Recombinant Cynomolgus/Rhesus Glypican 3 His-tag Protein, CF

Catalog # Availability Size / Price Qty
10509-GP-050
Recombinant Cynomolgus/Rhesus Glypican 3 His-tag Protein, CF Binding Activity
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Recombinant Cynomolgus/Rhesus Glypican 3 His-tag Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human FGF basic/FGF2 (146aa) (Catalog # 233-FB/CF) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque Glypican 3 His-tag (Catalog # 10509-GP) binds with an ED50 of 0.075-0.45 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived Glypican 3 protein
Gln25-Asn554, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Gln25 (blocked) & Ser359
Predicted Molecular Mass
38 kDa (alpha chain), 23 kDa (beta chain), 61 kDa (full length)
SDS-PAGE
65-90 kDa, under non-reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10509-GP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Binding Activity View Larger

When Recombinant Human FGF basic/FGF2 (146 aa) Protein (233-FB/CF) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque Glypican 3 His-tag Protein (10509-GP) binds with an ED50 of 0.075‑0.45 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glypican 3

Glypican 3 (GPC3), also known as GTR2-2, intestinal protein OCI-5 and MXR7, is a member of the heparan sulfate (HS) proteoglycan family (1).  In mammals, six glypican family members have been identified, all sharing a conserved core protein that is linked to the cell membrane by a glycosylphosphatidylinositol (GPI) anchor. GPC3 can be cleaved by furin to produce two subunits that are linked by disulfide bonds: a 40 kDa N-terminal alpha subunit that can be secreted into the blood and a 30 kDa membrane-bound C-terminal beta subunit containing two HS glycan chains (1-3). Several isoforms of GPC3 due to alternative splicing have been reported (1). Within the mature extracellular domain, cynomolgus/rhesus GPC3 shares 99% amino acid sequence identity with human GPC3.  GPC3 is an important biomarker present in the serum of hepatocellular carcinoma patients, which distinguishes benign from cancerous nodules (4). Glypican 3 is over-expressed in hepatocellular carcinomas and binding of GPC3 to CD81 promotes development of carcinomas by activation of Hippo pathways in hepatocytes (5).  Our Avi-tag Biotinylated Recombinant  Glypican 3  features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide.  Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References
  1. Ho, M. and Kim, H. (2012) Eur. J. Cancer. 47:333.
  2. Haruyama, Y. and Kataoka, H. (2016) World J. Gastroenterol. 22:275.
  3. Shimizu, Y. et al. (2019) Front Oncol. 9:248.
  4. Ge, S. et al. (2018) Filmus, Int J Clin Exp Pathol. 11(12):5774.
  5. Xue, Y. et al. (2018) Am J Pathol. 188(6):1469.
Entrez Gene IDs
2719 (Human); 14734 (Mouse); 25236 (Rat); 102137748 (Cynomolgus Monkey)
Alternate Names
DGSX; Glypican 3; glypican proteoglycan 3; glypican-3; GPC3; GTR2-2; heparan sulphate proteoglycan; Intestinal protein OCI-5; MXR7; OCI5; OCI-5; secreted glypican-3; SGB; SGBS; SGBS1SDYS

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