Rat CXCL1/CINC-1 Quantikine ELISA Kit

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RCN100
Rat CXCL1/GRO alpha/KC/CINC-1 ELISA Standard Curve
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Rat CXCL1/CINC-1 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity
1.3 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant rat CINC-1
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Rat CINC-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure rat CINC-1 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat CINC-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural rat CINC-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring rat CINC-1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 23 23 23
Mean 53 71 215 49 71 190
Standard Deviation 2.7 5.2 12 3.1 3.3 12
CV% 5.1 7.3 5.6 6.3 4.6 6.3

Recovery

The recovery of rat CINC-1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 99 93-106
EDTA Plasma (n=4) 97 90-104
Heparin Plasma (n=4) 99 90-113
Serum (n=5) 95 89-103

Linearity

To assess the linearity of the assay, samples containing rat CINC-1 were diluted with Calibrator Diluent and assayed.
Rat CXCL1/GRO alpha/KC/CINC-1 ELISA Linearity

Data Examples

Rat CXCL1/GRO alpha/KC/CINC-1 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL1/GRO alpha/KC/CINC-1

CXCL1/GRO alpha, also known as KC in mouse and CINC-1 in rat, is a member of the CXC family of chemokines. Human CXCL1/GRO alpha is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 57% and 59% amino acid sequence identity with the human protein, respectively. Genome-wide analysis suggests that the protein encoded by the mouse Cxcl3 gene is homologous to human CXCL1/GRO alpha protein. Human CXCL1/GRO alpha can be cleaved into three isoforms CXCL1/GRO alpha (aa 4-73), CXCL1/GRO alpha (aa 5-73), and CXCL1/GRO alpha (aa 6-73) which show higher activity than the full length protein. CXCL1/GRO alpha is secreted by mast cells and macrophages whereupon it acts as a chemoattractant for neutrophils.

