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Rat IL-6 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
125.0 - 8,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant rat IL-6. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008B) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Block Buffer: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Reagent Diluent: 0.1% BSA, 0.05% Tween 20 in Tris-buffered Saline (20 mM Trizma base, 150 mM NaCI) pH 7.2-7.4, 0.2 μm filtered

Substrate Solution: TMB ELISA Substrate (Catalog # DY999B)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

 

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product.

Data Example

Rat IL-6 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-6

IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.

Long Name:
Interleukin 6
Entrez Gene IDs:
3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names:
B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor ; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent with NGS, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Rat IL-6 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

78 Citations: Showing 1 - 10
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  1. The evaluation of cytotoxicity and cytokine IL-6 production of root canal sealers with and without the incorporation of simvastatin: an invitro study
    Authors: A Sharma, K Sanjeev, VMJ Selvanatha, M Sekar, N Harikrishn
    BMC Oral Health, 2022;22(1):6.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. Exposure to Nickel Oxide Nanoparticles Induces Acute and Chronic Inflammatory Responses in Rat Lungs and Perturbs the Lung Microbiome
    Authors: MJ Jeong, S Jeon, HS Yu, WS Cho, S Lee, D Kang, Y Kim, YJ Kim, SY Kim
    International Journal of Environmental Research and Public Health, 2022;19(1):.
    Species: Rat
    Sample Types: BALF
  3. In Vitro Evaluation of the Anti-Inflammatory Effect of KMUP-1 and In Vivo Analysis of Its Therapeutic Potential in Osteoarthritis
    Authors: SE Huang, E Sulistyowa, YY Chao, BN Wu, ZK Dai, JH Hsu, JL Yeh
    Biomedicines, 2021;9(6):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  4. Programming of intestinal homeostasis in male rat offspring after maternal exposure to chlorpyrifos and/or to a high fat diet
    Authors: M Guibourden, H El Khayat, N Djekkoun, H Khorsi-Cau, V Bach, PM Anton, J Gay-Quéhei
    Scientific Reports, 2021;11(1):11420.
    Species: Rat
    Sample Types: Plasma
  5. Co-Incubation with PPAR&beta/&delta Agonists and Antagonists Modeled Using Computational Chemistry: Effect on LPS Induced Inflammatory Markers in Pulmonary Artery
    Authors: N Perez Diaz, LA Lione, V Hutter, LS Mackenzie
    International Journal of Molecular Sciences, 2021;22(6):.
    Species: Rat
    Sample Types: Tissue Homogenates
  6. Local Immune Changes in Early Stages of Inflammation and Carcinogenesis Correlate with the Collagen Scaffold Changes of the Colon Mucosa
    Authors: F ?aja, D Stakheev, O Chernyavsk, L Kubinová, J K?ížan, J Dvo?ák, P Rossmann, R Št?pánková, P Makovický, P Makovický, V Vymetalkov, P Sou?ek, P Vodi?ka, L Vodi?ková, M Levý, LE Vannucci
    Cancers, 2021;13(10):.
    Species: Rat
    Sample Types: Tissue Homogenates
  7. TLR2- and TLR3-activated microglia induce different levels of neuronal network dysfunction in a context-dependent manner
    Authors: S Schilling, B Chausse, HO Dikmen, F Almouhanna, JO Hollnagel, A Lewen, O Kann
    Brain, Behavior, and Immunity, 2021;0(0):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  8. Mast cells contribute to alveolar bone loss in Spontaneously Hypertensive Rats with periodontal disease regulating cytokines production
    Authors: VGB Brito, MS Patrocinio, MCL Sousa, AEA Barreto, SCT Frasnelli, VS Lara, CF Santos, SHP Oliveira
    PLoS ONE, 2021;16(3):e0247372.
    Species: Rat
    Sample Types: Tissue Homogenates
  9. Inflammatory Cytokine Profile and Plasticity of Brain and Spinal Microglia in Response to ATP and Glutamate
    Authors: SJB Jesudasan, SJ Gupta, MA Churchward, KG Todd, IR Winship
    Frontiers in Cellular Neuroscience, 2021;15(0):634020.
    Species: Rat
    Sample Types: Cell Culture Supernates
  10. Small intestine remodeling in male Goto-Kakizaki rats
    Authors: JNB Pereira, GM Murata, FT Sato, AR Marosti, CRO Carvalho, R Curi
    Physiological Reports, 2021;9(3):e14755.
