Rab25 Antibody (3F12)

Novus Biologicals | Catalog # NBP1-51599

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Western Blot, IF/IHC, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3F12
View all Rab25 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of Rab25 expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rab25 Antibody (3F12)

Western Blot: Rab25 Antibody (3F12)BSA Free [NBP1-51599]

Western Blot: Rab25 Antibody (3F12)BSA Free [NBP1-51599]

Western Blot: Rab25 Antibody (3F12) [NBP1-51599] - Analysis using Rab25 mouse mAb against MCF-7 (1), T47D (2) and GC7901 (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: Rab25 Antibody (3F12) - BSA Free [NBP1-51599]

Immunocytochemistry/ Immunofluorescence: Rab25 Antibody (3F12) - BSA Free [NBP1-51599]

Immunocytochemistry/Immunofluorescence: Rab25 Antibody (3F12) [NBP1-51599] - Analysis of A549 cells using RAB25 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Rab25 Antibody (3F12) - BSA Free [NBP1-51599]

Immunohistochemistry-Paraffin: Rab25 Antibody (3F12) - BSA Free [NBP1-51599]

Immunohistochemistry-Paraffin: Rab25 Antibody (3F12) [NBP1-51599] - Analysis of esophagus tissues (left) and human lung cancer (right) using Rab25 mouse mAb with DAB staining.
Flow Cytometry: Rab25 Antibody (3F12) - BSA Free [NBP1-51599]

Flow Cytometry: Rab25 Antibody (3F12) - BSA Free [NBP1-51599]

Flow Cytometry: Rab25 Antibody (3F12) [NBP1-51599] - Analysis of NIH/3T3 cells using Rab25 mouse mAb (green) and negative control (purple).

Applications for Rab25 Antibody (3F12)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
Knockdown validation (PMID: 31564077).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rab25

Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa

Long Name

RAs Genes from Brain Protein 25

Alternate Names

CATX8, RAB11C

Gene Symbol

RAB25

Additional Rab25 Products

Product Documents for Rab25 Antibody (3F12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Rab25 Antibody (3F12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy
Cancer Biomarkers

Citations for Rab25 Antibody (3F12)

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Protocols

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FAQs

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