Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Mouse, Avian - Chicken

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all GFR alpha-1/GDNF R alpha-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat GFR alpha ‑1/GDNF R alpha ‑1
Asp25-Leu445
Accession # Q62997

Specificity

Detects rat GFR alpha ‑1/GDNF R alpha ‑1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat GFR alpha-1/GDNF R alpha-1 Biotinylated Antibody

GDNF and GFRa‑1 antibody in Rat Dorsal Root Ganglia by Immunohistochemistry (IHC-Fr).

GDNF and GFR alpha ‑1 in Rat Dorsal Root Ganglia.

GDNF and GFRa-1 were detected in perfusion fixed frozen sections of rat dorsal root ganglia using 10 µg/mL Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF-212-NA) and 15 µg/mL Goat AntiRat Anti-GFRa-1 Biotinylated Antigen Affinity-purified Poly-clonal Antibody (red; Catalog # BAF560). Tissue was incubated with primary antibodies overnight at 4 °C. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Rat GFR alpha-1/GDNF R alpha-1 Biotinylated Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (cortex) and rat dorsal root ganglia

Western Blot

0.1 µg/mL
Sample: Recombinant Rat GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 560-GR)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GFR alpha-1/GDNF R alpha-1

Glial cell line-derived growth factor (GDNF), neurturin (NTN) and persephin, distant members of the TGF-beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha - (GFR alpha -1) or GFR alpha -2). GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase. Rat GFR alpha -1 cDNA encodes a 468 amino acid (aa) residue protein with an N‑terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% amino acid identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

References

  1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
  2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
  3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.

Long Name

Glial Cell line-derived Neurotrophic Factor Receptor alpha 1

Alternate Names

GDNF R alpha-1, GFR alpha1, GFRa-1, GFRA1

Entrez Gene IDs

2674 (Human); 14585 (Mouse); 25454 (Rat)

Gene Symbol

GFRA1

UniProt

Q62997

Additional GFR alpha-1/GDNF R alpha-1 Products

Product Documents for Rat GFR alpha-1/GDNF R alpha-1 Biotinylated Antibody

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Product Specific Notices for Rat GFR alpha-1/GDNF R alpha-1 Biotinylated Antibody

For research use only

Related Research Areas

GDNF Family

Citations for Rat GFR alpha-1/GDNF R alpha-1 Biotinylated Antibody

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Protocols

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