RAB6A Antibody (HL1047) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13701

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat, Drosophila, Zebrafish

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1047 expressed in HEK293

Format

Azide and BSA Free
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Product Specifications

Immunogen

Full length human RAB6A recombinant protein.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for RAB6A Antibody (HL1047) - Azide and BSA Free

Western Blot: RAB6A Antibody (HL1047) [NBP3-13701]

Western Blot: RAB6A Antibody (HL1047) [NBP3-13701]

Western Blot: RAB6A Antibody (HL1047) [NBP3-13701] - Wild-type (WT) and RAB6A knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody [HL1047] (NBP3-13701) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: RAB6A Antibody (HL1047) [NBP3-13701]

Immunohistochemistry-Paraffin: RAB6A Antibody (HL1047) [NBP3-13701]

Immunohistochemistry-Paraffin: RAB6A Antibody (HL1047) [NBP3-13701] - RAB6A antibody [HL1047] detects RAB6A protein at Golgi apparatus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. RAB6A stained by RAB6A antibody [HL1047] (NBP3-13701) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: RAB6A Antibody (HL1047) [NBP3-13701]

Western Blot: RAB6A Antibody (HL1047) [NBP3-13701]

Western Blot: RAB6A Antibody (HL1047) [NBP3-13701] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody [HL1047] (NBP3-13701) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: RAB6A Antibody (HL1047) [NBP3-13701]

Western Blot: RAB6A Antibody (HL1047) [NBP3-13701]

Western Blot: RAB6A Antibody (HL1047) [NBP3-13701] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody [HL1047] (NBP3-13701) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
RAB6A Antibody (HL1047)

Western Blot Shows Human RAB6A Specificity Using Knockout Cell Line.

Western blot shows lysates of HAP1 cell line and RAB6A knockout HAP1 cell line (KO). Nitrocellulose membrane was probed with RAB6A Antibody (HL1047) (Catalog # NBP3-13701) followed by HRP-conjugated secondary antibody. A specific band was detected for RAB6A at approximately 24 kDa (as indicated) in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line. Primary antibody dilution used: 1/200. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.
RAB6A Antibody (HL1047) - Azide and BSA Free

Western Blot: RAB6A Antibody (HL1047) [NBP3-13701] -

Whole zebrafish extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody [HL1047] (NBP3-13701) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
RAB6A Antibody (HL1047) - Azide and BSA Free

Western Blot: RAB6A Antibody (HL1047) [NBP3-13701] -

Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody [HL1047] (NBP3-13701) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for RAB6A Antibody (HL1047) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Knockout Validated

Validated for Knockout from YCharOS Inc. (ycharos.com)

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RAB6A

Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Has a low GTPase activity

Alternate Names

GTP-binding protein RAB6A', GTP-binding protein RAB6B, Oncogene RAB6, Rab GTPase, Rab-6, RAB6, member RAS oncogene family, RAB6A', Rab6A variant 3, RAB6A, member RAS oncogene family, RAB6B, RAB6rab-6, ras-related protein Rab-6A

Gene Symbol

RAB6A

Additional RAB6A Products

Product Documents for RAB6A Antibody (HL1047) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RAB6A Antibody (HL1047) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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