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Recombinant Human E-Cadherin Fc Chimera Protein, CF

Catalog #: 648-EC
10 Citations 2 Reviews Datasheet / COA / SDS
Newer Version Available: 8505-EC
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Discontinued Product

648-EC has been discontinued and is replaced by 8505-EC.

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Citations (10)
FAQs
Reviews (2)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human E-Cadherin Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of the MCF‑7 human breast cancer cells. When 5 x 104 cells/well are added to Recombinant Human E-Cadherin Fc Chimera coated plates (1.5 µg/mL with 100 µL/well), >60% cells will adhere after 90 minutes at 37 °C.
Optimal concentration depends on cell type as well as the application or research objectives.
Source
Mouse myeloma cell line, NS0-derived human E-Cadherin protein
Human E-Cadherin
(Asp155-Ile707)
Accession # NP_004351		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus
Accession #
NP_004351
N-terminal Sequence
Analysis
Asp155
Predicted Molecular Mass
87.7 kDa (monomer)
SDS-PAGE
120 kDa, reducing conditions

Product Datasheets

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648-EC

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

648-EC

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate, NaCl and CaCl2.
Reconstitution Reconstitute at 100 μg/mL in sterile DPBS with Ca2+ and Mg2+.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: E-Cadherin

E-Cadherin/Cadherin-1, also known as Uvomorulin in the mouse and rat, is a 120 kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion (1). Human E-Cadherin shares 81% amino acid sequence identity with the rat and mouse proteins (2). It is a single-pass transmembrane protein that mediates calcium-dependent epithelial cell adhesion. E-Cadherin has five extracellular EC domains that form homophilic cis-clusters between adjacent epithelial cells and trans-clusters within the same cell. E-Cadherin clusters are critical components of adherens junctions between epithelial cells and act in the formation and maintenance of the epithelial cell barrier (3, 4). The intracellular domain of E-Cadherin binds to the Catenin cytoskeletal complex, which includes p120 Catenin, beta‑Catenin, alpha-Catenin, and Vinculin. E-Cadherin expression is critical for epithelial tissue homeostasis. Decreased E-Cadherin is associated with physiological and pathological epithelial-to-mesenchymal transition and cell migration, and E-Cadherin loss contributes to cancer metastasis (5). The extracellular E-Cadherin N‑terminal domain can be cleaved by several proteases and is released as a soluble factor that enhances cancer cell motility and EGFR-dependent survival and proliferation (6).

 

References
  1. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
  2. Bussemakers, M.J. et al. (1993) Mol. Biol. Rep. 17:123.
  3. Guillot, C. and T. Lecuit (2013) Science 340:1185.
  4. Tian, X. et al. (2011) J. Biomed. Biotechnol. 2011:567305.
  5. Stemmler, M.P. (2008) Mol. Biosyst. 4:835.
  6. David, J.M. and A.K. Rajasekaran (2012) Cancer Res. 72:2917.
Entrez Gene IDs
999 (Human); 12550 (Mouse); 83502 (Rat)
Alternate Names
Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin

Citations for Recombinant Human E-Cadherin Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Alternative molecular mechanisms for force transmission at adherens junctions via ?-catenin-vinculin interaction
    Authors: Morales-Camilo, N;Liu, J;Ramírez, MJ;Canales-Salgado, P;Alegría, JJ;Liu, X;Ong, HT;Barrera, NP;Fierro, A;Toyama, Y;Goult, BT;Wang, Y;Meng, Y;Nishimura, R;Fong-Ngern, K;Low, CSL;Kanchanawong, P;Yan, J;Ravasio, A;Bertocchi, C;
    Nature communications
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Functional Assay
  2. Loss of ER? induces amoeboid-like migration of breast cancer cells by downregulating vinculin
    Authors: Y Gao, Z Wang, Q Hao, W Li, Y Xu, J Zhang, W Zhang, S Wang, S Liu, M Li, X Xue, W Zhang, C Zhang, Y Zhang
    Nat Commun, 2017-03-07;8(0):14483.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Stromal cells positively and negatively modulate the growth of cancer cells: stimulation via the PGE2-TNFalpha-IL-6 pathway and inhibition via secreted GAPDH-E-cadherin interaction.
    Authors: Kawada M, Inoue H, Ohba S, Yoshida J, Masuda T, Yamasaki M, Usami I, Sakamoto S, Abe H, Watanabe T, Yamori T, Shibasaki M, Nomoto A
    PLoS ONE, 2015-03-18;10(3):e0119415.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  4. A role for IL-25 and IL-33-driven type-2 innate lymphoid cells in atopic dermatitis.
    Authors: Salimi M, Barlow J, Saunders S, Xue L, Gutowska-Owsiak D, Wang X, Huang L, Johnson D, Scanlon S, McKenzie A, Fallon P, Ogg G
    J Exp Med, 2013-12-09;210(13):2939-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. MMP20 modulates cadherin expression in ameloblasts as enamel develops.
    Authors: Guan X, Bartlett J
    2013-09-25;92(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Distinct PTPmu-associated signaling molecules differentially regulate neurite outgrowth on E-, N-, and R-cadherin.
    Authors: Oblander SA, Brady-Kalnay SM
    Mol. Cell. Neurosci., 2010-03-01;44(1):78-93.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: Bioassay
  7. HIV-1 envelope protein binds to and signals through integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.
    Authors: Arthos J, Cicala C, Martinelli E, MacLeod K, Van Ryk D, Wei D, Xiao Z, Veenstra TD, Conrad TP, Lempicki RA, McLaughlin S, Pascuccio M, Gopaul R, McNally J, Cruz CC, Censoplano N, Chung E, Reitano KN, Kottilil S, Goode DJ, Fauci AS
    Nat. Immunol., 2008-02-10;9(3):301-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. E-cadherin promotes retinal ganglion cell neurite outgrowth in a protein tyrosine phosphatase-mu-dependent manner.
    Authors: Oblander SA, Ensslen-Craig SE, Longo FM, Brady-Kalnay SM
    Mol. Cell. Neurosci., 2007-02-02;34(3):481-92.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: Bioassay
  9. Distinct roles for cysteine cathepsin genes in multistage tumorigenesis.
    Authors: Gocheva V, Zeng W, Ke D, Klimstra D, Reinheckel T, Peters C, Hanahan D, Joyce JA
    Genes Dev., 2006-02-15;20(5):543-56.
    Species: Human
    Sample Types: Buffer
    Applications: Enzyme Assay
  10. Lamellipodium extension and cadherin adhesion: two cell responses to cadherin activation relying on distinct signalling pathways.
    Authors: Gavard J, Lambert M, Grosheva I, Marthiens V, Irinopoulou T, Riou JF, Bershadsky A, Mege RM
    J. Cell. Sci., 2003-12-02;117(0):257-70.
    Species: Chicken, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human E-Cadherin Fc Chimera Protein, CF

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Recombinant Human E-Cadherin Fc Chimera Protein, CF
By Anonymous on 11/30/2018
Application: Cell Adhesion
Recombinant Human E-Cadherin Fc Chimera Protein, CF
By Anonymous on 07/20/2016
Application: Binding assay/Protein-protein interaction
Reason for Rating: Give nice ELISA binding curves for positive antibodies.