Recombinant Human 2B4/CD244/SLAMF4 Fc Avi-tag Protein, CF

Biotinylated Fc Chimera
Catalog # Availability Size / Price Qty
AVI11050-050
Biotinylated Recombinant Human 2B4/CD244/SLAMF4 Fc Chimera Avi-tag Protein Binding Activity.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Recombinant Human 2B4/CD244/SLAMF4 Fc Avi-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD48/SLAMF2 Fc Chimera Protein (Catalog # 9310-CD) is immobilized at 1.0 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human 2B4/CD244/SLAMF4 Fc Chimera Avi-tag (Catalog # AVI1105) that produces 50% of the optimal binding response is 35.0‑350 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human 2B4/CD244/SLAMF4 protein
Human 2B4
(Cys22-Ile228)
Accession # NP_057466.1
IEGRMDHuman IgG1
(Pro100-Lys330)
Avi-tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Cys22
Structure / Form
Disulfide-linked homodimer
Biotinylated via Avi-tag
Predicted Molecular Mass
52 kDa
SDS-PAGE
68-85 kDa, under reducing conditions.

Product Datasheets

You must select a language.

x

AVI11050

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11050

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Binding Activity Biotinylated Recombinant Human 2B4/CD244/SLAMF4 Fc Chimera Avi-tag Protein Binding Activity. View Larger

When Recombinant Human CD48/SLAMF2 Fc Chimera Protein (9310-CD) is immobilized at 1.0 μg/mL, the concentration of Biotinylated Recombinant Human 2B4/CD244/SLAMF4 Fc Chimera Avi-tag Protein (Catalog # AVI1105) that produces 50% of the optimal binding response is 35.0‑350 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: 2B4/CD244/SLAMF4

2B4, also known as CD244 and SLAMF4, is a 66-kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 protein family. SLAM family proteins have an extracellular domain (ECD) with two or four Ig-like domains and at least two cytoplasmic immunoreceptor tyrosine-based switch motifs (ITSMs). 2B4 interacts with CD48, while other SLAM family proteins interact homophilically (1-4). Mature human 2B4 consists of a 208 amino acid (aa) ECD with two Ig-like domains, a 21 aa transmembrane segment, and a 120 aa cytoplasmic domain with four ITSMs (5, 6). Three additional splice variants of human 2B4 have deletions of the short region between the Ig-like domains, the second Ig-like domain, or a portion of the cytoplasmic tail. Within the ECD, human 2B4 shares 46% and 40% aa sequence identity with mouse and rat 2B4, respectively. The ECD of human 2B4 shares 17%-24% aa sequence identity with comparable regions of human CD2 family members BLAME, CD2F-10, CD84, CD229, CRACC, NTB-A, and SLAM. 2B4 is expressed on all NK cells, gamma δ T cells, monocytes, some CD4+ and CD8+ T cells, and some dendritic cells (7). CD48 mediates 2B4+ cell interactions with nearly all hematopoietic cell types, including cells of the same type (8-10). 2B4/CD48 signaling cooperates with other receptor systems to either promote or inhibit NK and CD8+ T cell activation (7-13). The inhibitory activities are distinct from those of MHC I restricted inhibitory NK cell receptors (12, 13). Ligation of 2B4 with antibodies or CD48 constructs can either directly trigger inhibitory signaling or disrupt an inhibitory interaction, leading to cellular activation (9, 12). The inhibitory effect is associated with the long form of 2B4, while the activation is associated with the short form (9, 14). 2B4 can also induce signaling through CD48 (10, 15). Our Avi-tag Biotinylated Recombinant Human 2B4 Fc Chimera protein features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References
  1. Bhat, R. et al. (2006) J. Leukoc. Biol. 79:417.
  2. Veillette, A. (2006) Nat. Rev. Immunol. 6:56.
  3. McNerney, M.E. et al. (2005) Mol. Immunol. 42:489.
  4. Assarsson, E. et al. (2005) J. Immunol. 175:2045.
  5. Boles, K.S. et al. (1999) Tissue Antigens 54:27.
  6. Kubin, M.Z. et al. (1999) Eur. J. Immunol. 29:3466.
  7. Nakajima, H. et al. (1999) Eur. J. Immunol. 29:1676.
  8. Lee, K.M. et al. (2006) Blood 107:3181.
  9. Mooney, J.M. et al. (2004) J. Immunol. 173:3953.
  10. Assarsson, E. et al. (2004) J. Immunol. 173:174.
  11. Bryceson, Y.T. et al. (2006) Blood 107:159.
  12. Lee, K-M. et al. (2004) J. Exp. Med. 199:1245.
  13. McNerney, M.E. et al. (2005) Blood 106:1337.
  14. Schatzle, J.D. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3870.
  15. Messmer, B. et al. (2006) J. Immunol. 176:4646.
Entrez Gene IDs
51744 (Human); 18106 (Mouse); 685808 (Rat); 102132700 (Cynomolgus Monkey)
Alternate Names
2B4; CD244 antigen; CD244 molecule, natural killer cell receptor 2B4; CD244; h2B4; NAIL; NAILNK cell activation-inducing ligand; natural killer cell receptor 2B4; NKR2B4; NKR2B4NK cell type I receptor protein 2B4,2B4CD244 natural killer cell receptor 2B4; Nmrk; SLAMF4; SLAMF4NK cell activation inducing ligand NAIL

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human 2B4/CD244/SLAMF4 Fc Avi-tag Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human 2B4/CD244/SLAMF4 Fc Avi-tag Protein, CF and earn rewards!

Have you used Recombinant Human 2B4/CD244/SLAMF4 Fc Avi-tag Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review