Rat IL-7 R alpha /CD127 PE-conjugated Antibody

    
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  • Species Reactivity
    Rat
  • Specificity
    Detects rat IL-7 R alpha /CD127 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2A Clone # 717519
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant rat IL-7 R alpha /CD127
    Glu21-Asp239
    Accession # NP_001099888
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of IL‑7 R alpha /CD127 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Anti-Rat CD4 APC-conjugated Antibody and either (A) Mouse Anti-Rat IL‑7 R alpha /CD127 PE‑conjugated Monoclonal Antibody (Catalog # FAB8484P) or (B) Mouse IgG2A Phycoerythrin Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-7 R alpha/CD127

IL-7 R alpha (IL-7 Receptor alpha), also known as CD127, is a 60-70 kDa member of the type I cytokine receptor family of molecules. It is expressed on resting naïve and memory CD8+ T cells, DN thymocytes, pre-B cells, Th1 CD4+ T cells, dendritic cells and monocytes. IL-7 R alpha heterodimerizes with both the gamma c chain to create the IL-7 receptor, and the TSLPR subunit to generate the TSLP Receptor. IL-7 R alpha participates in T cell differentiation, naïve T cell survival, and CD4+ thymocyte proliferation. Mature rat IL-7 R alpha is a 437 amino acid (aa) type I transmembrane glycoprotein. It contains a 219 aa extracellular region (aa 21-239) that possesses one type III Fibronectin domain (aa 129-222), and a 193 aa cytoplasmic tail that shows a key phosphorylation site at Tyr447. Over aa 21-239, rat IL-7 R alpha shares 79% and 67% aa identity with mouse and human IL-7 R alpha, respectively.

  • Long Name:
    Interleukin 7 Receptor alpha
  • Entrez Gene IDs:
    3575 (Human); 16197 (Mouse); 294797 (Rat)
  • Alternate Names:
    CD127 antigen; CD127; CD127ILRA; CDW127; IL-7 R alpha; IL-7 receptor subunit alpha; IL7R alpha; IL-7R subunit alpha; IL7R; IL7RA; IL-7Ra; IL-7R-alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; interleukin 7 receptor; interleukin-7 receptor subunit alpha
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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A PE-conjugated Antibody

Ctrl IC003P 14  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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