Rat IFN-gamma Quantikine ELISA Kit

  (24 citations)     
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Product Details
Assay Procedure
Citations (24)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Specificity
    Natural and recombinant rat IFN-gamma
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Rat IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IFN-gamma levels in cell culture supernates and serum. It contains E. coli-expressed recombinant rat IFN-gamma and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant rat IFN-gamma accurately. Results obtained using natural rat IFN-gamma showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IFN-gamma.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 86.9 215 1159 82.8 205 1135
Standard Deviation 3.5 4.4 23 8 14.5 48
CV% 4 2 2 9.7 7.1 4.2

Recovery

The recovery of rat IFN-gamma spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 108 89-119
Serum (n=6) 100 89-111
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of rat IFN-gamma in each matrix were diluted with Calibrator Diluent and then assayed.
 IFN-gamma [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IFN-gamma
IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.
  • Long Name:
    Interferon gamma
  • Entrez Gene IDs:
    3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
  • Alternate Names:
    IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 120 µL Conjugate
  9.   Add 120 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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Species
Sample Type
  1. Novel protective role of curcumin and taurine combination against experimental hepatocarcinogenesis
    Authors: ME El-Housein, IA El-Agoza, MM Sakr, GM El-Malky
    Exp Ther Med, 2017;13(1):29-36.
    Species: Rat
    Sample Type: Serum
  2. Topical use and systemic action of green and roasted coffee oils and ground oils in a cutaneous incision model in rats (Rattus norvegicus albinus)
    Authors: BG Lania, J Morari, AL Souza, MND Silva, AR de Almeida, G Veira-Dami, SM Alegre, CL César, LA Velloso, ML Cintra, NB Maia, PENF Velho
    PLoS ONE, 2017;12(12):e0188779.
    Species: Rat
    Sample Type: Serum
  3. Mori Folium water extract alleviates articular cartilage damages and inflammatory responses in monosodium iodoacetate?induced osteoarthritis rats
    Authors: JW Jeong, HH Lee, J Kim, EO Choi, H Hwang-Bo, HJ Kim, MY Kim, KI Ahn, GY Kim, KW Lee, KY Kim, SG Kim, SH Hong, C Park, HJ Cha, YH Choi
    Mol Med Rep, 2017;0(0):.
    Species: Rat
    Sample Type: Serum
  4. Biological effects of bone marrow mesenchymal stem cells on hepatitis B virus in�vitro
    Authors: WP Zheng, BY Zhang, ZY Shen, ML Yin, Y Cao, HL Song
    Mol Med Rep, 2017;15(5):2551-2559.
    Species: Rat
    Sample Type: Cell Culture Supernates
  5. Potassium channels Kv1.3 and KCa3.1 cooperatively and compensatorily regulate antigen-specific memory T cell functions
    Authors: EY Chiang, T Li, S Jeet, I Peng, J Zhang, WP Lee, J DeVoss, P Caplazi, J Chen, S Warming, DH Hackos, S Mukund, CM Koth, JL Grogan
    Nat Commun, 2017;8(0):14644.
    Species: Rat
    Sample Type: Cell Culture Supernates
  6. Iron-hepcidin dysmetabolism, anemia and renal hypoxia, inflammation and fibrosis in the remnant kidney rat model.
    Authors: Garrido P, Ribeiro S, Fernandes J, Vala H, Bronze-da-Rocha E, Rocha-Pereira P, Belo L, Costa E, Santos-Silva A, Reis F
    PLoS ONE, 2015;10(4):e0124048.
    Species: Rat
    Sample Type: Serum
  7. Melatonin attenuates the TLR4-mediated inflammatory response through MyD88- and TRIF-dependent signaling pathways in an in vivo model of ovarian cancer.
    Authors: Chuffa L, Fioruci-Fontanelli B, Mendes L, Ferreira Seiva F, Martinez M, Favaro W, Domeniconi R, Pinheiro P, Delazari Dos Santos L, Martinez F
    BMC Cancer, 2015;15(0):34.
    Species: Rat
    Sample Type: Tissue Culture Supernates
  8. Oridonin promotes CD4+/CD25+ Treg differentiation, modulates Th1/Th2 balance and induces HO-1 in rat splenic lymphocytes.
    Authors: Hu AP, Du JM, Li JY, Liu JW
    Inflamm. Res., 2008;57(4):163-70.
    Species: Rat
    Sample Type: Cell Culture Supernates
  9. Changes of gene expression of iron regulatory proteins during turpentine oil-induced acute-phase response in the rat.
    Authors: Sheikh N, Dudas J, Ramadori G
    Lab. Invest., 2007;87(7):713-25.
    Species: Rat
    Sample Type: Serum
  10. Comprehensive gene expression profiling of rat lung reveals distinct acute and chronic responses to cigarette smoke inhalation.
