DAPT

Catalog # Availability Size / Price Qty
2634/10
2634/50
DAPT | CAS No. 208255-80-5 | Secretase Inhibitors
1 Image
Description: Gamma γ-secretase inhibitor; induces neuronal differentiation; blocks Notch signaling

Chemical Name: (2S)-N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine 1,1-dimethylethyl ester

Purity: ≥99%

Product Details
Citations (51)
Reviews (6)

Biological Activity

DAPT is a γ-secretase inhibitor. DAPT reduces Aβ40 and Aβ42 levels in human primary neuronal cultures (IC50 values are 115 and 200 nM for total Aβ and Aβ42 respectively) and in brain extract, cerebrospinal fluid and plasma in vivo. DAPT has no effect on APPα and APPβ levels. DAPT blocks Notch signaling in hybrid human-mouse fetal thymus organ culture (FTOC) and causes ESCs to commit to neuronal differentiation. DAPT can be used in a small molecule cocktail to derive cortical neurons from hPSCs and to maintain hepatocytes in culture. DAPT also promotes the formation of cone photoreceptors in retinal organoids.

Applications in hiPSCs

 Dopaminergic Neurons Derived from hiPSCs using DAPT

Dopaminergic Neurons Derived from hiPSCs using DAPT

Dopaminergic neurons derived from human induced pluripotent stem cells (hiPSC) using SB 431542 (Cat. No. 1614), CHIR 99021 (Cat. No. 4423), DAPT and Purmorphamine (Cat. No. 4551). Cells cultured in Neuronal Media supplemented with TGF-3 (Cat. No. 8420-B3, R&D Systems), cAMP, GDNF (Cat. No. 212-GD, R&D Systems), BDNF (Cat. No. 248-BDB, R&D Systems), N21-MAX (Cat. No. AR008, R&D Systems). Differentiation shown at days 55 (left) and 62 (right). Images courtesy of Kevin Flynn, Bio-Techne.

Technical Data

M.Wt:
432.46
Formula:
C23H26F2N2O4
Solubility:
Soluble to 100 mM in DMSO
Purity:
≥99%
Storage:
Store at +4°C
CAS No:
208255-80-5

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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Citations for DAPT

The citations listed below are publications that use Tocris products. Selected citations for DAPT include:

