Rat LMAN1L/SLAMP Antibody

Catalog #: AF6017 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6017
AF6017-SP

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Detection of Rat LMAN1L/SLAMP by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat LMAN1L/SLAMP Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat LMAN1L/SLAMP in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant rat LMAN1L/SLAMP
Arg33-Thr436
Accession # Q5FB95

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Rat LMAN1L/SLAMP by Western Blot. Western blot shows lysates of rat salivary gland tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Rat LMAN1L/SLAMP Polyclonal Antibody (Catalog # AF6017) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LMAN1L/SLAMP at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 02.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LMAN1L/SLAMP

SLAMP (Sublingual acinar membrane protein; also LMAN1L) is a 50‑60 kDa protein that likely is a member of the animal L‑type lectin family of proteins. It is expressed exclusively by mucous secreting cells in the ER‑Golgi compartment. SLAMP likely plays a role in the intracellular trafficking of glycoproteins, possibly by binding mannose residues. Mature rat SLAMP is a 475 amino acid (aa) type I transmembrane protein that contains a 409 aa extracellular region (aa 29‑437) and a 43 aa cytoplasmic domain (aa 461‑503). Over aa 33‑436, rat SLAMP shares 89% aa identity with mouse SLAMP.

Long Name

Lectin Mannose-binding 1 Like/Sublingual Acinar Membrane Protein

Entrez Gene IDs

235416 (Mouse); 300743 (Rat)

Alternate Names

ERGL; lectin, mannose-binding, 1 like; LMAN1L; SLAMP