>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt‑3 Ligand (Catalog # 427-FL). The ED50 for this effect is typically 100-600 ng/mL in the presence of a cross-linking antibody, His Tag MAb,
Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
Mouse myeloma cell line, NS0-derived Gly26-Ser406 (Lys163Ala, Arg164Ala), with a C-terminal 6-His tag
Both Biotinylated Recombinant Human Angiopoietin-like Protein 4/ANGPTL4 (Catalog # BT4487) and unlabeled Recombinant Human Angiopoietin-like Protein 4/ANGPTL4 (Catalog # 4487-AN) promotes the expansion of E16-E18 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c-kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt-3 Ligand (Catalog # 427-FL). The ED50 for this effect is typically 100-600 ng/mL in the presence of a cross-linking antibody, His Tag MAb (Catalog # MAB050). The similarity in activity highlights that the biotinylated protein is fully functional.
Background: Angiopoietin-like Protein 4/ANGPTL4
Angiopoietin-like 4 (ANGPTL4), also known as FIAF, FARP, and PGAR, is a 55 kDa glycoprotein secreted by the liver and fat tissue. It is structurally related to the angoipoietins and contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain which can be proteolytically separated in vivo (1). Mature human ANGPTL4 shares 26% - 30% amino acid (aa) sequence identity with ANGPTL1, 2, 3, 5, 6, and 7. It shares approximately 75% aa sequence identity with mouse and rat ANGPT-L4. The coiled coil domain, which is not glycosylated, mediates the formation of variable sized disulfide-linked oligomers (2). This domain directly inhibits lipoprotein lipase, resulting in increased circulating triglyceride levels (3, 4). In humans, the N-terminal fragment and full length ANGPTL4 physically associate with HDL (4). In mouse, however, full length ANGPTL4 associates with HDL, while the N-terminal fragment associates with LDL (4). Circulating ANGPTL4 is decreased in type II diabetics with a subsequent loss of its normal plasma glucose lowering activity (5). Its expression in adipose tissue is induced by fasting and suppressed by feeding (6). In hypoxic areas, ANGPTL4 is induced in both vascular endothelial cells and tumor cells (7, 8). The N-terminal fragment can function as an angiogenesis inhibitor (7, 8). In contrast, the C-terminal fragment modulates cell adhesion through interactions with heparan sulfate proteoglycans, Integrins beta 1 and beta 5, Vitronectin, and Fibronectin, thereby promoting keratinocyte migration and wound healing (7, 9, 10). ANGPTL4 additionally enhances the survival of hematopoietic and mesenchymal stem cells (11, 12). The expression of an undersialylated form of ANGPTL4 in renal podocytes contributes to proteinuria and nephrotic syndrome (13).
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Sukonina, V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:17450.
Mandard, S. et al. (2006) J. Biol. Chem. 281:934.
Xu, A. et al. (2005) Proc. Natl. Acad. Sci. USA 102:6086.
Kersten, S. et al. (2000) J. Biol. Chem. 275:28488.
Cazes, A. et al. (2006) Circ. Res. 99:1207.
Le Jan, S. et al. (2003) Am. J. Pathol. 162:1521.
Goh, Y.Y. et al. (2010) Am. J. Pathol. 177:2791.
Goh, Y.Y. et al. (2010) J. Biol. Chem. 285:32999.
Blank, U. et al. (2012) Eur. J. Haematol. 89:198.
Hou, M. et al. (2014) PLoS ONE 9:e85808.
Clement, L.C. et al. (2011) Nat. Med. 17:117.
Entrez Gene IDs:
51129 (Human); 57875 (Mouse); 362850 (Rat)
Angiopoietin like Protein 4; angiopoietin-like 4; Angiopoietin-like protein 4; ANGPTL4; ARP4fasting-induced adipose factor; FIAF; FIAFhepatic angiopoietin-related protein; Hepatic fibrinogen/angiopoietin-related protein; HFARP; HFARPANGPTL2; NL2; peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein; PGAR; PGARangiopoietin-related protein 4; pp1158; PPARG angiopoietin related protein
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