    Species: Rat
    Sample Types: Tissue Homogenates
  11. Local Immune Changes in Early Stages of Inflammation and Carcinogenesis Correlate with the Collagen Scaffold Changes of the Colon Mucosa
    Authors: F ?aja, D Stakheev, O Chernyavsk, L Kubinová, J K?ížan, J Dvo?ák, P Rossmann, R Št?pánková, P Makovický, P Makovický, V Vymetalkov, P Sou?ek, P Vodi?ka, L Vodi?ková, M Levý, LE Vannucci
    Cancers, 2021;13(10):.
    Species: Rat
    Sample Types: Tissue Homogenates
  12. Neuron-Derived Extracellular Vesicles Modulate Microglia Activation and Function
    Authors: H Peng, BT Harvey, CI Richards, K Nixon
    Biology, 2021;10(10):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  13. Moderate Physical Exercise Activates ATR2 Receptors, Improving Inflammation and Oxidative Stress in the Duodenum of 2K1C Hypertensive Rats
    Authors: ACA da Silva, JS Severo, BLB Dos Santos, PHM Mendes, LMS Nobre, AP de Oliveir, FCS Ferreira, JVR Medeiros, RC Lima-Junio, A Havt, RC Palheta-Ju, AA Dos Santos, M Tolentino
    Frontiers in Physiology, 2021;12(0):734038.
    Species: Rat
    Sample Types: Tissue Homogenates
  14. Effect of Pulmonary Inflammation by Surface Functionalization of Zinc Oxide Nanoparticles
    Authors: A Jung, SH Kim, JY Yang, J Jeong, JK Lee, JH Oh, JH Lee
    Toxics, 2021;9(12):.
    Species: Rat
    Sample Types: BALF
  15. Prenatal administration of IL-1Ra attenuate the neurodevelopmental impacts following non-pathogenic inflammation during pregnancy
    Authors: ME Brien, K Hughes, S Girard
    Scientific Reports, 2021;11(1):23404.
    Species: Rat
    Sample Types: Placenta
  16. The Prophylactic Effects of Glutamine on Muscle Protein Synthesis and Degradation in Rats with Ethanol-Induced Liver Damage
    Authors: Q Xiao, YH Chen, SA Pratama, YL Chen, H Shirakawa, HC Peng, SC Yang
    Nutrients, 2021;13(8):.
    Species: Rat
    Sample Types: Tissue Homogenates
  17. The Poststroke Peripheral Immune Response Is Differentially Regulated by Leukemia Inhibitory Factor in Aged Male and Female Rodents
    Authors: SM Davis, LA Collier, SJ Messmer, KR Pennypacke
    Oxidative Medicine and Cellular Longevity, 2020;2020(0):8880244.
    Species: Rat
    Sample Types: Tissue Homogenates
  18. Acute sleep deprivation during pregnancy in rats: Rapid elevation of placental and fetal inflammation and kynurenic acid
    Authors: AM Baratta, NR Kanyuch, CA Cole, H Valafar, J Deslaurier, A Pocivavsek
    Neurobiol Stress, 2020;12(0):100204.
    Species: Rat
    Sample Types: Tissue Homogenates
  19. GM-CSF induces noninflammatory proliferation of microglia and disturbs electrical neuronal network rhythms in situ
    Authors: HO Dikmen, M Hemmerich, A Lewen, JO Hollnagel, B Chausse, O Kann
    J Neuroinflammation, 2020;17(1):235.
    Species: Rat
    Sample Types: Whole Tissue
  20. Hepcidin-to-Ferritin Ratio Is Decreased in Astrocytes With Extracellular Alpha-Synuclein and Iron Exposure
    Authors: J Cui, X Guo, Q Li, N Song, J Xie
    Front Cell Neurosci, 2020;14(0):47.
    Species: Rat
    Sample Types: Cell Culture Supernates
  21. &alpha-Bisabolol, a Dietary Bioactive Phytochemical Attenuates Dopaminergic Neurodegeneration through Modulation of Oxidative Stress, Neuroinflammation and Apoptosis in Rotenone-Induced Rat Model of Parkinson's disease
    Authors: H Javed, MFN Meeran, S Azimullah, L Bader Eddi, VD Dwivedi, NK Jha, S Ojha
    Biomolecules, 2020;10(10):.
