Recombinant Human Adiponectin/Acrp30 Protein, CF

R&D Systems | Catalog # 1065-AP

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human Adiponectin/Acrp30 Protein (1065-AP)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q15848

Structure / Form

Oligomer

Applications

Binding Activity
View all Adiponectin/Acrp30 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Adiponectin/Acrp30 protein
Glu19-Asn244, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu19

Predicted Molecular Mass

25.4 kDa

SDS-PAGE

27-35 kDa, reducing conditions

Activity

Measured by its ability to induce TIMP-1 secretion by mouse macrophages. Kumada, M. et al. (2004) Circulation 109:2046.
The ED50 for this effect is 1.20-18.0 µg/mL.

Reviewed Applications

Read 2 reviews rated 5 using 1065-AP in the following applications:

Formulation, Preparation, and Storage

1065-AP
Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: Adiponectin/Acrp30

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It is induced during adipocyte differentiation, and its secretion is stimulated by insulin. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, Adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1-3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors (4-7). Mature human Adiponectin consists of a 60 amino acid (aa) N‑terminal collagenous region and a 137 aa C‑terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8-9). Mature human Adiponectin shares 83% and 85% amino acid (aa) sequence identity with mouse and rat Adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into >300 kDa high molecular weight (HMW) oligomers (10-12). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (13). The various multimeric forms of Adiponectin exhibit distinct tissue specific and gender specific profiles and activities (12, 14).

References

  1. Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263.
  2. Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772.
  3. Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326.
  4. Yamauchi, T. et al. (2007) Nat. Med. 13:332.
  5. Takemura, Y. et al. (2007) J. Clin. Invest. 117:375.
  6. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308.
  7. Wang, Y. et al. (2005) J. Biol. Chem. 280:18341.
  8. Maeda, K. et al. (1996) Biochem. Biophys. Res. Commun. 221:286.
  9. Waki, H. et al. (2005) Endocrinology 146:790.
  10. Waki, H. et al. (2003) J. Biol. Chem. 278:40352.
  11. Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.
  12. Wang, Y. et al. (2008) Biochem. J. 409:623.
  13. Wang, H. et al. (2006) J. Biol. Chem. 281:16391.
  14. Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.

Alternate Names

Acrp30, AdipoQ, ApM1, GBP28

Entrez Gene IDs

9370 (Human); 11450 (Mouse); 246253 (Rat)

Gene Symbol

ADIPOQ

UniProt

Q15848

Additional Adiponectin/Acrp30 Products

Product Documents for Recombinant Human Adiponectin/Acrp30 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Adiponectin/Acrp30 Protein, CF

For research use only

Citations for Recombinant Human Adiponectin/Acrp30 Protein, CF

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  • Recombinant Human Adiponectin/Acrp30 Protein, CF
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 09/04/2019
    Recombinant Human Adiponectin/Acrp30 Protein, CF 1065-AP
  • Recombinant Human Adiponectin/Acrp30 Protein, CF
    Name: Balaji Mahender
    Application: Immunoassay Standard
    Verified Customer | Posted 12/20/2017

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