51 Citations: Showing 1 - 10

  1. A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids
    Authors: Yang Et al.
    Cell Stem Cell  2020;27:125
  2. Quantification of human neuromuscular function through optogenetics.
    Authors: Vila Et al.
    Theranostics  2019;9:1232
  3. Long-term functional maintenance of primary human hepatocytes in vitro.
    Authors: Xiang Et al.
    Science  2019;364:399
  4. Neuropathy-causing mutations in HSPB1 impair autophagy by disturbing the formation of SQSTM1/p62 bodies.
    Authors: Haidar Et al.
    Autophagy  2019;15:1051
  5. HSP90-incorporating chaperome networks as biosensor for disease-related pathways in patient-specific midbrain DA neurons.
    Authors: Kishinevsky Et al.
    Nat Commun  2018;9:4345
  6. A stably self-renewing adult blood-derived induced neural stem cell exhibiting patternability and epigenetic rejuvenation.
    Authors: Sheng Et al.
    Nat Commun  2018;9:4047
  7. A biodegradable hybrid inorganic nanoscaffold for advanced stem cell therapy.
    Authors: Yang
    Nat Commun  2018;9:3147
  8. Co-targeting of Tiam1/Rac1 and Notch ameliorates chemoresistance against dox. in a biomimetic 3D lymphoma model.
    Authors: Ikram Et al.
    Oncotarget  2018;9:2058
  9. VGF nerve growth factor inducible is involved in retinal ganglion cells death induced by optic nerve crush.
    Authors: Takeuchi Et al.
    Sci Rep  2018;8:16443
  10. Gain of toxic apolipoprotein E4 effects in human iPSC-derived neurons is ameliorated by a small-molecule structure corrector.
    Authors: Wang
    Nat Med  2018;24(5):647
  11. Hey2 regulates the size of the cardiac progenitor pool during vertebrate heart development.
    Authors: Gibb Et al.
    Development  2018;145
  12. The COPII cargo adapter SEC24C is essential for neuronal homeostasis.
    Authors: Wang Et al.
    J Clin Invest  2018;128:3319
  13. ErbB4 signaling stimulates pro-inflammatory macrophage apoptosis and limits colonic inflammation.
    Authors: Schumacher
    Cell Death Dis  2017;8:e2622
  14. Lactate-activated macrophages induced aerobic glycolysis and epithelial-mesenchymal transition in breast cancer by regulation of CCL5-CCR5 axis: a positive metabolic feedback loop.
    Authors: Lin Et al.
    Oncotarget  2017;8:110426
  15. Specification of murine ground state pluripotent stem cells to regional neuronal populations.
    Authors: Alsanie
    Sci Rep  2017;7(1):16001
  16. Neuroprotective astrocyte-derived Ins/IGF-1 stimulates endocytic processing and extracellular release of neuron-bound Aβ oligomers.
    Authors: Pitt Et al.
    Mol Biol Cell  2017;28:2623
  17. Soluble Gamma-secretase Modulators Attenuate Alzheimer's β-amyloid Pathology and Induce Conformational Changes in Presenilin 1.
    Authors: Raven Et al.
    EBioMedicine  2017;24:93
  18. A modular platform for differentiation of human PSCs into all major ectodermal lineages.
    Authors: Tchieu Et al.
    Cell Stem Cell  2017;21:399
  19. Translocation of the papillomavirus L2/vDNA complex across the limiting membrane requires the onset of mitosis.
    Authors: Calton Et al.
    PLoS Pathog  2017;13:e1006200
  20. Efficient Generation of Hypothalamic Neurons from Human Pluripotent Stem Cells.
    Authors: Wang Et al.
    Curr Protoc Hum Genet  2016;90:21.5.1
  21. Canonical Notch signaling plays an instructive role in auditory supporting cell development.
    Authors: Campbell Et al.
    Zoological Lett  2016;6:19484
  22. Generation of serotonin neurons from human pluripotent stem cells.
    Authors: Lu Et al.
    Nat Biotechnol  2016;34:89
  23. Capturing the biology of disease severity in a PSC-based model of familial dysautonomia.
    Authors: Zeltner Et al.
    Nat Med  2016;22:1421
  24. Notch3 drives development and progression of cholangiocarcinoma
    Authors: Guest Et al.
    Proc Natl Acad Sci U S A.  2016;113:12250
  25. Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors.
    Authors: Edri Et al.
    Nat Commun  2015;6:6500
  26. Generation of clinical-grade human induced pluripotent stem cells in Xeno-free conditions.
    Authors: Wang Et al.
    Stem Cell Res Ther  2015;6:223
  27. On the origin of vertebrate somites.
    Authors: Onai Et al.
    Blood  2015;1:33
  28. A small molecule targeting ALK1 prevents Notch cooperativity and inhibits functional angiogenesis.
    Authors: Kerr Et al.
    Angiogenesis  2015;18:209
  29. Neurogenin 3 Expressing Cells in the Human Exocrine Pancreas Have the Capacity for Endocrine Cell Fate.
    Authors: Gomez Et al.
    Sci Rep  2015;10:e0133862
  30. Pleiotropic effects of Sox2 during the development of the zebrafish epithalamus.
