|Detection of IL‑7 R alpha /CD127 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Anti-Rat CD4 APC-conjugated Antibody and either (A) Mouse Anti-Rat IL‑7 R alpha /CD127 PE‑conjugated Monoclonal Antibody (Catalog # FAB8484P) or (B) Mouse IgG2A Phycoerythrin Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.|
IL-7 R alpha (IL-7 Receptor alpha), also known as CD127, is a 60-70 kDa member of the type I cytokine receptor family of molecules. It is expressed on resting naïve and memory CD8+ T cells, DN thymocytes, pre-B cells, Th1 CD4+ T cells, dendritic cells and monocytes. IL-7 R alpha heterodimerizes with both the gamma c chain to create the IL-7 receptor, and the TSLPR subunit to generate the TSLP Receptor. IL-7 R alpha participates in T cell differentiation, naïve T cell survival, and CD4+ thymocyte proliferation. Mature rat IL-7 R alpha is a 437 amino acid (aa) type I transmembrane glycoprotein. It contains a 219 aa extracellular region (aa 21-239) that possesses one type III Fibronectin domain (aa 129-222), and a 193 aa cytoplasmic tail that shows a key phosphorylation site at Tyr447. Over aa 21-239, rat IL-7 R alpha shares 79% and 67% aa identity with mouse and human IL-7 R alpha, respectively.