Pygopus-2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20019

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Pygopus-2 Primary Antibodies »

Product Specifications

Immunogen

Full length mouse Pygopus-2 Recombinant protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Pygopus-2 Antibody - BSA Free

Immunohistochemistry-Paraffin: Pygopus-2 Antibody [NBP2-20019]

Immunohistochemistry-Paraffin: Pygopus-2 Antibody [NBP2-20019]

Immunohistochemistry-Paraffin: Pygopus-2 Antibody [NBP2-20019] - Pygopus-2 antibody detects Pygopus-2 protein at nucleus in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. Pygopus-2 antibody diluted at 1:500.
Western Blot: Pygopus-2 Antibody [NBP2-20019]

Western Blot: Pygopus-2 Antibody [NBP2-20019]

Western Blot: Pygopus-2 Antibody [NBP2-20019] - Pygopus-2 was immunoprecipitated from 5*107 cells of SW480 whole cell lysate using 1 ug of Pygopus-2 antibody. The precipitated Pygopus-2 was detected by Pygopus-2 Antibody diluted at 1:15000. A: IgG. B: Pygopus-2 antibody
Immunohistochemistry-Paraffin: Pygopus-2 Antibody [NBP2-20019]

Immunohistochemistry-Paraffin: Pygopus-2 Antibody [NBP2-20019]

Immunohistochemistry-Paraffin: Pygopus-2 Antibody [NBP2-20019] - Pygopus-2 antibody detects Pygopus-2 protein at nucleus in mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. Pygopus-2 antibody diluted at 1:500.
Immunoprecipitation: Pygopus-2 Antibody [NBP2-20019]

Immunoprecipitation: Pygopus-2 Antibody [NBP2-20019]

Immunoprecipitation: Pygopus 2 Antibody [NBP2-20019] - Pygopus 2 was immunoprecipitated from 5*10^7 cells of SW480 whole cell lysate using 1 ug of anti-pygopus 2 antibody. The precipitated pygopus 2 was detected by NBP2-20019 diluted at 1:15000. A: IP-IgG B: IP-pygopus 2 antibody.
Pygopus-2 Antibody - BSA Free

Western Blot: Pygopus-2 Antibody [NBP2-20019] -

Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Pygopus 2 antibody (NBP2-20019) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Pygopus-2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Pygopus-2 Antibody [NBP2-20019] -

Pygopus 2 antibody detects Pygopus 2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Pygopus 2 protein stained by Pygopus 2 antibody (NBP2-20019) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for Pygopus-2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Application Notes
ChIP assay- Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Pygopus-2

ATPase which is responsible for recognizing, binding, unfolding and translocation of pupylated proteins into the bacterial 20S proteasome core particle. May be essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase may function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis. Is required but not sufficient to confer resistance against the lethal effects of reactive nitrogen intermediates (RNI), antimicrobial molecules produced by activated macrophages and other cell types.

Alternate Names

PYGO2, Pygopus2

Gene Symbol

PYGO2

Additional Pygopus-2 Products

Product Documents for Pygopus-2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Pygopus-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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