RBBP4/RbAp48 Antibody (2D7) - Azide and BSA Free

Novus Biologicals | Catalog # H00005928-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2D7

Format

Azide and BSA Free
View all RBBP4 Primary Antibodies »

Product Specifications

Immunogen

RBBP4 (AAH53904, 1 a.a. ~ 425 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MADKEAAFDDAVEERVINEEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLPDVTRPEGKDFSIHRLVLGTHTSDEQNHLVIASVQLPNDDAQFDASHYDSEKGEFGGFGSVSGKIEIEIKINHEGEVNRARYMPQNPCIIATKTPSSDVLVFDYTKHPSKPDPSGECNPDLRLRGHQKEGYGLSWNPNLSGHLLSASDDHTICLWDISAVPKEGKVVDAKTIFTGHTAVVEDVSWHLLHESLFGSVADDQKLMIWDTRSNNTSKPSHSVDAHTAEVNCLSFNPYSEFILATGSADKTVALWDLRNLKLKLHSFESHKDEIFQVQWSPHNETILASSGTDRRLNVWDLSKIGEEQSPEDAEDGPPELLFIHGGHTAKISDFSWNPNEPWVICSVSEDNIMQVWQMAENIYNDEDPEGSVDPEGQGS

Specificity

RBBP4 - retinoblastoma binding protein 4

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RBBP4/RbAp48 Antibody (2D7) - Azide and BSA Free

Western Blot: RBBP4/RbAp48 Antibody (2D7) [H00005928-M01]

Western Blot: RBBP4/RbAp48 Antibody (2D7) [H00005928-M01]

Western Blot: RBBP4/RbAp48 Antibody (2D7) [H00005928-M01] - RBBP4 monoclonal antibody (M01), clone 2D7 Analysis of RBBP4 expression in Hela.
Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (2D7) [H00005928-M01]

Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (2D7) [H00005928-M01]

Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (2D7) [H00005928-M01] - Analysis of monoclonal antibody to RBBP4 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (2D7) [H00005928-M01]

Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (2D7) [H00005928-M01]

Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (2D7) [H00005928-M01] - Analysis of monoclonal antibody to RBBP4 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. Antibody concentration 3 ug/ml.
ELISA: RBBP4/RbAp48 Antibody (2D7) [H00005928-M01]

ELISA: RBBP4/RbAp48 Antibody (2D7) [H00005928-M01]

ELISA: RBBP4/RbAp48 Antibody (2D7) [H00005928-M01] - Detection limit for recombinant GST tagged RBBP4 is approximately 0.3ng/ml as a capture antibody.

Applications for RBBP4/RbAp48 Antibody (2D7) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RBBP4

This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes.

Long Name

Retinoblastoma Binding Protein 4

Alternate Names

CAF-1 p48, CAF-1C, NURF55, RBAP48

Entrez Gene IDs

5928 (Human)

Gene Symbol

RBBP4

OMIM

602923 (Human)

UniProt

AAH53904 (Human)

Additional RBBP4 Products

Product Documents for RBBP4/RbAp48 Antibody (2D7) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for RBBP4/RbAp48 Antibody (2D7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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