RbAp46 Antibody (2A10C2) - BSA Free

Novus Biologicals | Catalog # NBP3-27151

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2A10C2

Format

BSA Free
View all RbAp46 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human RbAp46 (AA: 1-200) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

47.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RbAp46 Antibody (2A10C2) - BSA Free

Immunohistochemistry: RbAp46 Antibody (2A10C2) [NBP3-27151] -

Immunohistochemistry: RbAp46 Antibody (2A10C2) [NBP3-27151] - Analysis of paraffin-embedded rectum cancer tissues using RbAp46 mouse mAb with DAB staining.

Western Blot: RbAp46 Antibody (2A10C2) [NBP3-27151] -

Western Blot: RbAp46 Antibody (2A10C2) [NBP3-27151] - Analysis using RbAp46 mAb against HEK293 (1) and RbAp46 (AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: RbAp46 Antibody (2A10C2) [NBP3-27151] -

ELISA: RbAp46 Antibody (2A10C2) [NBP3-27151] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Western Blot: RbAp46 Antibody (2A10C2) [NBP3-27151] -

Western Blot: RbAp46 Antibody (2A10C2) [NBP3-27151] - Analysis using RbAp46 mAb against human RbAp46 (AA: 1-200) recombinant protein. (Expected MW is 48.6 kDa)

Immunohistochemistry: RbAp46 Antibody (2A10C2) [NBP3-27151] -

Immunohistochemistry: RbAp46 Antibody (2A10C2) [NBP3-27151] - Analysis of paraffin-embedded bladder cancer tissues using RbAp46 mouse mAb with DAB staining.

Immunocytochemistry/Immunofluorescence: RbAp46 Antibody (2A10C2) [NBP3-27151] -

Immunocytochemistry/Immunofluorescence: RbAp46 Antibody (2A10C2) [NBP3-27151] - Analysis of Hela cells using RbAp46 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow Cytometry: RbAp46 Antibody (2A10C2) [NBP3-27151] -

Flow Cytometry: RbAp46 Antibody (2A10C2) [NBP3-27151] - Analysis of Hela cells using RbAp46 mouse mAb (green) and negative control (red).

Western Blot: RbAp46 Antibody (2A10C2) [NBP3-27151] -

Western Blot: RbAp46 Antibody (2A10C2) [NBP3-27151] - Analysis using RbAp46 mouse mAb against Jurkat (1), HepG2 (2), F9 (3), C6 (4), LNCAP (5), HL-60 (6), Hela (7), and SH-SY5Y (8) cell lysate.

Immunocytochemistry/Immunofluorescence: RbAp46 Antibody (2A10C2) [NBP3-27151] -

Immunocytochemistry/Immunofluorescence: RbAp46 Antibody (2A10C2) [NBP3-27151] - Analysis of Hela cells using RbAp46 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Applications for RbAp46 Antibody (2A10C2) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RbAp46

RBBP7 antibody, also known as RbAp46 (Retinoblastoma protein associated protein 46), is a nuclear protein that has been shown to interact directly with retinoblastoma protein and is a component of the mSin3 corepressor complex, as are the histone deacetylase proteins (HDACs). It shares about 75% sequence identity with the closely related RbAp48, and appears to be functionally related to the yeast MSI1 protein in that overexpression of RbAp46 suppresses heat shock sensitivity in a RAS pathway dependent manner. RBBP7 has also been demonstrated to be up-regulated by the Wilms' tumor suppressor gene product, WT1. In addition, cells transfected with this antibody have shown strong inhibition of growth.

Alternate Names

G1/S transition control protein-binding protein RbAp46, Histone acetyltransferase type B subunit 2, histone-binding protein RBBP7, MGC138867, Nucleosome-remodeling factor subunit RBAP46, RBAP46, RBBP-7, retinoblastoma binding protein 7, Retinoblastoma-binding protein 7MGC138868, Retinoblastoma-binding protein p46, retinoblastoma-binding protein RbAp46

Gene Symbol

RBBP7

Additional RbAp46 Products

Product Documents for RbAp46 Antibody (2A10C2) - BSA Free

Certificate of Analysis

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Product Specific Notices for RbAp46 Antibody (2A10C2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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