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Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF

R&D Systems | Catalog # 193-BC

Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems NS0-derived Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein (193-BC)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q6PE46

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all BCMA/TNFRSF17 Proteins and Enzymes »

Why choose R&D Systems BCMA Protein?

  • Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 95%.
  • Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
  • Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
  • Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.

NEW! Fluorescent labeled BCMA designed for validating expression of a BCMA chimeric antigen receptor (CAR). Fluorescent BCMA Proteins

Avi-tag BCMA protein is also available with the same activity as the unlabeled BCMA protein and consistent biotinylation Avi-tag BCMA

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human BCMA/TNFRSF17 protein
Human BCMA
(Met1-Ala54)
Accession # Q6PE46		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1

Predicted Molecular Mass

32 kDa (monomer)

SDS-PAGE

35-45 kDa, reducing conditions

Activity

Measured by its ability to inhibit APRIL-mediated proliferation of anti-IgM stimulated mouse B cells. Moore, P.A. et al. (1999) Science 285:260; Gross, J.A. et al. (2000) Nature 404:995; Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
The ED50 for this effect is 0.0100-0.100 µg/mL in the presence of 100 ng/mL of rhAPRIL.

Reviewed Applications

Read 4 reviews rated 5 using 193-BC in the following applications:

Scientific Data Images for Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF

Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein SEC-MALS.

Recombinant Human BCMA/TNFRSF17 Fc Chimera (Catalog # 193-BC) has a molecular weight (MW) of 70.6 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Graph showing dose-dependent bioactivity of Human BCMA protein

Bioactivity of Human BCMA / TNFRSF17 Protein

Recombinant human BCMA / TNFRSF17 (193-BC) inhibits APRIL-mediated proliferation of anti-IgM stimulated mouse B cells. The ED50 for this effect is 0.0100-0.100 µg/mL in the presence of 100 ng/mL of recombinant human APRIL.

Formulation, Preparation, and Storage

193-BC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BCMA/TNFRSF17

BCMA, B cell maturation antigen, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.

References

  1. Madry, C. et al. (1998) Int. Immunol. 10:1693.
  2. Gras, M. et al. (1995) Int. Immunol. 7:1093.
  3. Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
  4. Marsters, S. et al. (2000) Curr. Biol. 10:785.
  5. Thompson, J. et al. (2000) J. Exp. Med. 192:129.

Long Name

B Cell Maturation Factor

Alternate Names

CD269, TNFRSF13A, TNFRSF17

Entrez Gene IDs

608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)

Gene Symbol

TNFRSF17

UniProt

Q6PE46

Additional BCMA/TNFRSF17 Products

Product Documents for Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF

Customer Reviews for Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF (4)

5 out of 5
4 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Showing  1 - 4 of 4 reviews Showing All
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  • Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Octet BLI (Biolayer Interferometry)
    Verified Customer | Posted 07/22/2025
  • I recommend it
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 09/09/2024
    ELISA signal
    The protein worked well for my ELISA. Same for AVI193. My experiment confirmed the binding of 193-BC and 2149-BF as indicated in the blue colour change in the attached image. I was testing a certain compound that has the potential to inhibit the binding of BCMA with its ligands. I didn't see any difference between 193-BC and AVI193, both binds to rhBAFF and produced similar signals.
    Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF 193-BC
  • Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF
    Name: Anonymous
    Application: SEC
    Verified Customer | Posted 12/13/2019
    Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF 193-BC
  • Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 11/05/2018

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