Rat CCL3/MIP-1 alpha Antibody Summary
Accession # P50229
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Rat CCL3/MIP‑1 alpha by Western Blot. Western blot shows lysates of NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Rat CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB66252) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CCL3/MIP-1a at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutral-ization by Rat CCL3/ MIP‑1 alpha Antibody. Recombinant Rat CCL3/MIP-1a (Catalog # 6625-MA) induces chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Rat CCL3/MIP-1a (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB66252). The ND50 is typically 0.075-0.45 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL3/MIP-1 alpha
CCL3, also known as macrophage inflammatory protein 1 alpha (MIP-1 alpha ) and LD78, is a member of the beta or CC subfamily of chemokines and is closely related to CCL4/MIP-1 beta. Chemokines comprise a large family of small secreted proteins that are involved in immune and inflammatory responses. CCL3 expression can be induced in a variety of hematopoietic cells, fibroblasts, smooth muscle cells, and epithelial cells (1). Mature rat CCL3 shares 74%, 91%, and 88% amino acid sequence identity with human, mouse, and cotton rat CCL3, respectively (2). CCL3 is an approximately 8 kDa chemokine that forms complexes with sulfated proteoglycans (3, 4). In a reversible process, CCL3 associates into noncovalently-linked dimers which then form tetramers and high molecular weight polymers (5, 6). These complexes of CCL3 are protected from proteolytic digestion by insulin degrading enzyme (IDE) which can cleave the monomeric chemokine (6). CCL3 exerts its biological functions through interactions with CCR1, CCR3, and CCR5 (1). It is cleared from the extracellular space by internalization via the decoy chemokine receptor D6 (7). CCL3 promotes the chemoattraction, adhesion to activated vascular endothelium, and cellular activation of many hematopoietic cell types including activated T cells, NK cells, neutrophils, monocytes, immature dendritic cells, and eosinophils (1, 8-10). CCL3 is also known as stem cell inhibitor (SCI) and can inhibit the proliferation of hematopoietic progenitor cells (3). CCL3 bioactivity contributes to tumor metastasis and the inflammatory components of viral infection, rheumatoid arthritis, and hepatitis (11-14), although it also can suppress the replication of HIV (15). CCL3 additionally promotes hyperalgesia by sensitizing sensory neurons to TRPV1-mediated noxious stimulation (16).
- Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
- Shi, M.M. et al. (1995) Biochem. Biophys. Res. Commun. 211:289.
- Graham, G.J. et al. (1990) Nature 344:442.
- Wagner, L. et al. (1998) Nature 391:908.
- Graham, G.J. et al. (1994) J. Biol. Chem. 269:4974.
- Ren, M. et al. (2010) EMBO J. 29:3952.
- Weber, M. et al. (2004) Mol. Biol. Cell 15:2492
- Taub, D.D. et al. (1993) Science 260:355.
- Bernardini, G. et al. (2008) Blood 111:3626.
- Lee, S.C. et al. (2000) J. Immunol. 164:3392.
- Wu, Y. et al. (2008) J. Immunol. 181:6384.
- Cook, D.N. et al. (1995) Science 269:1583.
- Chintalacharuvu, S.R. et al. (2005) Immunol. Lett. 100:202.
- Ajuebor, M.N. et al. (2004) Eur. J. Immunol. 34:2907.
- Cocchi, F. et al. (1995) Science 270:1811.
- Zhang, N. et al. (2005) Proc. Natl. Acad. Sci. 102:4536.
Citation for Rat CCL3/MIP-1 alpha Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Involvement of the chemokine CCL3 and the purinoceptor P2X7 in the spinal cord in paclitaxel-induced mechanical allodynia.
Authors: Ochi-ishi, Ryutaro, Nagata, Kenichir, Inoue, Tomoyuki, Tozaki-Saitoh, Hidetosh, Tsuda, Makoto, Inoue, Kazuhide
Mol Pain, 2014;10(0):53.
Sample Types: In Vivo
Applications: In Vivo
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Rat CCL3/MIP-1 alpha Antibody
There are currently no reviews for this product. Be the first to review Rat CCL3/MIP-1 alpha Antibody and earn rewards!
Have you used Rat CCL3/MIP-1 alpha Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image