Recombinant Human Fc gamma RIIIA/CD16a Protein, CF

(7 citations)
(1 Review)
  • Purity
    >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to bind human IgG with an estimated Kd <50 nM.
  • Source
    Mouse myeloma cell line, NS0-derived Gly17-Gln208, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
  • Predicted Molecular Mass
    22.6 kDa
    40-50 kDa, reducing conditions
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
1 μg/lane of Recombinant Human Fc gamma  RIIIA/CD16a was resolved with SDS‑PAGE under reducing (R) conditions and visualized by silver staining, showing bands at 40‑50 kDa.
Binding Activity
Recombinant Human Fc gamma  RIIIA/CD16a (Catalog # 4325-FC) binds human IgG with an estimated Kd 50 nM.
Background: Fc gamma RIIIA/CD16a

Fc gamma RIIIa is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity and clearance of immune complexes (1, 2). In humans, it is a 50-70 kDa type I transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages (1). Fc gamma RIIIb is highly related, sharing 97% amino acid (aa) identity within the extracellular domain (ECD), but is a GPI-linked receptor expressed on human neutrophils and eosinophils (1, 2). The ECD of Fc gamma RIIIa shares 63%, 61%, 65%, 59% and 58% aa identity with mouse Fc gamma RIV, rat Fc gamma RIIIa, feline CD16, bovine CD16 and porcine Fc gamma RIIIb paralogs, respectively. The Fc gamma RIIIa cDNA encodes 254 aa including a 16 aa signal sequence, 191 aa ECD with two C2-type Ig-like domains and five potential N-glycosylation sites, a 22 aa transmembrane (TM) sequence and a 25 aa cytoplasmic domain. In humans, a single nucleotide polymorphism creates high binding (176V) and low binding (176F) forms that, when homozygous, may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies (3, 4). Catalog # 4325-FC is expressed as the 176V isoform of Fc gamma RIIIa. Fc gamma RIIIa surface expression requires interaction of an accessory chain, either the common gamma -chain or CD3 zeta (5, 6). Glycosylation patterns, electrophoretic mobility and binding affinity appear to differ between NK cell and monocyte Fc gamma RIIIa (7). The ECD of both Fc gamma RIIIa and b can be proteolytically cleaved and retain binding activity in soluble form (8-11). In monocytes and macrophages, activation and phagocytosis can trigger Fc gamma RIIIa release (11). Soluble Fc gamma RIII can be detected in normal plasma and is increased in rheumatoid arthritis and in coronary artery diseases (9, 10).

