Recombinant Human CD8 alpha Fc Chimera Avi-tag Protein, CF

Name: Recombinant Human CD8 alpha Fc Chimera Avi-tag Protein, CF
Brand: R&D Systems
SKU: AVI10927
Price: 742 USD
Availability: InStock

R&D Systems | Catalog # AVI10927

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

R&D Systems CHO-derived Recombinant Human CD8 alpha Fc Chimera Avi-tag Protein (AVI10927)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P01732.1

Structure / Form

Biotinylated via Avi-tag.

Conjugate

Biotin

Applications

Bioactivity

View all CD8 Proteins and Enzymes »

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD8 protein

Human CD8 alpha (Ser22-Asp182) Accession # P01732.1	IEGRMD	Human IgG1 (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser22

Predicted Molecular Mass

46 kDa

SDS-PAGE

55-61 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Human CD8 alpha Antibody (Catalog # MAB3803) is immobilized at 2 μg/mL, 100 μg/mL, Biotinylated Recombinant Human CD8 alpha Fc Chimera Avi-tag binds with an ED₅₀ of 2.00-12.0 ng/mL.

Scientific Data Images for Recombinant Human CD8 alpha Fc Chimera Avi-tag Protein, CF

When Human CD8 alpha Antibody (Catalog # MAB3803) is immobilized at 2 μg/mL, 100 μg/mL, Biotinylated Recombinant Human CD8 alpha Fc Chimera Avi-tag (AVI10927) binds with an ED₅₀ of 2.00-12.0 ng/mL.

Biotinylated Recombinant Human CD8 alpha Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human CD8 alpha Fc Chimera Avi-tag Protein (Catalog # AVI10927) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-61 kDa and 110-120 kDa, respectively.

Formulation, Preparation, and Storage

AVI10927

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD8

CD8, also known as Ly2 or Leu2, is a heterodimeric glycoprotein (alpha and beta subunits) that functions in conjunction with the T cell receptor in the recognition of MHC class I/peptide complexes (1, 2). CD8 alpha is expressed on double positive (CD4+ CD8+) thymocytes and mature CD8+ cytolytic T cells (CTL) (3-5), intraepithelial lymphocytes (IEL) (6), some gamma delta T cells (7), subsets of thymic and splenic dendritic cells (DC) (8), and peritoneal mast cells (9). It can form disulfide linked homodimers or heterodimers with CD8 beta (10). Thymic CD8+ DC express primarily alpha beta heterodimers, while splenic CD8+ DC primarily express alpha alpha homodimers (8). CD8 alpha + DC can present viral antigenic peptides in complex with MHC I and prime CTL responses (11). The approximately 35 kDa mature mouse CD8 alpha consists of a 169 amino acid (aa) extracellular domain (ECD) with one Iglike domain, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain (12). Within the ECD, mouse CD8 alpha shares 49% and 64% aa sequence identity with human and rat CD8 alpha, respectively. Our Avi-tag Biotinylated human CD8 alpha features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of bionylation and the rest of the protein is unchanged so there is no interference in the protein bioactivity.