    Species: Rat
    Sample Types: Tissue Homogenates
  22. Fecal dysbiosis associated with colonic hypersensitivity and behavioral alterations in chronically Blastocystis-infected rats
    Authors: M Defaye, C Nourrisson, E Baudu, A Lashermes, M Meynier, M Meleine, I Wawrzyniak, V Bonnin, J Barbier, B Chassaing, C Godfraind, A Gelot, N Barnich, D Ardid, M Bonnet, F Delbac, FA Carvalho, P Poirier
    Sci Rep, 2020;10(1):9146.
    Species: Rat
    Sample Types: Tissue Homogenates
  23. Impacts of fish oil on the gut microbiota of rats with alcoholic liver damage
    Authors: YL Chen, H Shirakawa, NS Lu, HC Peng, Q Xiao, SC Yang
    J. Nutr. Biochem., 2020;86(0):108491.
    Species: Rat
    Sample Types: Cell Culture Supernates
  24. Time dependent dual effect of anti-inflammatory treatments on sarin-induced brain inflammation: suggested role of prostaglandins
    Authors: S Chapman, E Grauer, R Gez, I Egoz, S Lazar
    Neurotoxicology, 2019;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  25. Metabolomics Approach Based on Multivariate Techniques for Blood Transfusion Reactions
    Authors: SJ Lee, H Wang, SH Ahn, MK Son, GH Hyun, SJ Yoon, J Lee, JH Park, J Lim, SS Hong, SW Kwon
    Sci Rep, 2019;9(1):1740.
    Species: Rat
    Sample Types: Serum
  26. BRD4 inhibition attenuates inflammatory response in microglia and facilitates recovery after spinal cord injury in rats
    Authors: J Wang, J Chen, H Jin, D Lin, Y Chen, X Chen, B Wang, S Hu, Y Wu, Y Wu, Y Zhou, N Tian, W Gao, X Wang, X Zhang
    J. Cell. Mol. Med., 2019;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  27. Cholinergic Stimulation by Pyridostigmine Bromide Before Myocardial Infarction Prevent Cardiac and Autonomic Dysfunction
    Authors: CA Barboza, AR Fukushima, N Carrozzi, JF Machi, PMM Dourado, CT Mostarda, MC Irigoyen, L Nathanson, M Morris, EC Caperuto, B Rodrigues
    Sci Rep, 2019;9(1):2481.
    Species: Rat
    Sample Types: Plasma
  28. Matrix-bound nanovesicles prevent ischemia-induced retinal ganglion cell axon degeneration and death and preserve visual function
    Authors: Y van der Me, AE Faust, ET Sakalli, CC Westrick, G Hussey, IP Conner, VLN Fu, SF Badylak, MB Steketee
    Sci Rep, 2019;9(1):3482.
    Species: Rat
    Sample Types: Cell Culture Supernates
  29. Polyphenols-Rich Fruit (Euterpe edulis Mart.) Prevents Peripheral Inflammatory Pathway Activation by the Short-Term High-Fat Diet
    Authors: AB Santamarin, G Jamar, LV Mennitti, DA Ribeiro, CM Cardoso, VV de Rosso, LM Oyama, LP Pisani
    Molecules, 2019;24(9):.
    Species: Rat
    Sample Types: Tissue Homogenates
  30. Elevated pulmonary arterial pressure in Zucker diabetic fatty rats
    Authors: D Morales-Ca, M Callejo, B Barreira, G Mondejar-P, S Esquivel-R, S Ramos, MÁ Martín, A Cogolludo, L Moreno, F Perez-Vizc
    PLoS ONE, 2019;14(1):e0211281.
    Species: Rat
    Sample Types: Serum
  31. Hyperbilirubinemia in Gunn Rats is Associated with Decreased Inflammatory Response in LPS-Mediated Systemic Inflammation
    Authors: P Valaskova, A Dvorak, M Lenicek, K Zizalova, N Kutinova-C, J Zelenka, M Cahova, L Vitek, L Muchova
    Int J Mol Sci, 2019;20(9):.
    Species: Rat
    Sample Types: Serum
  32. The Preventive Effects of Greenshell Mussel (Perna canaliculus) on Early-Stage Metabolic Osteoarthritis in Rats with Diet-Induced Obesity
    Authors: P Siriarchav, MC Kruger, MR Miller, HS Tian, FM Wolber
    Nutrients, 2019;11(7):.
