Recombinant Human TGF-beta 3 (CHO-expressed) Protein

Carrier Free

Catalog # Availability Size / Price Qty
8420-B3-005/CF
8420-B3-025/CF
8420-B3-100/CF

With Carrier

Catalog # Availability Size / Price Qty
8420-B3-005
8420-B3-025
Recombinant Human TGF-beta 3 (CHO-expressed) Protein Bioactivity
1 Image
Product Details
Citations (3)
FAQs
Reviews (2)

Recombinant Human TGF-beta 3 (CHO-expressed) Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.01-0.04 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human TGF-beta 3 protein
Ala301-Ser412
Accession #
N-terminal Sequence
Analysis
Ala301
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
13 kDa
SDS-PAGE
9-11 kDa, reducing conditions

Product Datasheets

8420-B3 (with carrier)

8420-B3/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8420-B3

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8420-B3/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 50 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human TGF-beta 3 (CHO-expressed) Protein Bioactivity View Larger

Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. The ED50 for this effect is 0.01-0.04 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta 3

TGF­-beta 3 (transforming growth factor-beta 3) is a member of a TGF­-beta superfamily subgroup that is defined by their structural and functional similarities (1-5). TGF-beta 3 and its closely related proteins, TGF-beta 1 and ­ beta 2, act as cellular switches to regulate immune function, cell proliferation, and epithelial-­mesenchymal transition (4, 6, 7). The non-redundant biological effects of TGF-­ beta 3 include involvement in palatogenesis, chondrogenesis, and pulmonary development (1, 2, 7-9). Human TGF­-beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein. The proprotein is processed by a furin-­like convertase to generate a 220 aa latency-­associated peptide (LAP) and a 112 aa mature TGF­-beta 3 (10, 11). Mature human TGF-­ beta 3 shows 100%, 99%, and 98% aa identity with mouse/dog/horse, rat, and pig TGF-­ beta 3, respectively. TGF-beta 3 is secreted as a latent complex. This latent form of TGF-beta 3 is activated by integrins, thrombospondin-1, plasmin, and matrix metalloproteases (12, 13). It can also be activated by extreme pH and reactive oxygen species (1-5, 12). TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF-­ beta RI or ALK-­1, to modulate transcription through Smad phosphorylation (14-16). The divergent biological effects exerted by individual TGF-beta isoforms is dependent upon the recruitment of co-receptors (TGF-­ beta RIII and endoglin) and the subsequent initiation of Smad­-dependent or -independent signaling pathways (15, 17, 18).

References
  1. Barrio, M.C. et al. (2014) Cells Tissues Organs. [Epub ahead of print; PMID 24861080].
  2. Doetschman, T. et al. (2012) Genesis 50:59.
  3. Mittl, P.R. et al. (1996) Protein Sci. 5:1261.
  4. Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
  5. Wahl, S.M. et al. (2006) Immunol. Rev. 213:213.
  6. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  7. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  8. Jin, J.Z. et al. (2014) Dev. Dyn. [Epub ahead of print; PMID 25104574].
  9. Tang, Q.O. et al. (2009) Expert Opin. Biol Ther. 9:689.
  10. Derynck, R. et al. (1988) EMBO J. 7:3737.
  11. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  12. Munger, J.S. et al. (1997) Kidney Int 51:1376.
  13. Wipff, P.J. and B. Hinz (2008) Eur J Cell Biol 87:601.
  14. Cui, X.M. and C.F. Shuler (2000) Int. J. Dev. Biol. 44:397.
  15. de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
  16. Nakajima, A. et al. (2007) Dev. Dyn. 236:791.
  17. Iwata, J. et al. (2012) J. Clin. Invest. 122:873.
  18. Gatza, C.E. et al. (2010) Cell. Signal. 22:1163.
Long Name
Transforming Growth Factor beta 3
Entrez Gene IDs
7043 (Human); 21809 (Mouse); 25717 (Rat)
Alternate Names
ARVD; ARVD1; FLJ16571; LDS5; RNHF; TGFB3; TGFbeta 3; TGF-beta 3; TGF-beta3; TGF-beta-3; transforming growth factor beta-3; transforming growth factor, beta 3

Citations for Recombinant Human TGF-beta 3 (CHO-expressed) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Transforming Growth Factor Beta 3-Loaded Decellularized Equine Tendon Matrix for Orthopedic Tissue Engineering
    Authors: SP Roth, W Brehm, C Gro beta, P Scheibe, S Schubert, J Burk
    Int J Mol Sci, 2019;20(21):.
    Species: Equine
    Sample Types: Whole Tissue
    Applications: Cell Culture
  2. Cancer-associated fibroblast-derived Gremlin 1 promotes breast cancer progression
    Authors: J Ren, M Smid, J Iaria, DCF Salvatori, H van Dam, HJ Zhu, JWM Martens, P Ten Dijke
    Breast Cancer Res., 2019;21(1):109.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Growth Factor-Mediated Tenogenic Induction of Multipotent Mesenchymal Stromal Cells Is Altered by the Microenvironment of Tendon Matrix
    Authors: SP Roth, S Schubert, P Scheibe, C Gro beta, W Brehm, J Burk
    Cell Transplant, 2018;0(0):9636897187922.
    Species: Equine
    Sample Types: Whole Tissue
    Applications: Bioassay

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Reviews for Recombinant Human TGF-beta 3 (CHO-expressed) Protein

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Recombinant Human TGF-beta 3 (CHO-expressed) Protein
By Anonymous on 03/16/2020
Application: Binding assay/Protein-protein interaction

TGFb3 binds TBRII in an ELISA assay. TBRII is immobilized on the plate and dose response of TGFb3 interaction is detected by anti-TGFb3.


Recombinant Human TGF-beta 3 (CHO-expressed) Protein
By Anonymous on 05/07/2019
Application: CellProlif