Rat Luminex® Discovery Assay

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Rat Magnetic Luminex Assay
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Product Details
Citations (23)
Supplemental Products
Reviews (2)

Rat Luminex® Discovery Assay Summary

Assay Type Magnetic bead-based multiplex assay for the Luminex® platform
Format 1 x 96-well microplate and Magnetic antibody-coated beads
Species Rat
Analytes Detected Please see analyte list in assay customization tool below.
Performance Validation Luminex Assays are validated for use with cell culture supernatants, plasma and serum. All Luminex assays are tested for sensitivity, intra-assay precision, inter-assay precision and to ensure assay linearity for validated sample types. Antibody pairs are selected and tested to confirm the parallel detection of natural and recombinant standard protein and to ensure the accurate determination of target analytes within biological samples. Assays for each target analyte are screened against all target analytes to confirm low antibody cross-reactivity.

Make your selections below to configure your assay

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Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.

If you run into bead region incompatibilities, send your Luminex code to our team and find out more.

How would you like these analytes mixed?

User Mixed
Please select at least one mixing option to see the kits.

Would you like to split based on dilution?

Do not split
Split based on serum
Split based on plasma
Split based on cell culture supernates

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.

Product Features

Magnetic Luminex Assays are the most flexible bead-based multiplex assays that we offer. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples.

  • Simultaneously profile up to 50 analytes of your choice in one sample
  • Choose from a wide menu of analytes, including many unique analytes
  • Requires a small sample volume (<50 µL)
  • Run your assay in just 3-4 hours
  • Mass-calibrated standards for consistent results with every new lot of material
  • Enough reagents for one 96-well plate
  • Quick turn-around time: Kits are typically shipped within 5-10 days of ordering.*

Please allow additional time for international orders.

Kit Components
  • Premixed cocktail of antibody-coated Magnetic beads
  • Premixed cocktail of biotinylated detection antibody
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Standard/Sample Diluent
  • Wash Buffer
  • Streptavidin-PE
  • One flat-bottom 96-well Microplate
  • Foil Plate Sealers (4)
  • Mixing Bottle
  • Certificate of Analysis**

**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.

Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

Product Datasheets

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Product Specific Notices
Luminex® is a registered trademark of Luminex Corporation.

