Fc gamma RIIIa is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity and clearance of immune complexes (1, 2). In humans, it is a 50-70 kDa type I transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages (1). Fc gamma RIIIb is highly related, sharing 97% amino acid (aa) identity within the extracellular domain (ECD), but is a GPI-linked receptor expressed on human neutrophils and eosinophils (1, 2). The ECD of Fc gamma RIIIa shares 63%, 61%, 65%, 59% and 58% aa identity with mouse Fc gamma RIV, rat Fc gamma RIIIa, feline CD16, bovine CD16 and porcine Fc gamma RIIIb paralogs, respectively. The Fc gamma RIIIa cDNA encodes 254 aa including a 16 aa signal sequence, 191 aa ECD with two C2-type Ig-like domains and five potential N-glycosylation sites, a 22 aa transmembrane (TM) sequence and a 25 aa cytoplasmic domain. In humans, a single nucleotide polymorphism creates high binding (176V) and low binding (176F) forms that, when homozygous, may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies (3, 4). Catalog # 4325-FC is expressed as the 176V isoform of Fc gamma RIIIa. Fc gamma RIIIa surface expression requires interaction of an accessory chain, either the common gamma -chain or CD3 zeta (5, 6). Glycosylation patterns, electrophoretic mobility and binding affinity appear to differ between NK cell and monocyte Fc gamma RIIIa (7). The ECD of both Fc gamma RIIIa and b can be proteolytically cleaved and retain binding activity in soluble form (8-11). In monocytes and macrophages, activation and phagocytosis can trigger Fc gamma RIIIa release (11). Soluble Fc gamma RIII can be detected in normal plasma and is increased in rheumatoid arthritis and in coronary artery diseases (9, 10).
Recombinant Human Fc gamma RIIIA/CD16a Protein, CF
R&D Systems | Catalog # 4325-FC
Key Product Details
- R&D Systems NS0-derived Recombinant Human Fc gamma RIIIA/CD16a Protein (4325-FC)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Applications
Why choose R&D Systems Fc gamma RIIIA Protein?
- Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 90%.
- Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
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- Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.
Product Specifications
Source
Gly17-Gln208, with a C-terminal 6-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
Reviewed Applications
Read 7 reviews rated 5 using 4325-FC in the following applications:
Scientific Data Images for Recombinant Human Fc gamma RIIIA/CD16a Protein, CF
Recombinant Human Fc gamma RIIIA/CD16a Protein SEC-MALS.
Recombinant Human Fc gamma RIIIA/CD16a Protein (Catalog # 4325-FC) has a molecular weight (MW) of 33.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Recombinant Human Fc gamma RIIIA/CD16a Protein Binding Activity
Recombinant Human Fc gamma RIIIA/CD16a (Catalog # 4325-FC) binds human IgG with an estimated Kd<50 nM.
Recombinant Human Fc gamma RIIIA/CD16a Protein SDS-PAGE
1 μg/lane of Recombinant Human Fc gamma RIIIA/CD16a was resolved with SDS‑PAGE under reducing (R) conditions and visualized by silver staining, showing bands at 40-50 kDa.Formulation, Preparation, and Storage
4325-FC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Calculators
Background: Fc gamma RIIIA/CD16a
References
- Nimmerjahn, F. and J.V. Ravetch (2006) Immunity 24:19.
- Ravetch, J.V. and B. Perussia (1989) J. Exp. Med. 170:481.
- Wu, J. et al. (1997) J. Clin. Invest. 100:1059.
- Dall’Ozzo, S. et al. (2004) Cancer Res. 64:4664.
- Kim, M.-K. et al. (2003) Blood 101:4479.
- Lanier, L.L. et al. (1989) Nature 342:803.
- Edberg, J.C. and R.P. Kimberley (1997) J. Immunol. 159:3849.
- Li, P. et al. (2007) J. Biol. Chem. 282:6210.
- Masuda, M. et al. (2003) J. Rheumatol. 30:1911.
- Masuda, M. et al. (2006) Atherosclerosis 188:377.
- Webster, N.L. et al. (2006) J. Leukoc. Biol. 79:294.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional Fc gamma RIIIA/CD16a Products
Product Documents for Recombinant Human Fc gamma RIIIA/CD16a Protein, CF
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Fc gamma RIIIA/CD16a Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human Fc gamma RIIIA/CD16a Protein, CF
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Application: Immunoassay StandardVerified Customer | Posted 07/22/2025
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Application: Immunoassay StandardVerified Customer | Posted 03/25/2019
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 03/06/2019
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 10/24/2018
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Application: Enzymatic activity in vitroVerified Customer | Posted 10/12/2018
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Application: Apoptosis assayVerified Customer | Posted 07/11/2018
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Sample Tested: Human recombinant test antibodySpecies: HumanVerified Customer | Posted 10/26/2015Specificity: Specific<br />Sensitivity: Sensitive<br />Buffer: TBS + Tween + Milk<br />Dilution: used at 0.25 ug/mL
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FAQs for Recombinant Human Fc gamma RIIIA/CD16a Protein, CF
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Q: Is Recombinant Human Fc gamma RIIIA/CD16a Protein, CF (Catalog # 4325-FC) the low binding or high binding form?
A: Catalog # 4325-FC has Valine at amino acid position 176, making it the high binding form. We also offer the low binding form, with 176F, as catalog # 8894-FC.
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Q: What is the amino acid sequence of Recombinant Human Fc gamma RIIIA/CD16a Protein, CF (Catalog # 4325-FC)?
A: The amino acid sequence is Gly17-Gln208, with a C-terminal 6-His tag, from Accession # AAH17865. This includes Tyr158 and Val176.
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Q: Is Recombinant Human Fc gamma RIIIA/CD16a Protein, CF (Catalog # 4325-FC) the low binding or high binding form?
A: Catalog # 4325-FC has Valine at amino acid position 176, making it the high binding form. We also offer the low binding form, with 176F, as catalog # 8894-FC.
-
Q: What is the amino acid sequence of Recombinant Human Fc gamma RIIIA/CD16a Protein, CF (Catalog # 4325-FC)?
A: The amino acid sequence is Gly17-Gln208, with a C-terminal 6-His tag, from Accession # AAH17865. This includes Tyr158 and Val176.