Rat LMAN1L/SLAMP Antibody

  
  • Species Reactivity
    Rat
  • Specificity
    Detects rat LMAN1L/SLAMP in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant rat LMAN1L/SLAMP
    Arg33-Thr436
    Accession # Q5FB95
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rat LMAN1L/SLAMP by Western Blot. Western blot shows lysates of rat salivary gland tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Rat LMAN1L/SLAMP Polyclonal Antibody (Catalog # AF6017) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LMAN1L/SLAMP at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 02.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LMAN1L/SLAMP
SLAMP (Sublingual acinar membrane protein; also LMAN1L) is a 50‑60 kDa protein that likely is a member of the animal L‑type lectin family of proteins. It is expressed exclusively by mucous secreting cells in the ER‑Golgi compartment. SLAMP likely plays a role in the intracellular trafficking of glycoproteins, possibly by binding mannose residues. Mature rat SLAMP is a 475 amino acid (aa) type I transmembrane protein that contains a 409 aa extracellular region (aa 29‑437) and a 43 aa cytoplasmic domain (aa 461‑503). Over aa 33‑436, rat SLAMP shares 89% aa identity with mouse SLAMP.
  • Long Name:
    Lectin Mannose-binding 1 Like/Sublingual Acinar Membrane Protein
  • Entrez Gene IDs:
    235416 (Mouse); 300743 (Rat)
  • Alternate Names:
    ERGL; lectin, mannose-binding, 1 like; LMAN1L; SLAMP
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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