Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
193-BC-050
Graph showing dose-dependent bioactivity of Human BCMA protein
1 Image
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (2)

Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit APRIL-mediated proliferation of anti-IgM stimulated mouse B cells. Moore, P.A. et al. (1999) Science 285:260; Gross, J.A. et al. (2000) Nature 404:995; Schneider, P. et al. (1999) J. Exp. Med. 189:1747. The ED50 for this effect is 0.01-0.04 µg/mL in the presence of 100 ng/mL of rhAPRIL.
Source
Mouse myeloma cell line, NS0-derived human BCMA/TNFRSF17 protein
Human BCMA
(Met1-Ala54)
Accession # Q6PE46
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Met1
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
32 kDa (monomer)
SDS-PAGE
35-45 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

193-BC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Bioactivity of Human BCMA / TNFRSF17 Protein View Larger

Recombinant human BCMA / TNFRSF17 (193-BC) inhibits APRIL-mediated proliferation of anti-IgM stimulated mouse B cells. The ED50 for this effect is 0.01-0.4 µg/mL in the presence of 100 ng/mL of recombinant human APRIL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BCMA/TNFRSF17

BCMA, B cell maturation antigen, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.

References
  1. Madry, C. et al. (1998) Int. Immunol. 10:1693.
  2. Gras, M. et al. (1995) Int. Immunol. 7:1093.
  3. Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
  4. Marsters, S. et al. (2000) Curr. Biol. 10:785.
  5. Thompson, J. et al. (2000) J. Exp. Med. 192:129.
Long Name
B Cell Maturation Factor
Entrez Gene IDs
608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)
Alternate Names
B cell maturation antigen; B-cell maturation protein; BCMA; BCMAtumor necrosis factor receptor superfamily member 17; BCMB-cell maturation factor; CD269 antigen; CD269; TNFRSF13A; TNFRSF17; tumor necrosis factor receptor superfamily, member 17

Citations for Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Generation and validation of structurally defined antibody-siRNA conjugates
    Authors: AR Nanna, AV Kel'in, C Theile, JM Pierson, ZX Voo, A Garg, JK Nair, MA Maier, K Fitzgerald, C Rader
    Nucleic Acids Res., 2020;0(0):.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Capture
  2. Pre-clinical validation of B cell maturation antigen (BCMA) as a target for T cell immunotherapy of multiple myeloma
    Authors: DX Bu, R Singh, EE Choi, M Ruella, S Nunez-Cruz, KG Mansfield, P Bennett, N Barton, Q Wu, J Zhang, Y Wang, L Wei, S Cogan, T Ezell, S Joshi, KJ Latimer, B Granda, WR Tschantz, RM Young, HA Huet, CJ Richardson, MC Milone
    Oncotarget, 2018;9(40):25764-25780.
    Species: Human
    Sample Types: Protein
    Applications: CAR-T (SPR)
  3. Enhanced immunogenicity of HIV-1 envelope gp140 proteins fused to APRIL.
    Authors: Isik G, Sliepen K, van Montfort T, Sanders R
    PLoS ONE, 2014;9(9):e107683.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay

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Reviews for Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF

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Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF
By Anonymous on 12/13/2019
Application: SEC
Reason for Rating: 97% monomer

Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF
By Anonymous on 11/05/2018
Application: Binding assay/Protein-protein interaction