V5 Epitope Tag Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8926
Detection of  V5 Epitope-tagged proteins by Western Blot.
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V5 Epitope Tag Antibody Summary

Specificity
Detects N-terminal and C-terminal V5 epitope-tagged proteins in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1036H
Purification
Protein A or G purified from cell culture supernatant
Immunogen
V5 epitope tag peptide
Accession # Y138513
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
Immunoprecipitation
1-4 µg/100 µg cell lysate
HEK293 human embryonic kidney cell line transfected with N-terminal V5-tagged FDNC4 and C-terminal V5-tagged EAA1, see our available Western blot detection antibodies.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of  V5 Epitope-tagged proteins by Western Blot. View Larger

Detection of V5 Epitope-tagged proteins by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal V5-tagged CBL, N-terminal V5-tagged FSNC4, C-terminal V5-tagged LAMP2B, and C-terminal V5-tagged GALNT2. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-V5 Epitope Tag Monoclonal Antibody (Catalog # MAB8926) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for V5 Epitope-tagged at approximately 110 kDa, 34 kDa, 75 kDa, and 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of V5 Epitope Tag in HEK293 Human Cell Line Transfected with V5-tagged Proteins by Flow Cytometry. View Larger

Detection of V5 Epitope Tag in HEK293 Human Cell Line Transfected with V5-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with V5-tagged proteins was stained with Rabbit Anti-V5 Epitope Tag Monoclonal Antibody (Catalog # MAB8926, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).

Simple Western Detection of  V5 Epitope Tag by Simple WesternTM. View Larger

Detection of V5 Epitope Tag by Simple WesternTM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal V5-tagged CBL and C-terminal V5-tagged GALNT2, loaded at 0.2 mg/mL. Specific bands were detected for V5 Epitope-tagged proteins at approximately 119 kDa and 65 kDa (as indicated) using 1 µg/mL of Rabbit Anti-V5 Epitope Tag Monoclonal Antibody (Catalog # MAB8926). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: V5 Epitope Tag

Entrez Gene IDs
3160800 (Viral)
Alternate Names
GKPIPNPLLGLDST Epitope Tag; Parainfluenza virus 5 V/P; Paramyxovirus SV5; V5 Epitope Tag

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

ExactaChIP Chromatin Immunoprecipitation Kits

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

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