    Species: Rat
    Sample Types: Plasma
  33. A diet including xanthan gum triggers a pro-inflammatory response in Wistar rats inoculated with Walker 256 cells
    Authors: AB Silva Risc, NIP Neto, K Gascho, M Carnier, DA de Miranda, FP Silva, VT Boldarine, M Seelaender, EB Ribeiro, LM Oyama, CM Oller do N
    PLoS ONE, 2019;14(6):e0218567.
    Species: Rat
    Sample Types: Tissue Homogenates
  34. Improvement of diabetic wound healing by topical application of Vicenin-2 hydrocolloid film on Sprague Dawley rats
    Authors: WS Tan, P Arulselvan, SF Ng, CN Mat Taib, MN Sarian, S Fakurazi
    BMC Complement Altern Med, 2019;19(1):20.
    Species: Rat
    Sample Types: Tissue Homogenates
  35. Early exposure to distinct sources of lipids affects differently the development and hepatic inflammatory profiles of 21-day-old rat offspring
    Authors: LV Mennitti, LM Oyama, AB Santamarin, CMDPO do Nascime, LP Pisani
    J Inflamm Res, 2018;11(0):11-24.
    Species: Rat
    Sample Types: Tissue Homogenates
  36. Lactobacillus fermentum HP3-Mediated Fermented Hericium erinaceus Juice as a Health Promoting Food Supplement to Manage Diabetes Mellitus
    Authors: C Chaiyasut, S Woraharn, BS Sivamaruth, N Lailerd, P Kesika, S Peerajan
    J Evid Based Integr Med, 2018;23(0):2515690X18765.
    Species: Rat
    Sample Types: Serum
  37. Cortical stimulation in conscious rats controls joint inflammation
    Authors: GS Bassi, L Ulloa, VR Santos, F Del Vecchi, P Delfino-Pe, GJ Rodrigues, JA Castania, FQ Cunha, HC Salgado, TM Cunha, N Garcia-Cai, A Kanashiro
    Prog. Neuropsychopharmacol. Biol. Psychiatry, 2018;0(0):.
    Species: Rat
    Sample Types: Synovial Fluid
  38. Characterization of the Myocardial Inflammatory Response in Acute Stress-Induced (Takotsubo) Cardiomyopathy
    Authors: HM Wilson, L Cheyne, PAJ Brown, K Kerr, A Hannah, J Srinivasan, N Duniak, G Horgan, DK Dawson
    JACC Basic Transl Sci, 2018;3(6):766-778.
    Species: Rat
    Sample Types: Serum
  39. Activation of trigeminal ganglion satellite glial cells in CFA-induced tooth pulp pain in rats
    Authors: HF Filippini, PA Scalzilli, KM Costa, RDS Freitas, MM Campos
    PLoS ONE, 2018;13(11):e0207411.
    Species: Rat
    Sample Types: Serum
  40. Obesity and Type 2 Diabetes mellitus induce lipopolysaccharide tolerance in rat neutrophils
    Authors: WMT Kuwabara, CNF Yokota, R Curi, TC Alba-Loure
    Sci Rep, 2018;8(1):17534.
    Species: Rat
    Sample Types: BALF
  41. The Use of Ju�ara (Euterpe edulis Mart.) Supplementation for Suppression of NF-?B Pathway in the Hypothalamus after High-Fat Diet in Wistar Rats
    Authors: AB Santamarin, G Jamar, LV Mennitti, VV de Rosso, HC Cesar, LM Oyama, LP Pisani
    Molecules, 2018;23(7):.
    Species: Rat
    Sample Types: Tissue Homogenates
  42. Leukemia inhibitory factor modulates the peripheral immune response in a rat model of emergent large vessel occlusion
    Authors: SM Davis, LA Collier, ED Winford, CC Leonardo, CT Ajmo, EA Foran, TJ Kopper, JC Gensel, KR Pennypacke
    J Neuroinflammation, 2018;15(1):288.
    Species: Rat
    Sample Types: Tissue Homogenates
  43. Effect of different intensities of physical activity on cardiometabolic markers and vascular and cardiac function in adult rats fed with a high-fat high-carbohydrate diet
    Authors: RB Batacan, MJ Duncan, VJ Dalbo, GL Buitrago, AS Fenning
    J Sport Health Sci, 2018;7(1):109-119.