Citations for Rat Luminex® Discovery Assay

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Cannabinoid Receptor 2 Blockade Prevents Anti-Depressive-like Effect of Cannabidiol Acid Methyl Ester in Female WKY Rats
    Authors: D Hen-Shoval, L Moshe, T Indig-Naim, R Mechoulam, G Shoval, G Zalsman, NM Kogan, A Weller
    International Journal of Molecular Sciences, 2023-02-14;24(4):.
    Species: Rat
    Sample Types: Plasma
  2. Design, Optimization and Evaluation of Dexamethasone-Loaded Microneedles for Inflammatory Disorders
    Authors: S Duarah, M Sharma, S Chen, TK Proft, J Loh, J Wen
    International journal of pharmaceutics, 2023-02-04;0(0):122690.
    Species: Rat
    Sample Types: Plasma
  3. Local IL-10 delivery modulates the immune response and enhances repair of volumetric muscle loss muscle injury
    Authors: T Huynh, C Reed, Z Blackwell, P Phelps, LCP Herrera, J Almodovar, DA Zaharoff, J Wolchok
    Scientific Reports, 2023-02-03;13(1):1983.
    Species: Rat
    Sample Types: Tissue Homogenates
  4. Long-term anti-tumor effects following both conventional radiotherapy and FLASH in fully immunocompetent animals with glioblastoma
    Authors: E Liljedahl, E Konradsson, E Gustafsson, KF Jonsson, JK Olofsson, C Ceberg, HN Redebrandt
    Scientific Reports, 2022-07-19;12(1):12285.
    Species: Rat
    Sample Types: Serum
  5. Social Isolation Stress Modulates Pregnancy Outcomes and the Inflammatory Profile of Rat Uterus
    Authors: NA Lopes, EA Falkenberg, C Wiley, V Patel, J Serrano-Lo, X Fang, AM Weiler, JK McCreary, GAS Metz, DM Olson
    International Journal of Molecular Sciences, 2022-05-31;23(11):.
    Species: Rat
    Sample Types: Tissue Homogenates
  6. Intramuscular injection of skeletal muscle derived extracellular matrix mitigates denervation atrophy after sciatic nerve transection
    Authors: BK Schilling, JS Baker, C Komatsu, KG Marra
    Journal of tissue engineering, 2021-09-19;12(0):2041731421103.
    Species: Rat
    Sample Types: Tissue Homogenates
  7. Key indicators of repetitive overuse-induced neuromuscular inflammation and fibrosis are prevented by manual therapy in a rat model
    Authors: MF Barbe, MY Harris, GE Cruz, M Amin, NM Billett, JT Dorotan, EP Day, SY Kim, GM Bove
    Bmc Musculoskeletal Disorders, 2021-05-05;22(1):417.
    Species: Mouse
    Sample Types: Tissue Homogenates
  8. Evaluation of neurofilament light chain in the cerebrospinal fluid and blood as a biomarker for neuronal damage in experimental pneumococcal meningitis
    Authors: ND Le, L Muri, D Grandgirar, J Kuhle, D Leppert, SL Leib
    J Neuroinflammation, 2020-10-07;17(1):293.
    Species: Rat
    Sample Types: CSF
  9. Repetitive transcranial magnetic stimulation activates glial cells and inhibits neurogenesis after pneumococcal meningitis
    Authors: L Muri, S Oberhänsli, M Buri, ND Le, D Grandgirar, R Bruggmann, RM Müri, SL Leib
    PLoS ONE, 2020-09-11;15(9):e0232863.
    Species: Rat
    Sample Types: Cell Culture Supernates
  10. Anti-Inflammatory Effects of Magnetically Targeted Mesenchymal Stem Cells on Laser-Induced Skin Injuries in Rats
    Authors: X Li, Z Wei, W Zhang, H Lv, J Li, L Wu, H Zhang, B Yang, M Zhu, J Jiang
    Int J Nanomedicine, 2020-08-06;15(0):5645-5659.
    Species: Rat
    Sample Types: Cell Culture Supernates, Serum
  11. Ginseng-Induced Changes to Blood Vessel Dilation and the Metabolome of Rats
    Authors: HJ Lee, BM Kim, SH Lee, JT Sohn, JW Choi, CW Cho, HD Hong, YK Rhee, HJ Kim
    Nutrients, 2020-07-27;12(8):.
    Species: Rat
    Sample Types: Plasma
  12. Commiphora Extract Mixture Ameliorates Monosodium Iodoacetate-Induced Osteoarthritis
    Authors: D Lee, MK Ju, H Kim
    Nutrients, 2020-05-19;12(5):.
    Species: Rat
    Sample Types: Serum
  13. Baroreceptor denervation reduces inflammatory status but worsens cardiovascular collapse during systemic inflammation
    Authors: MR Amorim, JL de Deus, CA Pereira, LEV da Silva, GS Borges, NS Ferreira, ME Batalhão, J Antunes-Ro, EC Carnio, RC Tostes, LGS Branco
    Sci Rep, 2020-04-24;10(1):6990.
    Species: Rat
    Sample Types: Plasma
  14. Trauma induced tissue survival in vitro with a muscle-biomaterial based osteogenic organoid system: a proof of concept study
    Authors: T He, J Hausdorf, Y Chevalier, RM Klar
    BMC Biotechnol., 2020-01-31;20(1):8.
    Species: Rat
    Sample Types: Cell Culture Supernates
  15. Blunting neuroinflammation with resolvin D1 prevents early pathology in a rat model of Parkinson's disease
    Authors: P Krashia, A Cordella, A Nobili, L La Barbera, M Federici, A Leuti, F Campanelli, G Natale, G Marino, V Calabrese, F Vedele, V Ghiglieri, B Picconi, G Di Lazzaro, T Schirinzi, G Sancesario, N Casadei, O Riess, S Bernardini, A Pisani, P Calabresi, MT Viscomi, CN Serhan, V Chiurchiù, M D'Amelio, NB Mercuri
    Nat Commun, 2019-09-02;10(1):3945.
    Species: Rat
    Sample Types: Plasma
  16. Preconditioning of Rat Bone Marrow-Derived Mesenchymal Stromal Cells with Toll-Like Receptor Agonists
    Authors: F Evaristo-M, G Sardella-S, TH Kasai-Brun, RMP Campos, P Domizi, MF Santiago, RA de Melo Re, R Mendez-Ote, VT Ribeiro-Re, PM Pimentel-C
    Stem Cells Int, 2019-08-19;2019(0):7692973.
    Species: Rat
    Sample Types: Cell Culture Supernates
  17. Metformin mediates neuroprotection and attenuates hearing loss in experimental pneumococcal meningitis
    Authors: L Muri, ND Le, J Zemp, D Grandgirar, SL Leib
    J Neuroinflammation, 2019-07-27;16(1):156.
    Species: Rat
    Sample Types: CSF
  18. Neuroinflammation in the NTS is associated with changes in cardiovascular reflexes during systemic inflammation
    Authors: MR Amorim, JL de Deus, RA Cazuza, CMD Mota, LEV da Silva, GS Borges, ME Batalhão, EC Cárnio, LGS Branco
    J Neuroinflammation, 2019-06-20;16(1):125.
    Species: Rat
    Sample Types: Tissue Homogenates
  19. Thrombin Preconditioning of Extracellular Vesicles Derived from Mesenchymal Stem Cells Accelerates Cutaneous Wound Healing by Boosting Their Biogenesis and Enriching Cargo Content
    Authors: DK Sung, YS Chang, SI Sung, SY Ahn, WS Park
    J Clin Med, 2019-04-18;8(4):.
    Species: Rat
    Sample Types:
  20. Cardiovascular toxicity of decabrominated diphenyl ethers (BDE-209) and decabromodiphenyl ethane (DBDPE) in rats
    Authors: L Jing, Y Sun, Y Wang, B Liang, T Chen, D Zheng, X Zhao, X Zhou, Z Sun, Z Shi
    Chemosphere, 2019-02-18;223(0):675-685.
    Species: Rat
    Sample Types: Serum
  21. Comparative effects of dietary n-3 docosapentaenoic acid (DPA), DHA and EPA on plasma lipid parameters, oxidative status and fatty acid tissue composition
    Authors: G Drouin, D Catheline, E Guillochea, P Gueret, C Baudry, P Le Ruyet, V Rioux, P Legrand
    J. Nutr. Biochem., 2018-10-12;63(0):186-196.
    Species: Rat
    Sample Types: Plasma
  22. Mycolactone displays anti-inflammatory effects on the nervous system
    Authors: C Isaac, A Mauborgne, A Grimaldi, K Ade, M Pohl, C Limatola, Y Boucher, C Demangel, L Guenin-Mac
    PLoS Negl Trop Dis, 2017-11-17;11(11):e0006058.
    Species: Rat
    Sample Types: Tissue Homogenates
  23. Manual therapy as an effective treatment for fibrosis in a rat model of upper extremity overuse injury
    Authors: GM Bove, MY Harris, H Zhao, MF Barbe
    J. Neurol. Sci, 2015-12-24;361(0):168-80.
    Species: Rat
    Sample Types: Tissue Homogenates


