Rad51 Antibody (14B4) - Azide and BSA Free

Novus Biologicals | Catalog # NB100-148

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Cited:

Human, Mouse, Nematode - Caenorhabditis elegans

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), In vitro assay, Proximity Ligation Assay, Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Chemotaxis, In vitro assay, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 14B4

Format

Azide and BSA Free
View all Rad51 Primary Antibodies »

Product Specifications

Immunogen

Full length (amino acids 1-338) Rad51 expressed in E. coli.

Reactivity Notes

C. elegans reactivity reported in scientific literature (PMID: 23942865). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse Rad51 Antibody (14B4) - Azide and BSA Free (NB100-148) is a monoclonal antibody validated for use in IHC, WB, ICC/IF, IP and ChIP. Anti-Rad51 Antibody: Cited in 68 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Rad51 Antibody (14B4) - Azide and BSA Free

Western Blot: Rad51 Antibody (14B4) [NB100-148]

Western Blot: Rad51 Antibody (14B4) [NB100-148]

Rad51-Antibody-14B4-Western-Blot-NB100-148-img0020.jpg
Immunocytochemistry/ Immunofluorescence: Rad51 Antibody (14B4) [NB100-148]

Immunocytochemistry/ Immunofluorescence: Rad51 Antibody (14B4) [NB100-148]

Immunocytochemistry/Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] - HCC1937 (human immortalized breast cancer) cells treated with PARPi for 96 h. Nuclei are stained in blue (DAPI) and RAD51 in red. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Rad51 Antibody (14B4) [NB100-148]

Immunohistochemistry-Paraffin: Rad51 Antibody (14B4) [NB100-148]

Immunohistochemistry-Paraffin: Rad51 Antibody (14B4) [NB100-148] - BT483 xenograft. RAD51 antibody 14B4 dilution at 1:200. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15 min.
Western Blot: Rad51 Antibody (14B4) [NB100-148]

Western Blot: Rad51 Antibody (14B4) [NB100-148]

Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blot: Rad51 Antibody (14B4) [NB100-148]

Western Blot: Rad51 Antibody (14B4) [NB100-148]

Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunocytochemistry/ Immunofluorescence: Rad51 Antibody (14B4) [NB100-148]

Immunocytochemistry/ Immunofluorescence: Rad51 Antibody (14B4) [NB100-148]

Immunocytochemistry/Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] - Staining of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody. Cells were pre-extracted with CSK buffer before fixation with 4% PFA. RAD51 14B4 was used at 1:1000 dultion. DAPI was used to counterstain the nucleus. Scale bar, 10 mciro-meter.
Immunoprecipitation: Rad51 Antibody (14B4) [NB100-148]

Immunoprecipitation: Rad51 Antibody (14B4) [NB100-148]

Immunoprecipitation: Rad51 Antibody (14B4) [NB100-148] - HeLa whole cell extract prepared with lysis 180 buffer (40 mM Tris-HCl pH8.0, 180 mM NaCl, 1 mMEDTA, 0.5% NP-40). Rabbit anti-RAD51 antibody was used for subsequent WB detection of immunoprecipated RAD51.
Knockdown Validated: Rad51 Antibody (14B4) [NB100-148]

Western Blot: Rad51 Antibody (14B4) [NB100-148]

Western Blot: Rad51 Antibody (14B4) [NB100-148] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4].
Rad51 Antibody (14B4)

Western Blot: Rad51 Antibody (14B4) [NB100-148] -

Western Blot: Rad51 Antibody (14B4) [NB100-148] - YFP-PALB2 & YFP-PALB2 146AAAA binds RAD51 equally.Lysates from HEK293T cells expressing the indicated constructs were subjected to GFP-Trap pulldown & immunoblotting against YFP or RAD51. YFP alone was used as a negative control. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31017574), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Rad51 Antibody (14B4)

Immunocytochemistry/ Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] -

Immunocytochemistry/ Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] - Bractoppin Selectively Interrupts BRCA1-Dependent Steps in DNA Repair by Homologous Recombination(A) Confocal images depicting at high magnification the recruitment of the RPA32 protein into nuclear foci after the indicated treatments. Experiments were carried out as described as in Figure 5A. Staining in the upper row is for RPA32 (green), middle row, for mCherry-BRCA1 tBRCT (red); lower row, merged red & green staining, with DNA staining (DAPI) in blue. Scale bar represents 10 μm.(B) Recruitment of RAD51 protein into nuclear foci, measured & depicted as described in (A). Scale bar represents 10 μm.(C) Percentage of cells positive for radiation-induced nuclear RPA32 foci (mean ± SD; n = 5,300, 0 Gy; 3,200, 16 Gy; 3,600, BRCA1 tBRCT; 3,400, Bractoppin; 3,500, CCBT2047) enumerated by high-content imaging (see the STAR Methods). Statistical significance was performed using an unpaired two-tailed t test. ***p ≤ 0.001. Similar results were observed in three independent repeats.(D) Percentage of cells containing nuclear RAD51 foci enumerated & depicted as described in (B). ***p ≤ 0.001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29606576), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Rad51 Antibody (14B4)

