Rab7a Antibody (5A10A4)

Rab7a Antibody (4X3P6) [NBP3-15444] -

Confocal imaging of NIH/3T3 cells using RAB7 Rabbit mAb (dilution 1:2000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Rab7a Antibody (4X3P6) [NBP3-15444] -

RAB7 from 300 µg extracts of HeLa cells was performed using 0.5 µg of RAB7 Rabbit mAb. Rabbit Control IgG (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1x Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using RAB7 Rabbit mAb at a dilution of 1:5000.

Rab7a Antibody (4X3P6) [NBP3-15444] -

Analysis of paraffin-embedded Mouse testis tissue using RAB7 Rabbit mAb at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.