Recombinant Cynomolgus IFN-alpha/beta R2 Fc Protein, CF

Catalog # Availability Size / Price Qty
10071-AB-050
Recombinant Cynomolgus IFN-alpha/beta R2 Fc Protein, CF Inhibition Activity
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Recombinant Cynomolgus IFN-alpha/beta R2 Fc Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Type-I IFN-mediated anti-viral activity. The ED50 for this effect is 0.5-3.5 μg/mL in the presence of 30 pg/mL of Recombinant Human IFN‑ beta (Catalog # 8499-IF).
Source
Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey IFN-alpha/beta R2 protein
Cynomolgus IFN-alpha R2
(Ile 27-Lys243)
Accession # P48551
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis

Ile 27

Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
51.4 kDa
SDS-PAGE
75 - 95 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10071-AB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Inhibition Activity Inhibition Activity View Larger

Recombinant Cynomolgus IFN-alpha/beta R2Fc Chimera (Catalog # 10071-AB) inhibits the anti-viral activity of (Catalog # 8499-IF) The ED50 for this effect is 0.5-3.5μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus Monkey IFN‑ alpha / beta R2 (Catalog # 10071-AB) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 75-95 kDa and 150-190 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-alpha/beta R2

IFN-alpha/beta R2, also known as IFNAR2, is a 100 kDa glycoprotein in the class II cytokine receptor family. These proteins form heterodimeric receptor complexes that transduce signals from the interferon, IL-10, and IL-28 families of cytokines (1, 2). Mature IFN-alpha / beta R2 consists of an extracellular domain (ECD), containing two fibronectin type III repeats, a transmembrane segment, and a cytoplasmic domain. Alternative splicing generates a secreted isoform that corresponds to the ECD as well as a 50 kDa transmembrane isoform with a substituted and truncated cytoplasmic region (3, 4). The short isoform is impaired in its ability to activate signaling molecules and functions as a dominant negative receptor subunit (5-7). The mature ECD of cynomolgus IFN-alpha/ beta R2 shares 93% and 100% amino acid (aa) sequence identity with that of human and rhesus, respectively. IFN-alpha/beta R2, in association with IFN-alpha/beta R1, is required for mediating the antiviral, antiproliferative, and apoptotic effects of the type I interferons IFN-alpha and IFN-beta. IFN-alpha / beta R2 is the principal ligand binding subunit of the receptor. Ligand binding is stabilized by the subsequent association with IFN-alpha / beta R1, resulting in the formation of a signaling ternary receptor complex (8, 9). IFN-alpha / beta R2 is also subject to presenilin-dependent intramembrane proteolysis, resulting in the liberation of nearly the entire ECD as well as the cytoplasmic domain which migrates to the nucleus and can inhibit gene transcription (10). High concentrations of soluble IFN-alpha / beta R2 bind and neutralize IFN-alpha and IFN-beta, while lower concentrations prolong the antiviral activity of circulating IFN-beta but not IFN-alpha (11). Human but not mouse IFN-alpha / beta R2 constitutively associates with STAT4, which may account for species specific differences observed in type I interferon responses (12).

References
  1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Lutfalla, G. et al. (1995) EMBO J. 14:5100.
  4. Novick, D. et al. (1995) J. Leukocyte Biol. 57:712.
  5. Pfeffer, L.M. et al. (1997) J. Biol. Chem. 272:11002.
  6. Gazziola, C. et al. (2005) Int. J. Oncol. 26:129.
  7. Kotenko, S.V. and S. Pestka (2000) Oncogene 19:2557.
  8. Lamken, P. et al. (2004) J. Mol. Biol. 341:303.
  9. Arduini, R.M. et al. (1999) Prot. Sci. 8:1867.
  10. Saleh, A.Z.M. et al. (2004) Oncogene 23:7076.
  11. McKenna, S.D. et al. (2004) J. Interferon Cytokine Res. 24:119.
  12. Tyler, D.R. et al. (2007) Mol. Immunol. 44:1864.
Long Name
Interferon alpha/beta Receptor 2
Entrez Gene IDs
3455 (Human); 15976 (Mouse); 102140083 (Cynomolgus Monkey)
Alternate Names
beta receptor10; human interferon alpha; human interferon alpha/beta receptor; IFNABR; IFN-alpha binding protein; IFN-alpha/beta R2; IFN-alpha/beta receptor 2; IFN-alpha-REC; IFNAR2; IFN-aR2; IFNARB; IFNbR2; IFN-bR2; IFN-R; IFN-R-2; interferon (alpha, beta and omega) receptor 2; Interferon alpha binding protein; interferon alpha/beta receptor 2; interferon-alpha/beta receptor beta chain; Type I interferon receptor 2

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