Recombinant Human DNAM-1/CD226 His-tag Avi-tag Protein, CF

Biotinylated
Catalog # Availability Size / Price Qty
AVI9298-050
Recombinant Human DNAM-1/CD226 His-tag Avi-tag Protein, CF Binding Activity
2 Images
Product Details
FAQs
Supplemental Products
Reviews

Recombinant Human DNAM-1/CD226 His-tag Avi-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When 250 ng/mL of Biotinylated Recombinant Human DNAM-1/CD226 His-tag Avi-tag (AVI9298) protein is immobilized onto Streptavidin coated plate (Catalog # CP004), it binds to Recombinant Human CD155/PVR Fc Chimera (Catalog # 9174-CD) with an ED50 of 20-100 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human DNAM-1/CD226 protein
Human DNAM-1
(Glu19-Asn224)
Accession # Q15762
HHHHHHAvi-tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Glu19
Structure / Form
Biotinylated protein via Avi-tag
Predicted Molecular Mass
29 kDa
SDS-PAGE
35-60 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI9298

Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Data Images

Binding Activity Binding Activity View Larger

When 250 ng/mL of Biotinylated Recombinant Human DNAM-1/CD226 His-tag Avi-tag protein (Catalog # AVI9298) is immobilized onto a Streptavidin coated plate (Catalog # CP004), it binds to Recombinant Human CD155/PVR Fc Chimera (Catalog # 9174-CD) with an ED50 of 20-100 ng/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human DNAM-1/CD226 His-tag Avi-tag (Catalog # AVI9298) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR)conditions and visualized by silver staining, showing bands at 35-60 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: DNAM-1/CD226

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155/PVR and Nectin-2/CD112 (3, 4). Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells (2-6), dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the formation of osteoclasts (7-10). Platelet-endothelium interactions mediated by DNAM-1 can enable the metastasis of tumor cells to the lung (11). CD96 competes with DNAM-1 for binding to CD155 and blocks DNAM-1 mediated NK cell activation (12). In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation (6, 9, 13-15).

References
  1. Zingoni, A. et al. (2013) Front. Immunol. 3:408.
  2. Shibuya, A. et al. (1996) Immunity 4:573.
  3. Bottino, C. et al. (2003) J. Exp. Med. 198:557.
  4. Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
  5. Dardalhon, V. et al. (2005) J. Immunol. 175:1558.
  6. Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190.
  7. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
  8. Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209.
  9. Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.
  10. Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
  11. Morimoto, K. et al. (2007) Oncogene 27:264.
  12. Chan, C.J. et al. (2014) Nat. Immunol. 15:431.
  13. Shibuya, K. et al. (1999) Immunity 11:615.
  14. Shibuya, K. et al. (2003) J. Exp. Med. 198:1829.
  15. Shibuya, A. et al. (1998) J. Immunol. 166:1671.
Long Name
DNAX Accessory Molecule 1
Entrez Gene IDs
10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)
Alternate Names
CD226 antigenplatelet and T cell activation antigen 1; CD226 molecule; CD226; DNAM1; DNAM-1; DNAM1adhesion glycoprotein; DNAM-1DNAX accessory molecule-1; DNAX accessory molecule 1; PTA1; T lineage-specific activation antigen 1 antigen; TLiSA1

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human DNAM-1/CD226 His-tag Avi-tag Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human DNAM-1/CD226 His-tag Avi-tag Protein, CF and earn rewards!

Have you used Recombinant Human DNAM-1/CD226 His-tag Avi-tag Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review