RAB6A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33110

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (98%), Chicken (97%), Porcine (97%), Rhesus Macaque (98%), Xenopus (96%), Zebrafish (98%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RAB6A Primary Antibodies »

Product Specifications

Immunogen

Full length human RAB6A Recombinant protein.

Reactivity Notes

Xenopus laevis (96%).

Localization

Golgi apparatus membrane, Lipid-anchor

Marker

Golgi Apparatus Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RAB6A Antibody - BSA Free

Western Blot: RAB6A Antibody [NBP1-33110]

Western Blot: RAB6A Antibody [NBP1-33110]

Western Blot: RAB6A Antibody [NBP1-33110] - A. 50 ug mouse brain lysate/extract 12 % SDS-PAGE RAB6A antibody dilution: :1000
Immunocytochemistry/ Immunofluorescence: RAB6A Antibody [NBP1-33110]

Immunocytochemistry/ Immunofluorescence: RAB6A Antibody [NBP1-33110]

Immunocytochemistry/Immunofluorescence: RAB6A Antibody [NBP1-33110] - Analysis of Human RPE1 cells using RAB6A antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: RAB6A Antibody [NBP1-33110]

Immunohistochemistry-Paraffin: RAB6A Antibody [NBP1-33110]

Immunohistochemistry-Paraffin: RAB6A Antibody [NBP1-33110] - MDA-MB-468 xenograft, using RAB6A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: RAB6A Antibody [NBP1-33110]

Western Blot: RAB6A Antibody [NBP1-33110]

Western Blot: RAB6A Antibody [NBP1-33110] - A. 50 ug rat brain lysate/extract 12 % SDS-PAGE RAB6A antibody dilution: 1:1000
Knockout Validated: RAB6A Antibody [NBP1-33110]

Western Blot: RAB6A Antibody [NBP1-33110]

Western Blot: RAB6A Antibody [NBP1-33110] - Wild-type (WT) and RAB6A knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
RAB6A Antibody

Western Blot Shows Human RAB6A Specificity Using Knockout Cell Line.

Western blot shows lysates of HAP1 cell line and RAB6A knockout HAP1 cell line (KO). Nitrocellulose membrane was probed with RAB6A Antibody (Catalog # NBP1-33110) followed by HRP-conjugated secondary antibody. A specific band was detected for RAB6A at approximately 24 kDa (as indicated) in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line. Primary antibody dilution used: 1/1000. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.
RAB6A Antibody

RAB6A Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

HAP1 WT and RAB6A KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with RAB6A Antibody (Catalog # NBP1-33110) and with an Alexa-fluor 555 coupled secondary antibody including DAPI. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Primary antibody dilution used: 1/800. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
RAB6A Antibody

Western Blot: RAB6A Antibody [NBP1-33110] -

Western Blot: RAB6A Antibody [NBP1-33110] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody (NBP1-33110) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for RAB6A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 5 using NBP1-33110 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RAB6A

Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Has a low GTPase activity

Alternate Names

GTP-binding protein RAB6A', GTP-binding protein RAB6B, Oncogene RAB6, Rab GTPase, Rab-6, RAB6, member RAS oncogene family, RAB6A', Rab6A variant 3, RAB6A, member RAS oncogene family, RAB6B, RAB6rab-6, ras-related protein Rab-6A

Gene Symbol

RAB6A

Additional RAB6A Products

Product Documents for RAB6A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RAB6A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RAB6A Antibody - BSA Free

Customer Reviews for RAB6A Antibody - BSA Free (1)

5 out of 5
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Customer Images

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  • RAB6A Antibody
    Name: Boyan Zhang
    Application: Immunocytochemistry
    Sample Tested: RPE1 cells
    Species: Human
    Verified Customer | Posted 08/23/2022
    Very good for detecting endogenous Rab6 protein in the Golgi
    RAB6A Antibody - BSA Free NBP1-33110

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