Rat LMAN1L/SLAMP Antibody

Catalog # Availability Size / Price Qty
AF6017
AF6017-SP
Detection of Rat LMAN1L/SLAMP by Western Blot.
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Rat LMAN1L/SLAMP Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat LMAN1L/SLAMP in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant rat LMAN1L/SLAMP
Arg33-Thr436
Accession # Q5FB95
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Rat LMAN1L/SLAMP antibody by Western Blot. View Larger

Detection of Rat LMAN1L/SLAMP by Western Blot. Western blot shows lysates of rat salivary gland tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Rat LMAN1L/SLAMP Polyclonal Antibody (Catalog # AF6017) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LMAN1L/SLAMP at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 02.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LMAN1L/SLAMP

SLAMP (Sublingual acinar membrane protein; also LMAN1L) is a 50‑60 kDa protein that likely is a member of the animal L‑type lectin family of proteins. It is expressed exclusively by mucous secreting cells in the ER‑Golgi compartment. SLAMP likely plays a role in the intracellular trafficking of glycoproteins, possibly by binding mannose residues. Mature rat SLAMP is a 475 amino acid (aa) type I transmembrane protein that contains a 409 aa extracellular region (aa 29‑437) and a 43 aa cytoplasmic domain (aa 461‑503). Over aa 33‑436, rat SLAMP shares 89% aa identity with mouse SLAMP.

Long Name
Lectin Mannose-binding 1 Like/Sublingual Acinar Membrane Protein
Entrez Gene IDs
235416 (Mouse); 300743 (Rat)
Alternate Names
ERGL; lectin, mannose-binding, 1 like; LMAN1L; SLAMP

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