Recombinant Human Apolipoprotein A-I Protein, CF Summary
Asp25-Gln267, with an N-terminal Met and 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Apolipoprotein A-I/ApoA1
Apolipoprotein A1 (ApoA1) is a 28 kDa glycoprotein that is the major protein component of high density lipoprotein (HDL) particles. HDL particles play a central role in the reverse transport of cholesterol from peripheral tissues to the liver. HDL is known as “good cholesterol” due to its ability to facilitate the removal of cholesterol from macrophage foam cells in atherosclerotic plaques and thereby retard the progression of disease (1, 2). ApoA1 is secreted from hepatocytes with variable amounts of lipidation (3). Disc‑like HDL particles increase in size and adopt a spherical shape as they are loaded with additional lipids and cholesterol by ABC family transporters (4, 5). HDL particle interaction with lipid‑supplying cells is mediated by ApoA1 binding to the scavenger receptor SR‑A1 (6). HDL particles are further modified by the circulating enzymes LCAT (which converts cholesterol to cholesteryl esters), CETP (which transfers cholesteryl esters to LDL/VLDL particles), and PLTP (which transfers phospholipids from HDL to LDL/VLDL particles) (2). Upon HDL particle return to the liver, ApoA1 binds to the scavenger receptor SR‑B1 and the beta chain of ATP synthase on hepatocytes (7, 8). Hepatocytes internalize the particles and pass the cholesterol into bile for excretion. Polymorphisms of ApoA1 are associated with dysregulation of HDL levels and cholesterol homeostasis (1, 9). ApoA1 is catabolized in the kidney following its binding to Cubulin on renal proximal tubule epithelial cells (10). ApoA1, either as a free molecule or in lipidated particulate form, induces the release of insulin from pancreatic islets in a process that is dependent on ABCA1 and SR‑B1 (11). Mature human ApoA1 shares 65% and 62% aa sequence identity with mouse and rat ApoA1, respectively.
- Obici, L. et al. (2006) Amyloid 13:191.
- Annema, W. and U.J.F. Tietge (2012) Nutr. Metab. 9:25.
- Chisholm, J.W. et al. (2002) J. Lipid Res. 43:36.
- Wang, N. et al. (2004) Proc. Natl. Acad. Sci. USA 101:9774.
- Kiss, R.S. et al. (2003) J. Biol. Chem. 278:10119.
- Neyen, C. et al. (2009) Biochemistry 48:11858.
- Williams, D.L. et al. (2000) J. Biol. Chem. 275:18897.
- Martinez, L.O. et al. (2003) Nature 421:75.
- Cohen, J.C. et al. (2004) Science 305:869.
- Kozyraki, R. et al. (1999) Nat. Med. 5:656.
- Fryirs, M.A. et al. (2010) Arterioscler. Thromb. Vasc. Biol. 30:1642.
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