    Species: Rat
    Sample Types: Serum
  44. Human Mesenchymal Stem Cell Secretome from Bone Marrow or Adipose-Derived Tissue Sources for Treatment of Hypoxia-Induced Pulmonary Epithelial Injury
    Authors: N Shologu, M Scully, JG Laffey, D O'Toole
    Int J Mol Sci, 2018;19(10):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  45. The plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo
    Authors: SU Vay, LJ Flitsch, M Rabenstein, R Rogall, S Blaschke, J Kleinhaus, N Reinert, A Bach, GR Fink, M Schroeter, MA Rueger
    J Neuroinflammation, 2018;15(1):226.
    Species: Rat
    Sample Types: Cell Culture Supernates
  46. Different Dietary Proportions of Fish Oil Regulate Inflammatory Factors but Do Not Change Intestinal Tight Junction ZO-1 Expression in Ethanol-Fed Rats
    Authors: YW Chien, HC Peng, YL Chen, MH Pai, HY Wang, HL Chuang, SC Yang
    Mediators Inflamm., 2017;2017(0):5801768.
    Species: Rat
    Sample Types: Tissue Homogenates
  47. Pyrrolidine dithiocarbamate administered during ex-vivo lung perfusion promotes rehabilitation of injured donor rat lungs obtained after prolonged warm ischemia
    Authors: C Francioli, X Wang, R Parapanov, E Abdelnour, J Lugrin, F Gronchi, J Perentes, P Eckert, HB Ris, L Piquilloud, T Krueger, L Liaudet
    PLoS ONE, 2017;12(3):e0173916.
    Species: Rat
    Sample Types: BALF
  48. The protective role of nitric oxide-dependent innate immunosuppression in the early stage of cartilage damage in rats: Role of nitric oxide in cartilage damage
    Authors: CC Hsu, CL Lin, IM Jou, PH Wang, JS Lee
    Bone Joint Res, 2017;6(4):253-258.
    Species: Rat
    Sample Types: Tissue Homogenates
  49. ?9-Tetrahydrocannabinol Prevents Cardiovascular Dysfunction in STZ-Diabetic Wistar-Kyoto Rats
    Authors: RK Vella, DJ Jackson, AS Fenning
    Biomed Res Int, 2017;2017(0):7974149.
    Species: Rat
    Sample Types: Serum
  50. Carotid sinus nerve electrical stimulation in conscious rats attenuates systemic inflammation via chemoreceptor activation
    Authors: FM Santos-Alm, G Domingos-S, CA Meschiari, LC Fávaro, C Becari, JA Castania, A Lopes, TM Cunha, DJA Moraes, FQ Cunha, L Ulloa, A Kanashiro, GCSV Tezini, HC Salgado
    Sci Rep, 2017;7(1):6265.
    Species: Rat
    Sample Types: Plasma
  51. Food-grade TiO2 impairs intestinal and systemic immune homeostasis, initiates preneoplastic lesions and promotes aberrant crypt development in the rat colon
    Authors: S Bettini, E Boutet-Rob, C Cartier, C Com‚ra, E Gaultier, J Dupuy, N Naud, S Tach‚, P Grysan, S Reguer, N Thieriet, M R‚fr‚giers, D ThiaudiŠre, JP Cravedi, M CarriŠre, JN Audinot, FH Pierre, L Guzylack-P, E Houdeau
    Sci Rep, 2017;7(0):40373.
    Species: Rat
    Sample Types: Cell Culture Supernates
  52. Incline treadmill exercise suppresses pain hypersensitivity associated with the modulation of pro-inflammatory cytokines and anti-inflammatory cytokine in rats with peripheral nerve injury
    Authors: KL Tsai, PC Huang, LK Wang, CH Hung, YW Chen
    Neurosci. Lett, 2017;643(0):27-31.
    Species: Rat
    Sample Types: Tissue Homogenates
  53. Cholinergic Stimulation Improves Oxidative Stress and Inflammation in Experimental Myocardial Infarction
    Authors: OC Bezerra, CM França, JA Rocha, GA Neves, PRM Souza, M Teixeira G, C Malfitano, TCA Loleiro, PM Dourado, S Llesuy, K de Angelis, MCC Irigoyen, L Ulloa, FM Consolim-C
    Sci Rep, 2017;7(1):13687.
    Species: Rat
    Sample Types: Tissue Homogenates
  54. Intestinal anti-inflammatory activity of Ground Cherry (Physalis angulata L.) standardized CO2 phytopharmaceutical preparation
    Authors: LD Almeida Ju, AEV Quaglio, CAR de Almeida, LC Di Stasi
    World J. Gastroenterol., 2017;23(24):4369-4380.