    Authors: Pavlou Et al.
    PLoS One  2014;9:e87546
  31. Astrocyte-derived endothelin-1 inhibits remyelination through notch activation.
    Authors: Hammond Et al.
    Neuron  2014;81:588
  32. Canonical Notch signalling is inactive in urothelial carcinoma.
    Authors: Greife Et al.
    BMC Cancer  2014;14:628
  33. Mir-23a and mir-125b regulate neural stem/progenitor cell proliferation by targeting Musashi1.
    Authors: Gioia Et al.
    Anticancer Res  2014;11:1105
  34. Notch signaling regulates nucleocytoplasmic Olig2 translocation in reactive astrocytes differentiation after ischemic stroke.
    Authors: Marumo Et al.
    Neurosci Res  2013;75:204
  35. Generating regionalized neuronal cells from pluripotency, a step-by-step protocol.
    Authors: Kirkeby Et al.
    Front Cell Neurosci  2013;6:64
  36. Common developmental pathways link tooth shape to regeneration.
    Authors: Fraser Et al.
    Dev Biol  2013;377:399
  37. The role of Notch and γ-secretase inhibition in an ovarian cancer model.
    Authors: Shah Et al.
    J Neurosci  2013;33:801
  38. Presenilin1 regulates histamine neuron development and behavior in zebrafish, danio rerio.
    Authors: Sundvik Et al.
    J Neurosci  2013;33:1589
  39. Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia.
    Authors: Díaz Et al.
    J Cell Biol  2013;201:279
  40. Inflammation-mediated notch signaling skews fanconi anemia hematopoietic stem cell differentiation.
    Authors: Du Et al.
    J Immunol  2013;191:2806
  41. Shaping organs by a wingless-int/Notch/nonmuscle myosin module which orients feather bud elongation.
    Authors: Li Et al.
    Proc Natl Acad Sci U S A  2013;110:E1452
  42. Opposing effects of bacitracin on human papillomavirus type 16 infection: enhancement of binding and entry and inhibition of endosomal penetration.
    Authors: Campos Et al.
    J Virol  2012;86:4169
  43. Notch signaling inhibits axon regeneration.
    Authors: Bejjani and Hammarlund
    PLoS One  2012;73:268
  44. Fibulin-3 promotes glioma growth and resistance through a novel paracrine regulation of Notch signaling.
    Authors: Hu Et al.
    Cancer Res  2012;72:3873
  45. Jagged1 and Notch1 help edit M cell patterning in Peyer's patch follicle epithelium.
    Authors: Hsieh and Lo
    Dev Comp Immunol  2012;37:306
  46. Macrophage-derived Wnt opposes Notch signaling to specify hepatic progenitor cell fate in chronic liver disease.
    Authors: Boulter Et al.
    Nat Med  2012;18:572
  47. Notch and TGFβ form a reciprocal positive regulatory loop that suppresses murine prostate basal stem/progenitor cell activity.
    Authors: Valdez Et al.
    RNA Biol  2012;11:676
  48. Notch promotes vascular maturation by inducing integrin-mediated smooth muscle cell adhesion to the endothelial basement membrane.
    Authors: Scheppke Et al.
    Cell Stem Cell  2012;119:2149
  49. Characterization of full-length and proteolytic cleavage fragments of desmoglein-2 in native human colon and colonic epithelial cell lines.
    Authors: Kolegraff Et al.
    Cell Adh Migr  2011;5:306
  50. Mindbomb 1, an E3 ubiquitin ligase, forms a complex with RYK to activate Wnt/β-catenin signaling.
    Authors: Berndt Et al.
    J Cell Biol  2011;194:737
  51. Group II metabotropic glutamate receptor stimulation triggers production and release of Alzheimer's amyloid(β)42 from isolated intact nerve terminals.
    Authors: Kim Et al.
    Neuron  2010;30:3870

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Reviews for DAPT

Average Rating: 4.8 (Based on 6 Reviews)

5 Star
83.33%
4 Star
16.67%
3 Star
0%
2 Star
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For hPSC culture
By Anonymous on 06/30/2020
Application: Species: Human

For hPSC culture, final concentration 10 μM DAPT.


works well
By Anonymous on 03/26/2020
Application: Species: Rat

gamma -secretase (100 nM BMS 299897 and 250 nM DAPT)


The hiPSCs were maintained with Essential 8 Flex Medium with DAPT
By Anonymous on 02/08/2020
Application: Species: Human

2.5 μM

PMID: 29939162

DAPT succesful for dopaminergic neuron differentiation
By Anonymous on 01/23/2020
Application: Species: Human

Used successfully for the differentiation of iPSCs into dopaminergic neurons.


used in human cell line cultures
By Anonymous on 08/20/2019
Application: Species: Human

In cell line culture of human cells


Works great.
By Anonymous on 01/04/2018
Application: Species: Human

I used this product for differentiation of iPSCs into dopaminergic neurons according to the protocol published in Kriks et al, 2011.I used the concentration 10uM and it works really well.


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