Rat CCL3/MIP-1 alpha Antibody

Catalog # Availability Size / Price Qty
MAB66252
MAB66252-SP
Detection of Rat CCL3/MIP‑1 alpha  by Western Blot.
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Product Details
Citations (1)
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Rat CCL3/MIP-1 alpha Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat CCL3/MIP‑1 alpha in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 769324
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant rat CCL3/MIP‑1 alpha
Ala24-Ala92
Accession # P50229
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Neutralization
Measured by its ability to neutralize CCL3/MIP‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.075-0.45 μg/mL in the presence of 10 ng/mL Recombinant Rat CCL3/MIP‑1 alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Rat CCL3/MIP-1a antibody by Western Blot. View Larger

Detection of Rat CCL3/MIP‑1 alpha by Western Blot. Western blot shows lysates of NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Rat CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB66252) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CCL3/MIP-1a at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neutralization Chemotaxis Induced by CCL3/MIP‑1 alpha  and Neutral-ization by Rat CCL3/ MIP‑1 alpha  Antibody. View Larger

Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutral-ization by Rat CCL3/ MIP‑1 alpha Antibody. Recombinant Rat CCL3/MIP-1a (Catalog # 6625-MA) induces chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Rat CCL3/MIP-1a (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB66252). The ND50 is typically 0.075-0.45 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL3/MIP-1 alpha

CCL3, also known as macrophage inflammatory protein 1 alpha (MIP-1 alpha ) and LD78, is a member of the beta or CC subfamily of chemokines and is closely related to CCL4/MIP-1 beta. Chemokines comprise a large family of small secreted proteins that are involved in immune and inflammatory responses. CCL3 expression can be induced in a variety of hematopoietic cells, fibroblasts, smooth muscle cells, and epithelial cells (1). Mature rat CCL3 shares 74%, 91%, and 88% amino acid sequence identity with human, mouse, and cotton rat CCL3, respectively (2). CCL3 is an approximately 8 kDa chemokine that forms complexes with sulfated proteoglycans (3, 4). In a reversible process, CCL3 associates into noncovalently-linked dimers which then form tetramers and high molecular weight polymers (5, 6). These complexes of CCL3 are protected from proteolytic digestion by insulin degrading enzyme (IDE) which can cleave the monomeric chemokine (6). CCL3 exerts its biological functions through interactions with CCR1, CCR3, and CCR5 (1). It is cleared from the extracellular space by internalization via the decoy chemokine receptor D6 (7). CCL3 promotes the chemoattraction, adhesion to activated vascular endothelium, and cellular activation of many hematopoietic cell types including activated T cells, NK cells, neutrophils, monocytes, immature dendritic cells, and eosinophils (1, 8-10). CCL3 is also known as stem cell inhibitor (SCI) and can inhibit the proliferation of hematopoietic progenitor cells (3). CCL3 bioactivity contributes to tumor metastasis and the inflammatory components of viral infection, rheumatoid arthritis, and hepatitis (11-14), although it also can suppress the replication of HIV (15). CCL3 additionally promotes hyperalgesia by sensitizing sensory neurons to TRPV1-mediated noxious stimulation (16).

References
  1. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
  2. Shi, M.M. et al. (1995) Biochem. Biophys. Res. Commun. 211:289.
  3. Graham, G.J. et al. (1990) Nature 344:442.
  4. Wagner, L. et al. (1998) Nature 391:908.
  5. Graham, G.J. et al. (1994) J. Biol. Chem. 269:4974.
  6. Ren, M. et al. (2010) EMBO J. 29:3952.
  7. Weber, M. et al. (2004) Mol. Biol. Cell 15:2492
  8. Taub, D.D. et al. (1993) Science 260:355.
  9. Bernardini, G. et al. (2008) Blood 111:3626.
  10. Lee, S.C. et al. (2000) J. Immunol. 164:3392.
  11. Wu, Y. et al. (2008) J. Immunol. 181:6384.
  12. Cook, D.N. et al. (1995) Science 269:1583.
  13. Chintalacharuvu, S.R. et al. (2005) Immunol. Lett. 100:202.
  14. Ajuebor, M.N. et al. (2004) Eur. J. Immunol. 34:2907.
  15. Cocchi, F. et al. (1995) Science 270:1811.
  16. Zhang, N. et al. (2005) Proc. Natl. Acad. Sci. 102:4536.
Entrez Gene IDs
6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine); 102136134 (Cynomolgus Monkey)
Alternate Names
C-C motif chemokine 3; MIP1-(a); AI323804; CCL3; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP1 alpha; MIP-1 alpha; MIP1A; MIP-1alpha; MIP1-alpha; PAT 464.1; SCYA3; SIS-beta

Product Datasheets

Citation for Rat CCL3/MIP-1 alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Involvement of the chemokine CCL3 and the purinoceptor P2X7 in the spinal cord in paclitaxel-induced mechanical allodynia.
    Authors: Ochi-ishi, Ryutaro, Nagata, Kenichir, Inoue, Tomoyuki, Tozaki-Saitoh, Hidetosh, Tsuda, Makoto, Inoue, Kazuhide
    Mol Pain, 2014;10(0):53.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

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