Entrez Gene IDs:
2919 (Human); 14825 (Mouse); 81503 (Rat)
Alternate Names:
CINC1; CINC-1; CXCL1; GRO alpha; KC; MGSA-alpha
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Rat CXCL1/CINC-1 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Dexamethasone-induced Intra-Uterine Growth Restriction impacts NOSTRIN and its downstream effector genes in the rat mesometrial uterus
    Authors: S Chakrabort, S Islam, S Saha, R Ain
    Sci Rep, 2018;8(1):8342.
    Species: Rat
    Sample Types: Tissue Homogenates
  2. Assessment of Pulmonary Toxicity Induced by Inhaled Toner with External Additives
    Authors: T Tomonaga, H Izumi, Y Yoshiura, T Myojo, T Oyabu, BW Lee, T Okada, Y Li, K Kawai, T Higashi, Y Morimoto
    Biomed Res Int, 2017;2017(0):4245309.
    Species: Mouse
    Sample Types: Tissue Homogenates
  3. [EXPRESS] The Chemokine Receptor CXCR2 Supports Nociceptive Sensitization after Traumatic Brain Injury
    Authors: DY Liang, X Shi, P Liu, Y Sun, P Sahbaie, WW Li, DC Yeomans, JD Clark
    Mol Pain, 2017;13(0):1744806917730.
    Species: Rat
    Sample Types: Tissue Homogenates
  4. The mitochondrial inhibitor oligomycin induces an inflammatory response in the rat knee joint
    Authors: C Vaamonde-G, J Loureiro, MN Valcárcel-, RR Riveiro-Na, O Ramil-Góme, L Hermida-Ca, A Centeno, R Meijide-Fa, FJ Blanco, MJ López-Arma
    BMC Musculoskelet Disord, 2017;18(1):254.
    Species: Rat
    Sample Types: Tissue Homogenates
  5. The extent of irradiation-induced long-term visceral organ damage depends on cranial/brain exposure.
    Authors: Boittin F, Denis J, Mayol J, Martigne P, Raffin F, Coulon D, Grenier N, Drouet M, Herodin F
    PLoS ONE, 2015;10(4):e0122900.
    Species: Rat
    Sample Types: Plasma
  6. Effect of Silodosin, an Alpha1A-Adrenoceptor Antagonist, on Ventral Prostatic Hyperplasia in the Spontaneously Hypertensive Rat.
    Authors: Shimizu S, Shimizu T, Tsounapi P, Higashi Y, Martin D, Nakamura K, Honda M, Inoue K, Saito M
    PLoS ONE, 2015;10(8):e0133798.
    Species: Rat
    Sample Types: Tissue Homogenates
  7. NF-kappaB and STAT1 control CXCL1 and CXCL2 gene transcription.
    Authors: Burke S, Lu D, Sparer T, Masi T, Goff M, Karlstad M, Collier J
    Am J Physiol Endocrinol Metab, 2014;306(2):E131-49.
    Species: Rat
    Sample Types: Cell Culture Supernates
  8. Medium-chain fatty acid-sensing receptor, GPR84, is a proinflammatory receptor.
    Authors: Suzuki M, Takaishi S, Nagasaki M, Onozawa Y, Iino I, Maeda H, Komai T, Oda T
    J Biol Chem, 2013;288(15):10684-91.
    Species: Rat
    Sample Types: Serum
  9. Nebulized hypertonic saline attenuates acute lung injury following trauma and hemorrhagic shock via inhibition of matrix metalloproteinase-13.
    Authors: Wohlauer M, Moore E, Silliman C, Fragoso M, Gamboni F, Harr J, Accurso F, Wright F, Haenel J, Fullerton D, Banerjee A
    Crit Care Med, 2012;40(9):2647-53.
    Species: Rat
    Sample Types: BALF
  10. Xanthine oxidoreductase promotes the inflammatory state of mononuclear phagocytes through effects on chemokine expression, peroxisome proliferator-activated receptor-gamma sumoylation, and HIF-1alpha.
    Authors: Gibbings S, Elkins ND, Fitzgerald H, Tiao J, Weyman ME, Shibao G, Fini MA, Wright RM
    J. Biol. Chem., 2011;286(2):961-75.
    Species: Rat
    Sample Types: BALF
  11. Plasma from stored packed red blood cells and MHC class I antibodies causes acute lung injury in a 2-event in vivo rat model.
    Authors: Kelher MR, Masuno T, Moore EE, Damle S, Meng X, Song Y, Liang X, Niedzinski J, Geier SS, Khan SY, Gamboni-Robertson F, Silliman CC
    Blood, 2009;113(9):2079-87.
    Species: Rat
    Sample Types: BALF
  12. Mechanism of hepatoprotection in proestrus female rats following trauma-hemorrhage: heme oxygenase-1-derived normalization of hepatic inflammatory responses.
    Authors: Yang S, Hu S, Chen J, Choudhry MA, Rue LW, Bland KI, Chaudry IH
    J. Leukoc. Biol., 2009;85(6):1015-26.
    Species: Rat
    Sample Types: Serum
  13. The compatible solute ectoine protects against nanoparticle-induced neutrophilic lung inflammation.
    Authors: Sydlik U, Gallitz I, Albrecht C, Abel J, Krutmann J, Unfried K
    Am. J. Respir. Crit. Care Med., 2009;180(1):29-35.
    Species: Rat
    Sample Types: BALF
  14. Alterations in the proteome of pulmonary alveolar type II cells in the rat after hepatic ischemia-reperfusion.
    Authors: Hirsch J, Niemann CU, Hansen KC, Choi S, Su X, Frank JA, Fang X, Hirose R, Theodore P, Sapru A, Burlingame AL, Matthay MA
    Crit. Care Med., 2008;36(6):1846-54.
    Species: Rat
    Sample Types: Plasma
  15. The arthritis severity quantitative trait loci Cia4 and Cia6 regulate neutrophil migration into inflammatory sites and levels of TNF-alpha and nitric oxide.
    Authors: Laragione T, Yarlett NC, Brenner M, Mello A, Sherry B, Miller EJ, Metz CN, Gulko PS
    J. Immunol., 2007;178(4):2344-51.
    Species: Rat
    Sample Types: Air Pouch Exudate
  16. Mechanism of the nongenomic effects of estrogen on intestinal myeloperoxidase activity following trauma-hemorrhage: up-regulation of the PI-3K/Akt pathway.
    Authors: Yu HP, Hsieh YC, Suzuki T, Choudhry MA, Schwacha MG, Bland KI, Chaudry IH
    J. Leukoc. Biol., 2007;82(3):774-80.
    Species: Rat
    Sample Types: Tissue Homogenates
  17. Anti-rat soluble IL-6 receptor antibody down-regulates cardiac IL-6 and improves cardiac function following trauma-hemorrhage.
    Authors: Yang S, Hu S, Choudhry MA, Rue LW, Bland KI, Chaudry IH
    J. Mol. Cell. Cardiol., 2007;42(3):620-30.
    Species: Rat
    Sample Types: Tissue Homogenates
  18. Immunotargeting of catalase to lung endothelium via anti-angiotensin-converting enzyme antibodies attenuates ischemia-reperfusion injury of the lung in vivo.
    Authors: Nowak K, Weih S, Metzger R, Albrecht RF, Post S, Hohenberger P, Gebhard MM, Danilov SM
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(1):L162-9.
    Species: Rat
    Sample Types: Serum
  19. Maintenance of lung myeloperoxidase activity in proestrus females after trauma-hemorrhage: upregulation of heme oxygenase-1.
    Authors: Yu HP, Yang S, Hsieh YC, Choudhry MA, Bland KI, Chaudry IH
    Am. J. Physiol. Lung Cell Mol. Physiol., 2006;291(3):L400-6.
    Species: Rat
    Sample Types: Tissue Homogenates
  20. Mechanism of IL-6-mediated cardiac dysfunction following trauma-hemorrhage.
    Authors: Yang S, Hu S, Hsieh YC, Choudhry MA, Rue LW, Bland KI, Chaudry IH
    J. Mol. Cell. Cardiol., 2006;40(4):570-9.
    Species: Rat
    Sample Types: Tissue Homogenates

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