Rad50 Antibody (13B3) - Azide and BSA Free

Novus Biologicals | Catalog # NB100-147

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In vitro assay, Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 13B3

Format

Azide and BSA Free
View all Rad50 Primary Antibodies »

Product Specifications

Immunogen

Amino acids 1-425 of Rad50 expressed in E. coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Nuclear

Specificity

This is specific for Rad50.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

153 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rad50 Antibody (13B3) - Azide and BSA Free

Immunohistochemistry-Paraffin: Rad50 Antibody (13B3) [NB100-147]

Immunohistochemistry-Paraffin: Rad50 Antibody (13B3) [NB100-147]

Immunohistochemistry-Paraffin: Rad50 Antibody (13B3) [NB100-147] - Human lung cancer. Rad50 stained by Rad50 antibody [13B3] diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Rad50 Antibody (13B3) [NB100-147]

Western Blot: Rad50 Antibody (13B3) [NB100-147]

Western Blot: Rad50 Antibody (13B3) [NB100-147] - Analysis of RAD50 in 293T cell lysate using anti-RAD50 antibody.Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Rad50 Antibody (13B3) [NB100-147]

Immunohistochemistry-Paraffin: Rad50 Antibody (13B3) [NB100-147]

Immunohistochemistry-Paraffin: Rad50 Antibody (13B3) [NB100-147] - CAL 27 xenograft. Rad50 antibody [13B3] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Rad50 Antibody (13B3) [NB100-147]

Immunohistochemistry-Paraffin: Rad50 Antibody (13B3) [NB100-147]

Immunohistochemistry-Paraffin: Rad50 Antibody (13B3) [NB100-147] - PC-3 xenograft. Rad50 antibody [13B3] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Rad50 Antibody (13B3) [NB100-147]

Immunohistochemistry-Paraffin: Rad50 Antibody (13B3) [NB100-147]

Immunohistochemistry-Paraffin: Rad50 Antibody (13B3) [NB100-147] - Human lung cancer. Rad50 stained by Rad50 antibody [13B3] diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Knockdown Validated: Rad50 Antibody (13B3) [NB100-147]

Western Blot: Rad50 Antibody (13B3) [NB100-147]

Western Blot: Rad50 Antibody (13B3) [NB100-147] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3].
Rad50 Antibody (13B3)

Western Blot: Rad50 Antibody (13B3) [NB100-147] -

Western Blot: Rad50 Antibody (13B3) [NB100-147] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (NB100-147) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Rad50 Antibody (13B3) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Rad50 Antibody (13B3) [NB100-147] -

Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rad50 protein stained by Rad50 antibody [13B3] (NB100-147) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 ?m.
Rad50 Antibody (13B3) - Azide and BSA Free

Western Blot: Rad50 Antibody (13B3) [NB100-147] -

HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (NB100-147) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Applications for Rad50 Antibody (13B3) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100 - 1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
IHC usage reported in scientific literature (PMID: 16431910). in vitro Assay dependent, IP Assay dependent. Rad50 antibody validated for WB from a verified customer review.

Reviewed Applications

Read 1 review rated 4 using NB100-147 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rad50

Human Rad50, a 153kDa protein involved in DNA double strand break repair, is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex involved in the double strand break repair process. Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells

Alternate Names

DNA repair protein RAD50, EC 3.6, EC 3.6.1.15, EC 3.6.3, EC 3.6.3.27, hRAD50, NBSLD, RAD50 (S. cerevisiae) homolog, RAD50 homolog (S. cerevisiae), RAD502, RAD50-2

Entrez Gene IDs

10111 (Human)

Gene Symbol

RAD50

Additional Rad50 Products

Product Documents for Rad50 Antibody (13B3) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Rad50 Antibody (13B3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Rad50 Antibody (13B3) - Azide and BSA Free

Customer Reviews for Rad50 Antibody (13B3) - Azide and BSA Free (1)

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Customer Images

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  • Name: Anatoli Grechov
    Application: Western Blot
    Sample Tested: 293T lysate
    Species: Human
    Verified Customer | Posted 12/17/2014
    RAD50 WB in 293T cells
    Rad50 Antibody (13B3) - Azide and BSA Free NB100-147

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