QKI/Quaking Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87178

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all QKI/Quaking Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RAEIKLKRAVEEVKKLLVPAAEGEDSLKKMQLMELAILNGTYRDANIKSPALAFSLAATAQAAPRIITGPAPVLPPAALRTPTPAGPTIMPLIRQIQTAVMPNGTPHPTAAIVPPGPEAGLIYTPYEYPYTLAPATSILEYPIEPSGVL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for QKI/Quaking Antibody - BSA Free

Western Blot: QKI/Quaking Antibody [NBP1-87178]

Western Blot: QKI/Quaking Antibody [NBP1-87178]

Western Blot: QKI/Quaking Antibody [NBP1-87178] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody [NBP1-87178]

Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody [NBP1-87178]

Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody [NBP1-87178] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178]

Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178]

Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178] - Staining in human cerebral cortex and pancreas tissues. Corresponding QKI RNA-seq data are presented for the same tissues.
Western Blot: QKI/Quaking Antibody [NBP1-87178]

Western Blot: QKI/Quaking Antibody [NBP1-87178]

Western Blot: QKI/Quaking Antibody [NBP1-87178] - Analysis in human cerebral cortex tissue.
Immunohistochemistry: QKI/Quaking Antibody [NBP1-87178]

Immunohistochemistry: QKI/Quaking Antibody [NBP1-87178]

Immunohistochemistry: QKI/Quaking Antibody [NBP1-87178] - Staining of mouse brain shows strong positivity in a subset of glial cells in the cerebral cortex.
Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178]

Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178]

Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178] - Staining of human cerebellum shows moderate to strong nuclear positivity in glial cells.
Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178]

Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178]

Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in glial cells.
Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178]

Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178]

Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
QKI/Quaking Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: QKI/Quaking Antibody - BSA Free [NBP1-87178]

Chromatin Immunoprecipitation-exo-Seq: QKI/Quaking Antibody - BSA Free [NBP1-87178]

ChIP-Exo-Seq composite graph for Anti-QKI (NBP1-87178) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for QKI/Quaking Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: QKI/Quaking

QKI belongs to a family of RNA-binding proteins that have an HNRNPK (MIM 600712) homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1; MIM 602489) and SF1 (MIM 601516) (Chen and Richard, 1998).[supplied by OMIM]

Alternate Names

DKFZp586I0923, HKQ, homolog of mouse quaking QKI (KH domain RNA binding protein), Hqk, HqkI, protein quaking, QK, QK1, QK3, quaking homolog, KH domain RNA binding (mouse), RNA binding protein HQK

Gene Symbol

QKI

Additional QKI/Quaking Products

Product Documents for QKI/Quaking Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for QKI/Quaking Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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