    Authors: Stevenson CS, Docx C, Webster R, Battram C, Hynx D, Giddings J, Cooper PR, Chakravarty P, Rahman I, Marwick JA, Kirkham PA, Charman C, Richardson DL, Nirmala NR, Whittaker P, Butler K
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(5):L1183-93.
    Species: Rat
    Sample Type: BALF
  11. The age-related attenuation in long-term potentiation is associated with microglial activation.
    Authors: Griffin R, Nally R, Nolan Y, McCartney Y, Linden J, Lynch MA
    J. Neurochem., 2006;99(4):1263-72.
    Species: Rat
    Sample Type: Tissue Homogenates
  12. Suppression of autoimmune retinal disease by lovastatin does not require Th2 cytokine induction.
    Authors: Gegg ME, Harry R, Hankey D, Zambarakji H, Pryce G, Baker D, Adamson P, Calder V, Greenwood J
    J. Immunol., 2005;174(4):2327-35.
    Species: Rat
    Sample Type: Cell Culture Supernates
  13. Immunotherapy of murine prostate cancer using whole tumour cells killed ex vivo by cytosine deaminase/5-fluorocytosine suicide-gene therapy.
    Authors: Pandha HS, Cook D, Greenhalgh R, Dalgleish A
    BJU Int., 2005;95(9):1336-43.
    Species: Rat
    Sample Type: Cell Culture Supernates
  14. Interleukin-10 aerosol reduces proinflammatory mediators in bronchoalveolar fluid of endotoxemic rat.
    Authors: Hofstetter C, Flondor M, Hoegl S, Muhl H, Zwissler B
    Crit. Care Med., 2005;33(10):2317-22.
    Species: Rat
    Sample Type: BALF
  15. Effects of pyrrolidine dithiocarbamate, an NF-kappaB inhibitor, on cytokine expression and ocular inflammation in experimental autoimmune anterior uveitis.
    Authors: Fang IM, Yang CH, Lin CP, Yang CM, Chen MS
    J Ocul Pharmacol Ther, 2005;21(2):95-106.
    Species: Rat
    Sample Type: Aqueous humor
  16. Ameliorating effects of the immunomodulator 3-(2-ethylphenyl)-5-(3-methoxyphenyl)-1H-1,2,4-triazole in an experimental model of colitis in the rat.
    Authors: Stasi MA, Ruggiero V, Ursillo A, Taurelli R, Agliano M, Weber E, Lorenzoni P, Sorrentino V, Pacifici L, Carminati P
    Eur. J. Pharmacol., 2004;494(2):263-72.
    Species: Rat
    Sample Type: Tissue Homogenates
  17. Salivary gland genetic vaccination: a scalable technology for promoting distal mucosal immunity and heightened systemic immune responses.
    Authors: Tucker SN, Lin K, Stevens S, Scollay R, Bennett MJ, Olson DC
    Vaccine, 2004;22(19):2500-4.
    Species: Rat
    Sample Type: Cell Culture Supernates
  18. Peroxisome proliferator-activated receptor-gamma ligands ameliorate experimental autoimmune myocarditis associated with inhibition of self-sensitive T cells.
    Authors: Yuan Z, Liu Y, Liu Y, Zhang J, Kishimoto C, Ma A, Liu Z
    J. Cardiovasc. Pharmacol., 2004;43(6):868-75.
    Species: Rat
    Sample Type:
  19. Gene transfer of tumor necrosis factor inhibitor improves the function of lung allografts.
    Authors: Tagawa T, Kozower BD, Kanaan SA, Daddi N, Muraoka M, Oka T, Ritter JH, Patterson GA
    J. Thorac. Cardiovasc. Surg., 2004;127(6):1558-63.
    Species: Rat
    Sample Type: Tissue Homogenates
  20. Alveolar macrophage cytotoxicity for normal lung fibroblasts is mediated by nitric oxide release.
    Authors: Morgan DL, Shines CJ
    Toxicol In Vitro, 2004;18(1):139-46.
    Species: Rat
    Sample Type: Cell Culture Supernates
  21. Recipient intramuscular cotransfection of naked plasmid transforming growth factor beta1 and interleukin 10 ameliorates lung graft ischemia-reperfusion injury.
    Authors: D&amp;amp;apos;Ovidio F
    J. Thorac. Cardiovasc. Surg., 2002;124(2):259-69.
    Species: Rat
    Sample Type: BALF
  22. Melatonin fails to modulate immune parameters influenced by calorie restriction in aging Fischer 344 rats.
    Authors: Pahlavani MA, Vargas DA, Evans TR, Shu JH, Nelson JF
    Exp. Biol. Med. (Maywood), 2002;227(3):201-7.
    Species: Rat
    Sample Type: Cell Culture Supernates
  23. Chimeric smooth muscle-specific enhancer/promoters: valuable tools for adenovirus-mediated cardiovascular gene therapy.
    Authors: Ribault S, Neuville P, Mechine-Neuville A, Auge F, Parlakian A, Gabbiani G, Paulin D, Calenda V
    Circ. Res., 2001;88(5):468-75.
    Species: Rat
    Sample Type: Cell Culture Supernates
  24. Role of IL-18 in acute lung inflammation.
    Authors: Jordan JA, Guo RF, Yun EC, Sarma V, Warner RL, Crouch LD, Senaldi G, Ulich TR, Ward PA
    J. Immunol., 2001;167(12):7060-8.
    Species: Rat
    Sample Type: BALF
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