  1. What is the reason for the recommendation to use 35 µL sample volume, instead of the default 50 µL volume, for Luminex multiplex containing > 25 analytes, when assayed on Luminex MAGPIX® analyzer?

    • R&D Systems recommend 35 µL sample volume for > 25 analytes to control the number of beads in the sample load when using Luminex MAGPIX® analyzer. Luminex MAGPIX® analyzer is a non-flow-based reader which utilizes a magnet to hold the magnetic microparticles in a monolayer in a chamber where the beads are imaged and then processed by the software to generate data. Since MAGPIX® system pushes the entire Sample Load Volume into the chamber, multiplex assay containing > 25 analytes may overload the chamber capacity with 50 µL sample load default setting. The captured images can have too many beads in them to give accurate results, and some of the analytes/regions may present lower than desired bead counts. 

  2. Does the Luminex Rat Magnetic Assay detect soluble form or membrane-bound analyte?

    • The sample types typically validated by R&D Systems for Luminex assays contain soluble form of proteins, and therefore it is expected that the  soluble form of proteins are detected. It is possible there are assays that also detect the membrane form of  respective target proteins, however, R&D Systems does not generally evaluate our assays against samples known to contain membrane-bound proteins.

  3. Is the reconstituted standard a concentrate?

    • For Discovery Assays, each standard cocktail is a concentrate upon reconstitution with the volume specified on the Certificate of Analysis (CoA). After creating Standard 1 following the table in the product insert, the Standard 1 value is the concentration listed on the CoA.

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By Flavia Millesi on 08/02/2023
Sample Tested: Olfactory enshathing cells,Schwann cells

By Anonymous on 11/20/2021
Sample Tested: Recombinant protein

in vitro work with rat recombinant protein