Rat IFN-gamma Antibody

Catalog # Availability Size / Price Qty
MAB585-SP
MAB585-500
MAB585-100
IFN‑ gamma  Inhibition of EMCV-induced Cytopathy and Neutralization by Rat IFN‑ gamma  Antibody.
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Rat IFN-gamma Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat IFN-gamma in Western blots. In Western blots, this antibody shows less than 1% cross-reactivity with recombinant mouse (rm) IFN‑ gamma and no cross-reactivity with rhIFN‑ gamma.
Source
Monoclonal Mouse IgG1 Clone # 88915
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant rat IFN-gamma
Gln23-Cys156
Accession # P01581
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Rat IFN-gamma (Catalog # 585-IF)
Neutralization
Measured by its ability to neutralize IFN‑ gamma inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. Vogel, S. and M. Hogan (1995) in Current Protocols in Immunology. Ciocio, R. (ed); John Wiley & Sons, Inc. p. 6. 9. 1. The Neutralization Dose (ND50) is typically 0.5-1.5 µg/mL in the presence of 2.5 ng/mL Recombinant Rat IFN‑ gamma.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization IFN‑ gamma  Inhibition of EMCV-induced Cytopathy and Neutralization by Rat IFN‑ gamma  Antibody. View Larger

IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Rat IFN‑ gamma Antibody. Recombinant Rat IFN-gamma (Catalog # 585-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Rat IFN-gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat IFN-gamma Monoclonal Antibody (Catalog # MAB585). The ND50 is typically 0.5-1.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature rat IFN-gamma exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 86% amino acid sequence identity with mouse IFN-gamma and 37-45% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

References
  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Dijkema, R. et al. (1985) EMBO J. 4:761.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. USA 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
Long Name
Interferon gamma
Entrez Gene IDs
3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Rat IFN-gamma Antibody
By Anonymous on 11/08/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Rat

This antibody was used to develop and ELISA to measure rat IFNy in serum and plasma samples. This antibody was paired with BAF585 in a sandwich ELISA format.