    Species: Rat
    Sample Types: Tissue Homogenates
  55. Impact of gestational nicotine exposure on intrauterine and fetal infection in a rodent model
    Authors: M von Chamie, L Reyes, LF Hayward, MB Brown
    Biol. Reprod., 2017;96(5):1071-1084.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: ELISA Capture
  56. Impact of exercise training associated to pyridostigmine treatment on autonomic function and inflammatory profile after myocardial infarction in rats
    Authors: Bruno Rodrigues
    Int. J. Cardiol., 2016;0(0):.
    Species: Rat
    Sample Types: Plasma
  57. Enteral sesame oil therapeutically relieves disease severity in rat experimental osteoarthritis
    Authors: DZ Hsu, PY Chu, IM Jou
    Food Nutr Res, 2016;60(0):29807.
    Species: Rat
    Sample Types: Tissue Homogenates
  58. Intra-articular blockade of P2X7 receptor reduces the articular hyperalgesia and inflammation in the knee joint synovitis especially in female rats
    Authors: Cláudia Herrera Tambeli
    J Pain, 2016;0(0):.
    Species: Rat
    Sample Types: Synovial Lavage Fluid
  59. Biodistribution and Clearance of TiO2 Nanoparticles in Rats after Intravenous Injection.
    Authors: Elgrabli D, Beaudouin R, Jbilou N, Floriani M, Pery A, Rogerieux F, Lacroix G
    PLoS ONE, 2015;10(4):e0124490.
    Species: Rat
    Sample Types: Serum
  60. Differential activation of inflammatory pathways in testicular macrophages provides a rationale for their subdued inflammatory capacity.
    Authors: Bhushan S, Tchatalbachev S, Lu Y, Frohlich S, Fijak M, Vijayan V, Chakraborty T, Meinhardt A
    J Immunol, 2015;194(11):5455-64.
    Species: Rat
    Sample Types: Cell Culture Supernates
  61. Reduced inflammatory phenotype in microglia derived from neonatal rat spinal cord versus brain.
    Authors: Baskar Jesudasan, Sam Josh, Todd, Kathryn, Winship, Ian R
    PLoS ONE, 2014;9(6):e99443.
    Species: Rat
    Sample Types: Cell Culture Supernates
  62. S1P1 receptor modulation preserves vascular function in mesenteric and coronary arteries after CPB in the rat independent of depletion of lymphocytes.
    Authors: Samarska I, Bouma H, Buikema H, Mungroop H, Houwertjes M, Absalom A, Epema A, Henning R
    PLoS ONE, 2014;9(5):e97196.
    Species: Rat
    Sample Types: Plasma
  63. Oligofructose supplementation (10%) during pregnancy and lactation does not change the inflammatory effect of concurrent trans fatty acid ingestion on 21-day-old offspring.
    Authors: Hachul A, Mennitti L, de Oliveira J, Moreno M, Okuda M, Dos Santos B, Oyama L, Ribeiro E, do Nascimento C, Pisani L
    Lipids Health Dis, 2013;12(0):59.
  64. Action at a distance: systemically administered adult stem/progenitor cells (MSCs) reduce inflammatory damage to the cornea without engraftment and primarily by secretion of TNF-alpha stimulated gene/protein 6.
    Authors: Roddy GW, Oh JY, Lee RH, Bartosh TJ, Ylostalo J, Coble K, Rosa RH, Prockop DJ
    Stem Cells, 2011;29(10):1572-9.
    Species: Rat
    Sample Types: Tissue Homogenates
  65. TNF-alpha, IL-1beta, IL-6, and cinc-1 levels in rat brain after meningitis induced by Streptococcus pneumoniae.
    Authors: Barichello T, dos Santos I, Savi GD, Simoes LR, Silvestre T, Comim CM, Sachs D, Teixeira MM, Teixeira AL, Quevedo J
    J. Neuroimmunol., 2010;221(1):42-5.
    Species: Rat
    Sample Types: Tissue Homogenates
  66. Supernatant of stored platelets causes lung inflammation and coagulopathy in a novel in vivo transfusion model.
    Authors: Vlaar AP, Hofstra JJ, Kulik W
    Blood, 2010;116(8):1360-8.
    Species: Rat
    Sample Types: Tissue Homogenates
  67. Depot-specific modulation of adipokine levels in rat adipose tissue by diet-induced obesity: the effect of aerobic training and energy restriction.
