Rat alpha 1-Microglobulin Antibody

Catalog # Availability Size / Price Qty
Detection of Rat  alpha 1-Microglobulin by Western Blot.
1 Image
Product Details
Supplemental Products

Rat alpha 1-Microglobulin Antibody Summary

Species Reactivity
Detects rat alpha 1-Microglobulin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human alpha 1-Microglobulin is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant rat alpha 1-Microglobulin
Accession # Q64240
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Rat a1-Microglobulin antibody by Western Blot. View Larger

Detection of Rat alpha 1-Microglobulin by Western Blot. Western blot shows rat plasma. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Rat a1-Microglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7720) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a1-Microglobulin at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha 1-Microglobulin

Rat alpha 1-Microglobulin (alpha1-m/A1M; also protein HC) is a secreted, 25-26 kDa glycoprotein member of the lipocalin family, calycin superfamily of molecules. It is expressed by hepatocytes, keratinocytes, and endodermal derivatives in the embryo. A1M appears to act as a heme scavenger, protecting cells and collagen against oxidative damage. It also acts as an immunosuppressant, inhibiting polyclonal lymphocyte activation and dampening granulocyte migration in response to chemokines. A1M circulates either as a monomer, or bound to IgA, albumin or prothrombin. Rat A1M is generated through cleavage of a precursor molecule termed AMBP. This AMBP should not be confused with AMBP-1, a 120-140 kDa adrenomedullin-binding protein that is also known as Complement Factor H. The AMBP precursor contains a 19 aa signal sequence, an N-terminal 183 aa A1M protein (aa 20-202), and a C-terminal serine protease inhibitor termed bikunin (aa 205-349). A1M possesses one lipocalin domain (aa 41-186). Although cleavage of AMBP in the Golgi apparatus typically generates a 25 kDa A1M and 28 kDa bikunin molecule, the 55-65 kDa AMBP precursor can also be released intact. In human, A1M will undergo extracellular processing, generating an isoform that is missing the C-terminal four amino acids. There are four potential isoform variants. One utilizes an alternative start site at Met181, a second contains an Asn substitution for aa 112-228, a third possesses an 11 aa substitution for aa 1-141, and a fourth shows a 17 aa substitution for aa 201-349. Over aa 20-202, rat A1M shares 76% and 86% aa sequence identity with human and mouse A1M, respectively.

Entrez Gene IDs
259 (Human); 11699 (Mouse); 25377 (Rat)
Alternate Names
A1M; alpha 1Microglobulin; alpha 1-Microglobulin; alpha-1-microglobulin/bikunin precursor; AMBP; bikunin; EDC1; HCP; HCPcomplex-forming glycoprotein heterogeneous in charge; HI30; IATIL; inter-alpha-trypsin inhibitor light chain; ITI; ITIL; ITILC; ITILgrowth-inhibiting protein 19; protein AMBP; protein HC; trypstatin; uristatin; uronic-acid-rich protein; UTI

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Rat alpha 1-Microglobulin Antibody

There are currently no reviews for this product. Be the first to review Rat alpha 1-Microglobulin Antibody and earn rewards!

Have you used Rat alpha 1-Microglobulin Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review