 431542
For research use only
Related Research Areas
Citations for SB 431542
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Customer Reviews for SB 431542 (13)
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Species: MouseAssay Type: In VitroCell Line/Tissue: HL-1 cell lineVerified Customer | Posted 12/20/2024SB431542 was used as an inhibitor for TGF-beta. Briefly, the cells were knockdown for AMPK family members and treated with the inhibitor for 24 hrs at 1 micromolar concentration.
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Species: HumanAssay Type: In VitroCell Line/Tissue: colorectal cancer organoidsVerified Customer | Posted 08/14/2023I used the inhibitor for growing colorectal cancer organoids. Concentration is at 10 uM.
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Species: MouseAssay Type: In VitroCell Line/Tissue: primary cellVerified Customer | Posted 05/23/2023using for organoid culture. (lot#16A/281017)
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Species: HumanAssay Type: In VitroCell Line/Tissue: hESCs/Tc28a2sVerified Customer | Posted 12/11/2020If possible perform titration of SB431542 with TGFb signalling output depending upon cell type or application.I have used SB431542 for both selective inhibition of TGFb family receptors in TGFb activity assays and to drive differentiation of pluripotent stem cells towards mesodermal tissues. Concentration: 1nM working
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Species: HumanAssay Type: In VitroCell Line/Tissue: Induced pluripotent stem cellsVerified Customer | Posted 12/11/2020Keep at -80C for a maximum of 1 monthI routinely use this product to differentiate human induced pluripotent stem cells into cortical neurons. I use it at 10uM. Once reconstituted, I would only store it for a maximum of one month in -80C
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Species: HumanAssay Type: In VitroCell Line/Tissue: A549Verified Customer | Posted 10/26/2020Tested a human lung epithelial cell line pretreated with SB431542 at either 10, 1 or 0.1uM for 30 min and then added TGFb1 at 5ng/ml in two different matrices. Incubated 24hr and then lysed cells to look at pSmad 2/3 levels by immunoassay
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Species: HumanAssay Type: In VitroCell Line/Tissue: iPSCVerified Customer | Posted 10/13/2020iPSC culture medium with 4 uM SB431542Differentiation of human iPSCs into hematopoietic progenitor cells
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Species: HumanAssay Type: In VitroCell Line/Tissue: Human PSCVerified Customer | Posted 06/30/2020Make 100µl aliquots and keep them at −20 °C for up to 6 months.For hPSC culture, final concentration 10 μM.
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Species: HumanAssay Type: In VitroCell Line/Tissue: iPS cellsVerified Customer | Posted 02/06/2020Worked consistently in generation of cerebral organoids from human iPS cells
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Species: HumanAssay Type: In VitroCell Line/Tissue: iPSCVerified Customer | Posted 01/15/2020Human induced pluripotent stem cells (iPSCs) were differentiated into neural progenitor cells (NPCs) as described in Chambers et al. 2009 (PMID: 19252484.) SB431542 was used at 20uM. Differentiated NPCs were then immunostained using PAX6 monoclonal Ab, and we had over 80% of PAX6 positive cells.
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Species: HumanAssay Type: In VitroCell Line/Tissue: Breast Cancer CellsVerified Customer | Posted 12/08/2019I used it as a TGF-beta receptor Inhibitor in Breast cancer Cells to study the impact of blocking TGF-beta pathway on EMT. I used the concentration 5uM and it works really well.
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Species: HumanAssay Type: In VitroCell Line/Tissue: stem cells differentiated endothelial cellsVerified Customer | Posted 05/01/2018Stems cells undergoing differentiation to endothelial cells utilize addition of SB 431542. FLK+ve cells cultured with SB 431542 revealed colonies of endothelial cells (arrows)
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Species: HumanAssay Type: In VitroCell Line/Tissue: Induced pluripotent stem cellsVerified Customer | Posted 01/04/2018I used this product for differentiation of iPSCs into dopaminergic neurons according to the protocol published in Kriks et al, 2011. I used the concentration 10uM and it works really well.
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