    Authors: Yamashita AS, Lira FS, Rosa JC, Paulino EC, Brum PC, Negrao CE, dos Santos RV, Batista ML, do Nascimento CO, Oyama LM, Seelaender M
    Cytokine, 2010;52(3):168-74.
    Species: Rat
    Sample Types: Tissue Homogenates
  68. Interleukin-1 mediates the anorexic and febrile actions of galanin-like Peptide.
    Authors: Man PS, Lawrence CB
    Endocrinology, 2008;149(11):5791-802.
    Species: Rat
    Sample Types: Plasma
  69. Comparative studies on the interplay of testosterone, estrogen and progesterone in collagen induced arthritis in rats.
    Authors: Ganesan K, Balachandran C, Manohar BM, Puvanakrishnan R
    Bone, 2008;43(4):758-65.
    Species: Rat
    Sample Types: Serum
  70. Augmentation of endogenous cannabinoid tone modulates lipopolysaccharide-induced alterations in circulating cytokine levels in rats.
    Authors: Roche M, Kelly JP, O'Driscoll M, O'Driscoll M, Finn DP
    Immunology, 2008;125(2):263-71.
    Species: Rat
    Sample Types: Plasma
  71. Haemostatic imbalance following carrageenan-induced rat paw oedema.
    Authors: Cicala C, Morello S, Alfieri A, Vellecco V, Marzocco S, Autore G
    Eur. J. Pharmacol., 2007;577(1):156-61.
    Species: Rat
    Sample Types: Serum
  72. MAPKs are key players in mediating cytokine release and cell death induced by unconjugated bilirubin in cultured rat cortical astrocytes.
    Authors: Fernandes A, Falcao AS, Silva RF, Brito MA, Brites D
    Eur. J. Neurosci., 2007;25(4):1058-68.
    Species: Rat
    Sample Types: Cell Culture Supernates
  73. Sirtuin regulates cigarette smoke-induced proinflammatory mediator release via RelA/p65 NF-kappaB in macrophages in vitro and in rat lungs in vivo: implications for chronic inflammation and aging.
    Authors: Yang SR, Wright J, Bauter M, Seweryniak K, Kode A, Rahman I
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;292(2):L567-76.
    Species: Rat
    Sample Types: BALF
  74. Cardiomyocyte function after burn injury and lipopolysaccharide exposure: single-cell contraction analysis and cytokine secretion profile.
    Authors: Niederbichler AD, Westfall MV, Su GL, Donnerberg J, Usman A, Vogt PM, Ipaktchi KR, Arbabi S, Wang SC, Hemmila MR
    Shock, 2006;25(2):176-83.
    Species: Rat
    Sample Types: Cell Culture Supernates
  75. Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis.
    Authors: Haas CS, Amin MA, Allen BB, Ruth JH, Haines GK, Woods JM, Koch AE
    Arthritis Rheum., 2006;54(8):2402-14.
    Species: Rat
    Sample Types: Tissue Homogenates
  76. The influence of kainic acid on core temperature and cytokine levels in the brain.
    Authors: Oprica M, Spulber SD, Aronsson AF, Post C, Winblad B, Schultzberg M
    Cytokine, 2006;35(1):77-87.
    Species: Rat
    Sample Types: Tissue Homogenates
  77. Role of matrix metalloproteinases in the inflammatory response in human airway cell-based assays and in rodent models of airway disease.
    Authors: Birrell MA, Wong S, Dekkak A, De Alba J, Haj-Yahia S, Belvisi MG
    J. Pharmacol. Exp. Ther., 2006;318(2):741-50.
    Species: Rat
    Sample Types: Tissue Homogenates
  78. Inflammatory signalling pathways involved in astroglial activation by unconjugated bilirubin.
    Authors: Fernandes A, Falcao AS, Silva RF, Gordo AC, Gama MJ, Brito MA, Brites D
    J. Neurochem., 2006;96(6):1667-79.
    Species: Rat
    Sample Types: Cell Culture Supernates

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Rat IL-6 DuoSet ELISA
By Anonymous on 08/11/2020
Application: Sample Tested: broncho-alveloar lavage fluid,Serum,Tissue Homogenates

Rat IL-6 DuoSet ELISA
By Anonymous on 08/21/2016
Application: Sample Tested: Cell Culture Supernates,Plasma