RBBP4/RbAp48 Antibody (11G10) - Azide and BSA Free

Novus Biologicals | Catalog # NB500-123

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Block/Neutralize, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Functional

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 11G10

Format

Azide and BSA Free
View all RBBP4 Primary Antibodies »

Product Specifications

Immunogen

The complete coding region (amino acids 1-425) of RBBP4/RbAp48 expressed in E. coli.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Please contact Technical Support if you have any questions.

Specificity

This is specific for full length RbAp48.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RBBP4/RbAp48 Antibody (11G10) - Azide and BSA Free

Western Blot: RBBP4/RbAp48 Antibody (11G10) [NB500-123]

Western Blot: RBBP4/RbAp48 Antibody (11G10) [NB500-123]

Western Blot: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [11G10] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (11G10) [NB500-123]

Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (11G10) [NB500-123]

Immunocytochemistry/Immunofluorescence: RbAp48 Antibody (1D11) [NB500-123] - Immunofluorescence analysis of HeLa, using RbAp48 antibody at 1:100 dilution.
Immunohistochemistry: RbAp48 Antibody (1D11) [NB500-123]
Chromatin Immunoprecipitation: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - HeLa whole cell lysate/extract A. 5 ug preimmune mouse IgG B. 5 ug of RbAp48 antibody. The precipitated DNA was detected by PCR with primer set targeting to SOX9 gene locus.
Western Blot: RBBP4/RbAp48 Antibody (11G10) [NB500-123]

Western Blot: RBBP4/RbAp48 Antibody (11G10) [NB500-123]

Western Blot: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [11G10] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunoprecipitation: RBBP4/RbAp48 Antibody (11G10) [NB500-123]

Immunoprecipitation: RBBP4/RbAp48 Antibody (11G10) [NB500-123]

Immunoprecipitation: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - Myc-tag antibody immunoprecipitation confirmed pulldown of NuRD component RbAp48 which is known to interact with ZNF827. * denotes IgG band at 55 kDa. Image submitted by a verified customer review.

Applications for RBBP4/RbAp48 Antibody (11G10) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500 - 1:1000

Immunohistochemistry

1:100 - 1:1000

Immunohistochemistry-Paraffin

1:100 - 1:1000

Western Blot

1:500 - 1:3000
Application Notes
ICC/IF: Permeabilization step recommended.

Reviewed Applications

Read 1 review rated 5 using NB500-123 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RBBP4

Clone 1D11 is a 48 kDa nuclear protein, first isolated by its ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). It is a member of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of three subunits of CAF-1, can bind to histone H4, and is a subunit of human histone deacetylase HD1. This particular hybridoma is a fusion between BALB/c splenocytes and mouse myeloma (NS-1 cells)

Long Name

Retinoblastoma Binding Protein 4

Alternate Names

CAF-1 p48, CAF-1C, NURF55, RBAP48

Entrez Gene IDs

5928 (Human)

Gene Symbol

RBBP4

Additional RBBP4 Products

Product Documents for RBBP4/RbAp48 Antibody (11G10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RBBP4/RbAp48 Antibody (11G10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RBBP4/RbAp48 Antibody (11G10) - Azide and BSA Free

Customer Reviews for RBBP4/RbAp48 Antibody (11G10) - Azide and BSA Free (1)

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  • RBBP4/RbAp48 Antibody (11G10)
    Name: Robert Lu
    Application: Immunoprecipitation
    Sample Tested: U-2 OS cells
    Species: Human
    Verified Customer | Posted 12/18/2020
    Myc-tag antibody immunoprecipitation confirmed pulldown of NuRD component RbAp48 which is known to interact with ZNF827. * denotes IgG band at 55kD
    ZNF827 was overexpressed in U-2 OS cells with a Myc-tag. Myc-tag IP was performed using CST #2276 antibody (Ms). Mouse IgG antibody was used as a control.
    RBBP4/RbAp48 Antibody (11G10) - Azide and BSA Free NB500-123

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