Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (98%), Canine (98%), Fish (98%), Primate (98%), Rabbit (96%), Rat (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

A portion of amino acids 90-140 of human Rab7 protein was used as the immunogen for this antibody.

Marker

Late Endosome Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Rab7a Antibody - BSA Free (NBP2-24591) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Rab7a Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Rab7a Antibody - BSA Free

Western Blot: Rab7a AntibodyBSA Free [NBP2-24591]

Western Blot: Rab7a AntibodyBSA Free [NBP2-24591]

Western Blot: Rab7a Antibody [NBP2-24591] - Analysis of human Rab7 in HeLa lysate in the 1) absence and 2) presence of immunizing peptide 3) mouse NIH 3T3 and 4) RAW using Rab7 at 0.25 ug/ml.
Immunocytochemistry/ Immunofluorescence: Rab7a Antibody - BSA Free [NBP2-24591]

Immunocytochemistry/ Immunofluorescence: Rab7a Antibody - BSA Free [NBP2-24591]

Immunocytochemistry/Immunofluorescence: Rab7a Antibody [NBP2-24591] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Rab7A at 2ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Rab7a Antibody - BSA Free [NBP2-24591]

Immunohistochemistry-Paraffin: Rab7a Antibody - BSA Free [NBP2-24591]

Immunohistochemistry-Paraffin: Rab7a Antibody [NBP2-24591] - Analysis of human skeletal muscle using RAB7A antibody at 10 ug/ml.
Immunocytochemistry/ Immunofluorescence: Rab7a Antibody - BSA Free [NBP2-24591]

Immunocytochemistry/ Immunofluorescence: Rab7a Antibody - BSA Free [NBP2-24591]

Immunocytochemistry/Immunofluorescence: Rab7a Antibody [NBP2-24591] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Rab7A at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Applications for Rab7a Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-5 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

0.5-1 ug/ml

Reviewed Applications

Read 1 review rated 3 using NBP2-24591 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Rab7a

RAB7 is a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies.

Long Name

RAs Genes from Brain Protein 7a

Alternate Names

PRO2706, RAB7

Gene Symbol

RAB7A

UniProt

P51149 (Human)

Additional Rab7a Products

Product Documents for Rab7a Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Rab7a Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy
Diabetic Peripheral Neuropathy

Citations for Rab7a Antibody - BSA Free

Customer Reviews for Rab7a Antibody - BSA Free (1)

3 out of 5
1 Customer Rating
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Customer Images

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  • Rab7a Antibody
    Name: Teddy Chan
    Application: Western Blot
    Sample Tested: Human microglia cell lysate
    Species: Human
    Verified Customer | Posted 02/08/2019
    Do not boil samples, leave in loading dye 1hr at RT. Antibody picks up multiple non-specific bands
    Rab7a Antibody - BSA Free NBP2-24591

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Protocols

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