Western Blot: Rad51 Antibody (14B4) [NB100-148] -

Western Blot: Rad51 Antibody (14B4) [NB100-148] - MiCas9’s beneficial effects are Brex27 & RAD51 dependent.a Indel rates by spCas9, miCas9, & miCas9-mutant at the on-target site for sg-AAVS1 & the off-target site 1 for sg-EMX1. b Indel rates estimated by T7E1 assay by spCas9 & miCas9 with or without the use of RAD51 inhibitor, B02. c Co-IP results by using anti-flag (left) or anti-RAD51 (right) antibodies followed western blot for RAD51 or Cas9. d ChIP assay results with anti-RAD51 antibody at the proximal region of the sg-AAVS1 target locus. Sp: spCas9, Mi: miCas9, Mi-mut: miCas9-mutant. #Reads: Average amplicon reads per sample. Three independent experiments were performed for each condition. Data are presented as mean ± standard error of means (SEM). Unpaired t-test (two tailed) was used to compare data using GraphPad Prism 8 software (GraphPad Software, Inc., San Diego, CA). Source data are available in the Source Data file. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33247137), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Rad51 Antibody (14B4) - Azide and BSA Free

Western Blot: Rad51 Antibody (14B4) [NB100-148] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (NB100-148) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Applications for Rad51 Antibody (14B4) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:1000

Immunohistochemistry

1:100 - 1:1000

Immunohistochemistry-Paraffin

1:100 - 1:1000

Western Blot

1:500 - 1:3000
Application Notes
ChIP usage reported in scientific literature (PMID: 24023853). Rad51 antibody validated for IHC-Fr, ICC/IF from verified customer reviews. Use in In vitro assay reported in scientific literature (PMID: 27815389). PLA-Assay dependent.

Reviewed Applications

Read 5 reviews rated 4.8 using NB100-148 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rad51

Rad51, a 37 kDa protein, is the human homolog of E. coli RecA protein and a member of the RAD52 epistasis group in S. cerevisiae. In recent studies it has been reported that BRCA1 interacts with Rad51 and disease-causing mutations have been found in the BRCA1 region necessary for BRCA1/Rad51 interaction, implying that this interaction is important for tumor suppression. BRCA2 and Rad51 have also been shown to interact by direct binding of the BRC repeats located within exon 11 of BRCA2. When these repeats are deleted, the interaction is lost and cells become hypersensitive to the DNA damage caused by methyl methanesulfonate (MMS).

Alternate Names

BRCC5, hRAD51, HsRad51, HsT16930, RAD51 (S. cerevisiae) homolog (E coli RecA homolog), RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae), RAD51 homolog A, RAD51ABRCA1/BRCA2-containing complex, subunit 5, RAD51L3, RecA, E. coli, homolog of, RECADNA repair protein RAD51 homolog 1, RecA-like protein, recombination protein A

Entrez Gene IDs

5888 (Human)

Gene Symbol

RAD51

UniProt

Q06609

Additional Rad51 Products

Product Documents for Rad51 Antibody (14B4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Rad51 Antibody (14B4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Rad51 Antibody (14B4) - Azide and BSA Free

Customer Reviews for Rad51 Antibody (14B4) - Azide and BSA Free (5)

4.8 out of 5
5 Customer Ratings
5 Stars
80%
4 Stars
20%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Rad51 Antibody (14B4) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 5 of 5 reviews Showing All
Filter By:
Clear Filters X
  • Rad51 Antibody (14B4)
    Name: Marina Rodriguez Candela
    Application: Immunocytochemistry
    Sample Tested: human immortalized breast cancer cells
    Species: Human
    Verified Customer | Posted 06/14/2021
    HCC1937 cells treated with PARPi for 96 h. Nuclei are stained in blue (DAPI) and RAD51 in red.
    Cells were fixed with 4% PFA. RAD51 Ig was diluted 1:200.
    Rad51 Antibody (14B4) - Azide and BSA Free NB100-148
  • Rad51 Antibody (14B4)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: testis cells
    Species: Mouse
    Verified Customer | Posted 10/09/2019
    We used NB100-148 antibody to detect Rad51 foci in germ cell, and colocalize with dmc1(red color).
    Rad51 Antibody (14B4) - Azide and BSA Free NB100-148
  • Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: See PMID 23942865
    Species: Other
    Verified Customer | Posted 12/12/2014
  • Name: Manuela Buonanno
    Application: Immunohistochemistry-Frozen
    Sample Tested: whole C. elegans nematode
    Species: Other
    Verified Customer | Posted 11/07/2013
  • Name: Western Forte
    Application: Western Blot
    Sample Tested: Hela whole cell lysate
    Species: Human
    Verified Customer | Posted 06/20/2012
    Rad51 Antibody (14B4) - Azide and BSA Free NB100-148

There are no reviews that match your criteria.

Showing  1 - 5 of 5 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies