Rat CNTF R alpha Biotinylated Antibody Summary
Accession # Q08406
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
CNTF R alpha in Rat Brain. CNTF R alpha was detected in perfusion fixed frozen sections of rat brain using 5 µg/mL Goat Anti-Rat CNTF R alpha Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF559) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CNTF R alpha
The high-affinity CNTF receptor complex, which mediates the biological action of CNTF, contains the ligand-binding alpha subunit (CNTF R alpha) and two signal-transducing proteins, LIF R beta and gp130. Whereas LIF R beta and gp130 are widely expressed in many cell types, the expression of CNTF R alpha is restricted to the central and peripheral nervous systems. cDNAs encoding CNTF R alpha isolated from both human and rat share 96% amino acid sequence identity.
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