CXCL1/GRO alpha/KC/CINC-1 is an ELR+ angiogenic chemokine that interacts with CXCR2/IL-8 RB to attract and activate neutrophils. It is produced at sites of microbial infection by macrophages, monocytes, Kupffer cells, hepatocytes, endothelial and epithelial cells, cardiac and vascular smooth muscle cells, fibroblasts, melanocytes, mast cells, and neurons. CXCL1 function is associated with the development and extent of atherosclerosis, Alzheimer’s disease, and tumor angiogenesis.
Rat CXCL1/CINC-1 Quantikine ELISA Kit
R&D Systems | Catalog # RCN100
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)
Sensitivity
1.3 pg/mL
Assay Range
7.8-500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Rat CXCL1/CINC-1 Quantikine ELISA Kit
The Quantikine Rat CINC-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure rat CINC-1 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat CINC-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural rat CINC-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring rat CINC-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Rat
Specificity
Natural and recombinant rat CINC-1
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 23 | 23 | 23 |
| Mean (pg/mL) | 53 | 71 | 215 | 49 | 71 | 190 |
| Standard Deviation | 2.7 | 5.2 | 12 | 3.1 | 3.3 | 12 |
| CV% | 5.1 | 7.3 | 5.6 | 6.3 | 4.6 | 6.3 |
Recovery for Rat CXCL1/CINC-1 Quantikine ELISA Kit
The recovery of rat CINC-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 99 | 93-106 |
| EDTA Plasma (n=4) | 97 | 90-104 |
| Heparin Plasma (n=4) | 99 | 90-113 |
| Serum (n=5) | 95 | 89-103 |
Linearity
To assess the linearity of the assay, samples containing rat CINC-1 were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Rat CXCL1/CINC-1 Quantikine ELISA Kit
Rat CXCL1/GRO alpha/KC/CINC-1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL1/GRO alpha/KC/CINC-1
Alternate Names
CINC-1, CINC1, GRO alpha, KC, MGSA-alpha
Gene Symbol
CXCL1
Additional CXCL1/GRO alpha/KC/CINC-1 Products
Product Documents for Rat CXCL1/CINC-1 Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Rat CXCL1/CINC-1 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Rat CXCL1/CINC-1 Quantikine ELISA Kit
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Customer Reviews for Rat CXCL1/CINC-1 Quantikine ELISA Kit (3)
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3 Customer Ratings
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Sample Tested: SerumVerified Customer | Posted 05/17/2023
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Sample Tested: Recombinant proteinVerified Customer | Posted 10/26/2021
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Sample Tested: Serum and PlasmaVerified Customer | Posted 08/18/2021The amount of CINC-1 was measured using 5-week-old rat serum. The results showed that the background was very low and the amount of CINC-1 could be quantified with high accuracy. The kit was very accurate in quantitating CINC-1 with very low background and quicker than other kits.
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Protocols
View specific protocols for Rat CXCL1/CINC-1 Quantikine ELISA Kit (RCN100):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
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- How to Run an R&D Systems Quantikine ELISA
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- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