  • References:
    1. Nimmerjahn, F. and J.V. Ravetch (2006) Immunity 24:19. 
    2. Ravetch, J.V. and B. Perussia (1989) J. Exp. Med. 170:481. 
    3. Wu, J. et al. (1997) J. Clin. Invest. 100:1059. 
    4. Dall’Ozzo, S. et al. (2004) Cancer Res. 64:4664. 
    5. Kim, M.-K. et al. (2003) Blood 101:4479. 
    6. Lanier, L.L. et al. (1989) Nature 342:803.
    7. Edberg, J.C. and R.P. Kimberley (1997) J. Immunol. 159:3849.
    8. Li, P. et al. (2007) J. Biol. Chem. 282:6210.
    9. Masuda, M. et al. (2003) J. Rheumatol. 30:1911.
    10. Masuda, M. et al. (2006) Atherosclerosis 188:377.
    11. Webster, N.L. et al. (2006) J. Leukoc. Biol. 79:294.
  • Long Name:
    Fc gamma Receptor III A
  • Entrez Gene IDs:
    2214 (Human)
  • Alternate Names:
    CD16a antigen; CD16a; CD16FCRIIIA; Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc gamma receptor III-A; FCG3; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; FCRIII; FcRIIIa; IGFR3FcgammaRIIIA; immunoglobulin G Fc receptor III; low affinity III, receptor for (CD16); low affinity immunoglobulin gamma Fc region receptor III-A; neutrophil-specific antigen NA
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. An Anti-EGFR IgA That Displays Improved Pharmacokinetics and Myeloid Effector Cell Engagement In Vivo.
    Authors: Lohse S, Meyer S, Meulenbroek L, Jansen J, Nederend M, Kretschmer A, Klausz K, Moginger U, Derer S, Rosner T, Kellner C, Schewe D, Sondermann P, Tiwari S, Kolarich D, Peipp M, Leusen J, Valerius T
    Cancer Res, 2016;76(2):403-17.
    Species: N/A
    Sample Type: Proteins
    Application: Surface Plasmon Resonance
  2. Mechanisms of protection against Clostridium difficile infection by the monoclonal antitoxin antibodies actoxumab and bezlotoxumab.
    Authors: Yang Z, Ramsey J, Hamza T, Zhang Y, Li S, Yfantis H, Lee D, Hernandez L, Seghezzi W, Furneisen J, Davis N, Therien A, Feng H
    Infect Immun, 2015;83(2):822-31.
    Species: Human
    Sample Type: Monoclonal Antibodies
    Application: Bioassay
  3. Engineering of a novel anti-CD40L domain antibody for treatment of autoimmune diseases.
    Authors: Xie J, Yamniuk A, Borowski V, Kuhn R, Susulic V, Rex-Rabe S, Yang X, Zhou X, Zhang Y, Gillooly K, Brosius R, Ravishankar R, Waggie K, Mink K, Price L, Rehfuss R, Tamura J, An Y, Cheng L, Abramczyk B, Ignatovich O, Drew P, Grant S, Bryson J, Suchard S, Salter-Cid L, Nadler S, Suri A
    J Immunol, 2014;192(9):4083-92.
    Species: Human
    Sample Type: Whole Tissue
    Application: Bioassay
  4. ADAM17-mediated shedding of FcgammaRIIIA on human NK cells: identification of the cleavage site and relationship with activation.
    Authors: Lajoie L, Congy-Jolivet N, Bolzec A, Gouilleux-Gruart V, Sicard E, Sung H, Peiretti F, Moreau T, Vie H, Clemenceau B, Thibault G
    J Immunol, 2014;192(2):741-51.
    Species: Human
    Sample Type: recombinant protein
    Application: Bioassay
  5. Bi-specific Aptamers Mediating Tumor Cell Lysis.
    Authors: Boltz A, Piater B, Toleikis L, Guenther R, Kolmar H, Hock B
    J. Biol. Chem., 2011;286(24):21896-905.
    Species: Human
    Sample Type: DNA Aptamer
    Application: EMSA
  6. Production of non-fucosylated antibodies by co-expression of heterologous GDP-6-deoxy-D-lyxo-4-hexulose reductase.
    Authors: von Horsten HH, Ogorek C, Blanchard V, Demmler C, Giese C, Winkler K, Kaup M, Berger M, Jordan I, Sandig V
    Glycobiology, 2010;20(12):1607-18.
    Species: Human
    Sample Type: Purified IgG
    Application: Bioassay
  7. Glycoengineering of human IgG1-Fc through combined yeast expression and in vitro chemoenzymatic glycosylation.
    Authors: Wei Y, Li C, Huang W, Li B, Strome S, Wang LX
    Biochemistry, 2008;47(39):10294-304.
    Species: Human
    Sample Type: Recombinant Human IgG
    Application: Surface Plasmon Resonance
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP MAB050 15  
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His Tag Horseradish Peroxidase-conjugated Antibody

WB, Simple Western MAB050H 2  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 1  
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His Tag Biotinylated Antibody

WB BAM050 1  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

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Average Rating: 5 (Based on 1 review)

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Images Ratings Applications Species Reviewed By Date Details
 ELISA Human Anonymous 10/26/2015


Sample TestedHuman recombinant test antibody

Other Experimental Details

Other Experimental DetailsSpecificity: Specific
Sensitivity: Sensitive
Buffer: TBS + Tween + Milk
Dilution: used at 0.25 ug/mL

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