Rat IL-7R alpha/CD127 Antibody

Catalog # Availability Size / Price Qty
MAB8484-100
MAB8484-SP
Detection of IL‑7 R alpha /CD127 in CD4+Rat Splenocytes by Flow Cytometry.
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Rat IL-7R alpha/CD127 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat IL‑7 R alpha /CD127in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 717519
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat IL-7 R alpha /CD127
Glu21-Asp239
Accession # NP_001099888
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of IL-7 R alpha /CD127 antibody in CD4<sup>+</sup>Rat Splenocytes antibody by Flow Cytometry. View Larger

Detection of IL‑7 R alpha /CD127 in CD4+Rat Splenocytes by Flow Cytometry. CD4+rat splenocytes were stained with Mouse Anti-Rat IL-7 R alpha /CD127 Monoclonal Antibody (Catalog # MAB8484, filled histogram) or Mouse IgG2A Isotype Control (Catalog # MAB003, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-7R alpha/CD127

IL-7 R alpha (IL-7 receptor alpha; also CD127) is a 60-70 kDa member of the type I cytokine receptor family of molecules. It is expressed on resting naïve and memory CD8+ T cells, DN thymocytes, pre-B cells, Th1 CD4+ T cells, dendritic cells and monocytes. IL-7 R alpha heterodimerizes with both the gamma c chain to create the IL-7 receptor, and the TSLPR subunit to generate the TSLP receptor. IL-7 R alpha participates in T cell differentiation, naïve T cell survival, and CD4+ thymocyte proliferation. Mature rat IL-7 R alpha is a 437 aa type I transmembrane glycoprotein. It contains a 219 aa extracellular region (aa 21-239) that possesses one type III fibronectin domain (aa 129‑222), and a 193 aa cytoplasmic tail that shows a key phosphorylation site at Tyr447. Over aa 21‑239, rat IL-7 R alpha shares 79% and 67% aa identity with mouse and human IL-7 R alpha, respectively.

Long Name
Interleukin 7 Receptor alpha
Entrez Gene IDs
3575 (Human); 16197 (Mouse); 294797 (Rat); 102143679 (Cynomolgus Monkey)
Alternate Names
CD127 antigen; CD127; CD127ILRA; CDW127; IL-7 R alpha; IL-7 receptor subunit alpha; IL7R alpha; IL-7R alpha; IL-7R subunit alpha; IL7R; IL7RA; IL-7Ra; IL-7R-alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; interleukin 7 receptor; interleukin-7 receptor subunit alpha

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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