Recombinant Human CD19 Protein, Atto 488 Conjugate

Detection of CD19 construct on human CART cells

Human PBMC CD4+ and CD8+ T cells were either (A) transduced or (B) not transduced (negative control) with a Human CD19 CAR and then cultured for 11 days. Cells were washed twice with 2mL 1x PBS then stained with a viability dye for 15-30 min at room temp in the dark. Human block (1-001-A) was added during the viability stain to block Fc receptors. Cells were washed twice with Flow Cytometry Staining Buffer (FC001) then stained with Human CD4 PE-Cy7-conjugated Antibody and rhCD19 Atto 488-conjugated protein (ATJ9269) for 25 min at room temp in the dark. Staining was performed using our staining membrane proteins protocol.

Recombinant Human CD19 Protein, Atto 488 Conjugate SDS-PAGE.

2 μg/lane of Recombinant Human CD19 Fc Chimera Atto 488 (ATJ9269) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 77-92 kD and 154-184 kDa, respectively.