Rat CNTF R alpha Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF559
CNTF R alpha  in Rat Brain.
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Rat CNTF R alpha Biotinylated Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat CNTF R alpha in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant rat CNTF R alpha
Ala19-Pro346
Accession # Q08406
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat CNTF R alpha (Catalog # 558-CR)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry CNTF R alpha  in Rat Brain. View Larger

CNTF R alpha in Rat Brain. CNTF R alpha was detected in perfusion fixed frozen sections of rat brain using 5 µg/mL Goat Anti-Rat CNTF R alpha Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF559) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CNTF R alpha

The high-affinity CNTF receptor complex, which mediates the biological action of CNTF, contains the ligand-binding alpha subunit (CNTF R alpha) and two signal-transducing proteins, LIF R beta and gp130. Whereas LIF R beta and gp130 are widely expressed in many cell types, the expression of CNTF R alpha is restricted to the central and peripheral nervous systems. cDNAs encoding CNTF R alpha isolated from both human and rat share 96% amino acid sequence identity.

Long Name
Ciliary Neurotrophic Factor Receptor alpha
Entrez Gene IDs
1271 (Human); 313173 (Rat)
Alternate Names
CNTF R alpha; CNTFR; CNTFRa

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Staining Reagents

Avidin-Fluorescein

Supplemental Cell Selection Products

Supplemental ELISA Products

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